Deciphering the transcriptomic regulation of heat stress responses in Nothofagus pumilio
Global warming is predicted to exert negative impacts on plant growth due to the damaging effect of high temperatures on plant physiology. Revealing the genetic architecture underly ing the heat stress response is therefore crucial for the development of conservation strate gies, and for breeding h...
| Main Authors: | , , , , , |
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
| Published: |
Public Library of Science (PLOS)
2023
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/14909 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0246615 https://doi.org/10.1371/journal.pone.0246615 |
| Summary: | Global warming is predicted to exert negative impacts on plant growth due to the damaging
effect of high temperatures on plant physiology. Revealing the genetic architecture underly ing the heat stress response is therefore crucial for the development of conservation strate gies, and for breeding heat-resistant plant genotypes. Here we investigated the
transcriptional changes induced by heat in Nothofagus pumilio, an emblematic tree species
of the sub-Antarctic forests of South America. Through the performance of RNA-seq of
leaves of plants exposed to 20˚C (control) or 34˚C (heat shock), we generated the first tran scriptomic resource for the species. We also studied the changes in protein-coding tran scripts expression in response to heat. We found 5,214 contigs differentially expressed
between temperatures. The heat treatment resulted in a down-regulation of genes related to
photosynthesis and carbon metabolism, whereas secondary metabolism, protein re-folding
and response to stress were up-regulated. Moreover, several transcription factor families
like WRKY or ERF were promoted by heat, alongside spliceosome machinery and hormone
signaling pathways. Through a comparative analysis of gene regulation in response to heat
in Arabidopsis thaliana, Populus tomentosa and N. pumilio we provide evidence of the exis tence of shared molecular features of heat stress responses across angiosperms, and iden tify genes of potential biotechnological application. |
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