ROS-Scavenging Enzymes as an Antioxidant Response to High Concentration of Anthracene in the Liverwort Marchantia polymorpha L.
Marchantia polymorpha L. responds to environmental changes using a myriad set of physiological responses, some unique to the lineage related to the lack of a vascular- and root-system. This study investigates the physiological response of M. polymorpha to high doses of anthracene analysing the antio...
| Autores principales: | , , , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
MDPI
2023
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/14970 https://www.mdpi.com/2223-7747/10/7/1478 https://doi.org/10.3390/plants10071478 |
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| author | Spinedi, Nahuel Storb, Romina Aranda, Elisabet Romani, Facundo Svriz, Maya Varela, Santiago Agustin Moreno, Javier E. Fracchia, Sebastian Cabrera, Juan Batista-García, Ramón Alberto Ponce de León, Inés Scervino, j. Martin |
| author_browse | Aranda, Elisabet Batista-García, Ramón Alberto Cabrera, Juan Fracchia, Sebastian Moreno, Javier E. Ponce de León, Inés Romani, Facundo Scervino, j. Martin Spinedi, Nahuel Storb, Romina Svriz, Maya Varela, Santiago Agustin |
| author_facet | Spinedi, Nahuel Storb, Romina Aranda, Elisabet Romani, Facundo Svriz, Maya Varela, Santiago Agustin Moreno, Javier E. Fracchia, Sebastian Cabrera, Juan Batista-García, Ramón Alberto Ponce de León, Inés Scervino, j. Martin |
| author_sort | Spinedi, Nahuel |
| collection | INTA Digital |
| description | Marchantia polymorpha L. responds to environmental changes using a myriad set of physiological responses, some unique to the lineage related to the lack of a vascular- and root-system. This study investigates the physiological response of M. polymorpha to high doses of anthracene analysing the antioxidant enzymes and their relationship with the photosynthetic processes, as well as their transcriptomic response. We found an anthracene dose-dependent response reducing plant biomass and associated to an alteration of the ultrastructure of a 23.6% of chloroplasts. Despite a reduction in total thallus-chlorophyll of 31.6% of Chl a and 38.4% of Chl b, this was not accompanied by a significant change in the net photosynthesis rate and maximum quantum efficiency (Fv/Fm). However, we found an increase in the activity of main ROS-detoxifying enzymes of 34.09% of peroxidase and 692% of ascorbate peroxidase, supported at transcriptional level with the upregulation of ROS-related detoxifying responses. Finally, we found that M. polymorpha tolerated anthracene-stress under the lowest concentration used and can suffer physiological alterations under higher concentrations tested related to the accumulation of anthracene within plant tissues. Our results show that M. polymorpha under PAH stress condition activated two complementary physiological responses including the activation of antioxidant mechanisms and the accumulation of the pollutant within plant tissues to mitigate the damage to the photosynthetic apparatus. |
| format | Artículo |
| id | INTA14970 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INTA149702023-08-22T11:45:03Z ROS-Scavenging Enzymes as an Antioxidant Response to High Concentration of Anthracene in the Liverwort Marchantia polymorpha L. Spinedi, Nahuel Storb, Romina Aranda, Elisabet Romani, Facundo Svriz, Maya Varela, Santiago Agustin Moreno, Javier E. Fracchia, Sebastian Cabrera, Juan Batista-García, Ramón Alberto Ponce de León, Inés Scervino, j. Martin Antioxidantes Enzimas Peroxidasas Hidrocarburos Aromáticos Policíclicos Secuencia de ARN Fotosíntesis Antioxidants Enzymes Peroxidases Polycyclic Aromatic Hydrocarbons RNA Sequence Photosynthesis Marchantia polymorpha L. responds to environmental changes using a myriad set of physiological responses, some unique to the lineage related to the lack of a vascular- and root-system. This study investigates the physiological response of M. polymorpha to high doses of anthracene analysing the antioxidant enzymes and their relationship with the photosynthetic processes, as well as their transcriptomic response. We found an anthracene dose-dependent response reducing plant biomass and associated to an alteration of the ultrastructure of a 23.6% of chloroplasts. Despite a reduction in total thallus-chlorophyll of 31.6% of Chl a and 38.4% of Chl b, this was not accompanied by a significant change in the net photosynthesis rate and maximum quantum efficiency (Fv/Fm). However, we found an increase in the activity of main ROS-detoxifying enzymes of 34.09% of peroxidase and 692% of ascorbate peroxidase, supported at transcriptional level with the upregulation of ROS-related detoxifying responses. Finally, we found that M. polymorpha tolerated anthracene-stress under the lowest concentration used and can suffer physiological alterations under higher concentrations tested related to the accumulation of anthracene within plant tissues. Our results show that M. polymorpha under PAH stress condition activated two complementary physiological responses including the activation of antioxidant mechanisms and the accumulation of the pollutant within plant tissues to mitigate the damage to the photosynthetic apparatus. EEA Bariloche Fil: Spinedi, Nahuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina Fil: Spinedi, Nahuel. Universidad Nacional del Comahue. