Functional characterization of DcMYB11, an R2R3 MYB associated with the purple pigmentation of carrot petiole
The accumulation of anthocyanin pigments can exhibit different patterns across plant tissues and crop varieties. This variability allowed the investigation of the molecular mechanisms behind the biosynthesis of these pigments in several plant species. Among crops, carrots have a well-defined anthocy...
| Autores principales: | , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Springer
2023
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/14846 https://link.springer.com/article/10.1007/s00425-023-04200-5 https://doi.org/10.1007/s00425-023-04200-5 |
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| author | D’Amelia, Vincenzo Curaba, Julien Abid, Muhammad Ali Esposito, Salvatore Cavagnaro, Pablo Carputo, Domenico Iorizzo, Massimo |
| author_browse | Abid, Muhammad Ali Carputo, Domenico Cavagnaro, Pablo Curaba, Julien D’Amelia, Vincenzo Esposito, Salvatore Iorizzo, Massimo |
| author_facet | D’Amelia, Vincenzo Curaba, Julien Abid, Muhammad Ali Esposito, Salvatore Cavagnaro, Pablo Carputo, Domenico Iorizzo, Massimo |
| author_sort | D’Amelia, Vincenzo |
| collection | INTA Digital |
| description | The accumulation of anthocyanin pigments can exhibit different patterns across plant tissues and crop varieties. This variability allowed the investigation of the molecular mechanisms behind the biosynthesis of these pigments in several plant species. Among crops, carrots have a well-defined anthocyanin pigmentation pattern depending on the genic background. In this work, we report on the discovery of DNA structural differences affecting the activity of an R2R3 MYB (encoded by DcMYB11) involved in anthocyanin regulation in carrot petiole. To this end, we first verified the function of DcMYB11 using heterologous systems and identified three different alleles which may explain differences in petiole pigmentation. Characterization of the DcMYB11 alleles at the 5' upstream sequence unveiled a sequence that functions as a putative enhancer. In conclusion, this study provides novel insight into the molecular mechanisms controlling anthocyanin accumulation in carrot. By these outcomes, we expanded our knowledge on the cis-regulatory sequences in plants. |
| format | info:ar-repo/semantics/artículo |
| id | INTA14846 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA148462023-08-01T11:30:09Z Functional characterization of DcMYB11, an R2R3 MYB associated with the purple pigmentation of carrot petiole D’Amelia, Vincenzo Curaba, Julien Abid, Muhammad Ali Esposito, Salvatore Cavagnaro, Pablo Carputo, Domenico Iorizzo, Massimo Zanahoria Pigmentación Variedades Daucus carota Antocianinas Carrots Pigmentation Varieties Anthocyanins Zanahoria Morada Purple Carrots The accumulation of anthocyanin pigments can exhibit different patterns across plant tissues and crop varieties. This variability allowed the investigation of the molecular mechanisms behind the biosynthesis of these pigments in several plant species. Among crops, carrots have a well-defined anthocyanin pigmentation pattern depending on the genic background. In this work, we report on the discovery of DNA structural differences affecting the activity of an R2R3 MYB (encoded by DcMYB11) involved in anthocyanin regulation in carrot petiole. To this end, we first verified the function of DcMYB11 using heterologous systems and identified three different alleles which may explain differences in petiole pigmentation. Characterization of the DcMYB11 alleles at the 5' upstream sequence unveiled a sequence that functions as a putative enhancer. In conclusion, this study provides novel insight into the molecular mechanisms controlling anthocyanin accumulation in carrot. By these outcomes, we expanded our knowledge on the cis-regulatory sequences in plants. EEA La Consulta Fil: D’Amelia, Vincenzo. National Research Council. Institute of Bioscience and BioResources; Italia Fil: D’Amelia, Vincenzo. University of Naples Federico II. Department of Agricultural Sciences; Italia Fil: Curaba, Julien. North Carolina State University. Horticulture Science Department. Plants for Human Health Institute; Estados Unidos Fil: Abid, Muhammad Ali. North Carolina State University. Horticulture Science Department. Plants for Human Health Institute; Estados Unidos Fil: Esposito, Salvatore. CREA Research Centre for Cereal and Industrial Crops; Italia Fil: Cavagnaro, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Cavagnaro, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Fil: Carputo, Domenico. University of Naples Federico II. Department of Agricultural Sciences; Italia Fil: Iorizzo, Massimo. North Carolina State University. Horticulture Science Department. Plants for Human Health Institute; Estados Unidos 2023-08-01T11:26:52Z 2023-08-01T11:26:52Z 2023-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/14846 https://link.springer.com/article/10.1007/s00425-023-04200-5 0032-0935 1432-2048 https://doi.org/10.1007/s00425-023-04200-5 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Planta 258 : Article number: 50 (July 2023) |
| spellingShingle | Zanahoria Pigmentación Variedades Daucus carota Antocianinas Carrots Pigmentation Varieties Anthocyanins Zanahoria Morada Purple Carrots D’Amelia, Vincenzo Curaba, Julien Abid, Muhammad Ali Esposito, Salvatore Cavagnaro, Pablo Carputo, Domenico Iorizzo, Massimo Functional characterization of DcMYB11, an R2R3 MYB associated with the purple pigmentation of carrot petiole |
| title | Functional characterization of DcMYB11, an R2R3 MYB associated with the purple pigmentation of carrot petiole |
| title_full | Functional characterization of DcMYB11, an R2R3 MYB associated with the purple pigmentation of carrot petiole |
| title_fullStr | Functional characterization of DcMYB11, an R2R3 MYB associated with the purple pigmentation of carrot petiole |
| title_full_unstemmed | Functional characterization of DcMYB11, an R2R3 MYB associated with the purple pigmentation of carrot petiole |
| title_short | Functional characterization of DcMYB11, an R2R3 MYB associated with the purple pigmentation of carrot petiole |
| title_sort | functional characterization of dcmyb11 an r2r3 myb associated with the purple pigmentation of carrot petiole |
| topic | Zanahoria Pigmentación Variedades Daucus carota Antocianinas Carrots Pigmentation Varieties Anthocyanins Zanahoria Morada Purple Carrots |
| url | http://hdl.handle.net/20.500.12123/14846 https://link.springer.com/article/10.1007/s00425-023-04200-5 https://doi.org/10.1007/s00425-023-04200-5 |
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