Cross transferability of SSRs to five species of Araucariaceae: a useful tool for population genetic studies in Araucaria araucana
Cross-species amplification of microsatellites is a common procedure to obtain suitable markers to be used in population genetic studies. Primers designed for one (source) species are used to amplify homologous loci in related (target) species. It is expected that phylogenetically close species will...
| Autores principales: | , , , |
|---|---|
| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) (España)
2023
|
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/14978 https://revistas.inia.es/index.php/fs/article/view/1943 https://doi.org/10.5424/fs/2011202-11449 |
| _version_ | 1855485465676742656 |
|---|---|
| author | Moreno, Angela Carolina Marchelli, Paula Vendramin, Giovanni Giuseppe Gallo, Leonardo Ariel |
| author_browse | Gallo, Leonardo Ariel Marchelli, Paula Moreno, Angela Carolina Vendramin, Giovanni Giuseppe |
| author_facet | Moreno, Angela Carolina Marchelli, Paula Vendramin, Giovanni Giuseppe Gallo, Leonardo Ariel |
| author_sort | Moreno, Angela Carolina |
| collection | INTA Digital |
| description | Cross-species amplification of microsatellites is a common procedure to obtain suitable markers to be used in population genetic studies. Primers designed for one (source) species are used to amplify homologous loci in related (target) species. It is expected that phylogenetically close species will share a higher proportion of markers, and genetic distance could be a useful parameter to predict successful transferability between different taxonomic groups. We analyzed twenty-two primer pairs developed for Araucaria angustifolia (Bertol.) Kuntze in five target species of the Araucariaceae family. The results were summarized in vectors of presence and absence of bands and compared through the Jaccard similarity index. Using the sequences of eight published genes, genetic distances between pairs of species were estimated and related to transferability rate using Pearson correlations. Successful transfer rate ranged from 31.8 to 77.3%, being these among the highest reported for plants. The highest transfer rate was observed between the South American species. The transferability was confirmed sequencing seven fragments amplified in A. araucana (Molina) K. Koch, and using the best five to estimate genetic diversity parameters in a natural population of this Andean coniferous. |
| format | Artículo |
| id | INTA14978 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) (España) |
| publisherStr | Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) (España) |
| record_format | dspace |
| spelling | INTA149782023-08-22T14:20:24Z Cross transferability of SSRs to five species of Araucariaceae: a useful tool for population genetic studies in Araucaria araucana Moreno, Angela Carolina Marchelli, Paula Vendramin, Giovanni Giuseppe Gallo, Leonardo Ariel Transferencia de Genes Genética de Poblaciones Loci Gene Transfer Population Genetics Araucaria araucana Pehuen Cross-species amplification of microsatellites is a common procedure to obtain suitable markers to be used in population genetic studies. Primers designed for one (source) species are used to amplify homologous loci in related (target) species. It is expected that phylogenetically close species will share a higher proportion of markers, and genetic distance could be a useful parameter to predict successful transferability between different taxonomic groups. We analyzed twenty-two primer pairs developed for Araucaria angustifolia (Bertol.) Kuntze in five target species of the Araucariaceae family. The results were summarized in vectors of presence and absence of bands and compared through the Jaccard similarity index. Using the sequences of eight published genes, genetic distances between pairs of species were estimated and related to transferability rate using Pearson correlations. Successful transfer rate ranged from 31.8 to 77.3%, being these among the highest reported for plants. The highest transfer rate was observed between the South American species. The transferability was confirmed sequencing seven fragments amplified in A. araucana (Molina) K. Koch, and using the best five to estimate genetic diversity parameters in a natural population of this Andean coniferous. La transferencia de cebadores que amplifican loci microsatélites desde otras