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina Fil: Storb, Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina Fil: Storb, Romina. Universidad Nacional del Comahue. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina Fil: Aranda, Elisabet. University of Granada. Institute of Water Research; España Fil: Romani, Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología del Litoral; Argentina Fil: Romani, Facundo. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Instituto de Agrobiotecnología del Litoral; Argentina Fil: Romani, Facundo. University of Cambridge. Departament of Plant Sciences; Reino Unido Fil: Svriz, Maya. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Recursos Naturales, Agroecología y Desarrollo Rural; Argentina. Fil: Svriz, Maya. Universidad Nacional de Río Negro. Instituto de Investigaciones en Recursos Naturales, Agroecología y Desarrollo Rural; Argentina Fil: Varela, Santiago Agustín. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Barilohe. Grupo de Ecología Forestal; Argentina Fil: Moreno, Javier E.Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología del Litoral; Argentina Fil: Moreno, Javier E.Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Instituto de Agrobiotecnología del Litoral; Argentina Fil: Fracchia, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Regional de Investigaciones Científicas y Transferencia Tecnológica de La Rioja; Argentina Fil: Fracchia, Sebastian. Universidad Nacional de La Rioja. Centro Regional de Investigaciones Científicas y Transferencia Tecnológica de La Rioja; Argentina Fil: Fracchia, Sebastian. Servicio Geológico Minero Argentino. Centro Regional de Investigaciones Científicas y Transferencia Tecnológica de La Rioja; Argentina Fil: Fracchia, Sebastian. Universidad Nacional de Catamarca. Centro Regional de Investigaciones Científicas y Transferencia Tecnológica de La Rioja; Argentina Fil: Cabrera, Juan. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina Fil: Cabrera, Juan. Universidad Nacional del Comahue. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina Fil: Batista-García, Ramón Alberto. Universidad Autónoma del Estado de Morelos. Instituto de Investigación en Ciencias Básicas y Aplicadas. Centro de Investigación en Dinámica Celular; México Fil: Ponce de León: Instituto de Investigaciones Biológicas Clemente Estable. Departamento de Biología Molecular; Uruguay Fil: Scervino, J. Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina Fil: Scervino, J. Martin. Universidad Nacional del Comahue. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina 2023-08-22T11:28:47Z 2023-08-22T11:28:47Z 2021-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/14970 https://www.mdpi.com/2223-7747/10/7/1478 2223-7747 https://doi.org/10.3390/plants10071478 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Plants 10 (7) : 1478 (2021) |
| spellingShingle | Antioxidantes Enzimas Peroxidasas Hidrocarburos Aromáticos Policíclicos Secuencia de ARN Fotosíntesis Antioxidants Enzymes Peroxidases Polycyclic Aromatic Hydrocarbons RNA Sequence Photosynthesis Spinedi, Nahuel Storb, Romina Aranda, Elisabet Romani, Facundo Svriz, Maya Varela, Santiago Agustin Moreno, Javier E. Fracchia, Sebastian Cabrera, Juan Batista-García, Ramón Alberto Ponce de León, Inés Scervino, j. Martin ROS-Scavenging Enzymes as an Antioxidant Response to High Concentration of Anthracene in the Liverwort Marchantia polymorpha L. |
| title | ROS-Scavenging Enzymes as an Antioxidant Response to High Concentration of Anthracene in the Liverwort Marchantia polymorpha L. |
| title_full | ROS-Scavenging Enzymes as an Antioxidant Response to High Concentration of Anthracene in the Liverwort Marchantia polymorpha L. |
| title_fullStr | ROS-Scavenging Enzymes as an Antioxidant Response to High Concentration of Anthracene in the Liverwort Marchantia polymorpha L. |
| title_full_unstemmed | ROS-Scavenging Enzymes as an Antioxidant Response to High Concentration of Anthracene in the Liverwort Marchantia polymorpha L. |
| title_short | ROS-Scavenging Enzymes as an Antioxidant Response to High Concentration of Anthracene in the Liverwort Marchantia polymorpha L. |
| title_sort | ros scavenging enzymes as an antioxidant response to high concentration of anthracene in the liverwort marchantia polymorpha l |
| topic | Antioxidantes Enzimas Peroxidasas Hidrocarburos Aromáticos Policíclicos Secuencia de ARN Fotosíntesis Antioxidants Enzymes Peroxidases Polycyclic Aromatic Hydrocarbons RNA Sequence Photosynthesis |
| url | http://hdl.handle.net/20.500.12123/14970 https://www.mdpi.com/2223-7747/10/7/1478 https://doi.org/10.3390/plants10071478 |
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