especies es una práctica habitual para obtener marcadores adecuados para estudios de genética poblacional. Los cebadores diseñados en una especie (fuente) son utilizados para amplificar loci homólogos en especies relacionadas (blanco). Se espera que especies cercanas filogenéticamente compartan un mayor número de marcadores y la distancia genética entre especies podría ser un parámetro útil para predecir el éxito de la transferencia entre diferentes grupos taxonómicos. Se analizó la transferencia de veintidós primers, desarrollados para Araucaria angustifolia (Bertol.) Kuntze, a cinco especies blanco de la familia Araucariaceae. Los resultados se resumieron en vectores de presencia y ausencia de bandas y fueron comparados a través del índice de similitud de Jaccard. Se estimaron las distancias genéticas de a pares entre las especies analizadas utilizando las secuencias de ocho genes, y se las relacionó con el índice de transferencia utilizando correlaciones de Pearson. El éxito de la transferencia varió entre 31,8 y 77,3%, encontrándose estos valores entre los más altos reportados en plantas. El mayor índice de transferencia se verificó entre las especies sudamericanas. La transferencia fue confirmada secuenciando siete fragmentos amplificados en Araucaria araucana (Molina) K. Koch, y utilizando los cinco mejores para estimar parámetros de diversidad genética en una población natural de esta conífera andina. EEA Bariloche Fil: Moreno, Angela Carolina. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estacion Experimental Agropecuaria Bariloche; Argentina Fil: Marchelli, Paula. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estacion Experimental Agropecuaria Bariloche; Argentina Fil: Marchelli, Paula. Consejo Nacional de Investigaciones Cientificas y Tecnicas; Argentina Fil: Vendramin, Giovanni Giuseppe. Istituto di Genetica Vegetale; Italia Fil: Gallo, Leonardo Ariel. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estacion Experimental Agropecuaria Bariloche; Argentina 2023-08-22T14:04:00Z 2023-08-22T14:04:00Z 2011 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/14978 https://revistas.inia.es/index.php/fs/article/view/1943 2171-9845 2171-5068 https://doi.org/10.5424/fs/2011202-11449 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) (España) Forest Systems 20 (2) : 303-314. (2011) |
| spellingShingle | Transferencia de Genes Genética de Poblaciones Loci Gene Transfer Population Genetics Araucaria araucana Pehuen Moreno, Angela Carolina Marchelli, Paula Vendramin, Giovanni Giuseppe Gallo, Leonardo Ariel Cross transferability of SSRs to five species of Araucariaceae: a useful tool for population genetic studies in Araucaria araucana |
| title | Cross transferability of SSRs to five species of Araucariaceae: a useful tool for population genetic studies in Araucaria araucana |
| title_full | Cross transferability of SSRs to five species of Araucariaceae: a useful tool for population genetic studies in Araucaria araucana |
| title_fullStr | Cross transferability of SSRs to five species of Araucariaceae: a useful tool for population genetic studies in Araucaria araucana |
| title_full_unstemmed | Cross transferability of SSRs to five species of Araucariaceae: a useful tool for population genetic studies in Araucaria araucana |
| title_short | Cross transferability of SSRs to five species of Araucariaceae: a useful tool for population genetic studies in Araucaria araucana |
| title_sort | cross transferability of ssrs to five species of araucariaceae a useful tool for population genetic studies in araucaria araucana |
| topic | Transferencia de Genes Genética de Poblaciones Loci Gene Transfer Population Genetics Araucaria araucana Pehuen |
| url | http://hdl.handle.net/20.500.12123/14978 https://revistas.inia.es/index.php/fs/article/view/1943 https://doi.org/10.5424/fs/2011202-11449 |
| work_keys_str_mv | AT morenoangelacarolina crosstransferabilityofssrstofivespeciesofaraucariaceaeausefultoolforpopulationgeneticstudiesinaraucariaaraucana AT marchellipaula crosstransferabilityofssrstofivespeciesofaraucariaceaeausefultoolforpopulationgeneticstudiesinaraucariaaraucana AT vendramingiovannigiuseppe crosstransferabilityofssrstofivespeciesofaraucariaceaeausefultoolforpopulationgeneticstudiesinaraucariaaraucana AT galloleonardoariel crosstransferabilityofssrstofivespeciesofaraucariaceaeausefultoolforpopulationgeneticstudiesinaraucariaaraucana |