A simple method to select high superovulatory responder goats
In most female mammals, a common drawback to multiple ovulation embryo transfer programs is the variability in the superovulatory response to the multidose pFSH treatment. The aim of the present study was to identify embryo donor goats based on their response to superovulation before the performance...
| Autores principales: | , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2023
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/14841 https://www.sciencedirect.com/science/article/pii/S0093691X22004460 https://doi.org/10.1016/j.theriogenology.2022.10.032 |
| _version_ | 1855037192850636800 |
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| author | Bruno Galarraga, María Macarena Fernandez, Jimena Lacau Megido, Isabel María Gonzalez-Bulnes, Antonio Gibbons, Alejandro Eduardo Cueto, Marcela Isabel |
| author_browse | Bruno Galarraga, María Macarena Cueto, Marcela Isabel Fernandez, Jimena Gibbons, Alejandro Eduardo Gonzalez-Bulnes, Antonio Lacau Megido, Isabel María |
| author_facet | Bruno Galarraga, María Macarena Fernandez, Jimena Lacau Megido, Isabel María Gonzalez-Bulnes, Antonio Gibbons, Alejandro Eduardo Cueto, Marcela Isabel |
| author_sort | Bruno Galarraga, María Macarena |
| collection | INTA Digital |
| description | In most female mammals, a common drawback to multiple ovulation embryo transfer programs is the variability in the superovulatory response to the multidose pFSH treatment. The aim of the present study was to identify embryo donor goats based on their response to superovulation before the performance of a high-cost hormonal treatment, as we have previously done in sheep. To this end, we evaluated the number of ovulations obtained in response to the administration of a one-shot eCG treatment and related it with the subsequent ovarian response to a multiple-dose pFSH treatment in 33 goats of the Criolla-Neuquina breed. Goats received a one-shot eCG treatment of 800 IU at the end of a 17-day progestational treatment; 9 days later, started a second 17-day progestational treatment and then received a multiple-dose pFSH treatment on days 15–17 (116 mg pFSH, in six decreasing doses). The number of corpora lutea (CL) per goat was recorded laparoscopically after both hormonal treatments. On day 8 after the second pessary removal, embryos were surgically recovered and classified by quality. Results showed a significant positive correlation between the number of CL obtained in response to the eCG and pFSH treatments (r = 0.41; y = 0.8352x + 6.9906; P < 0.05), although of limited value to select high ovulatory responding goats, due to its low correlation value. Then, goats were grouped into high and low ovulatory responders to the eCG (High ≥9; Low <9 CL) and pFSH treatments (High ≥13; Low <13 CL). After the eCG and pFSH treatments, 60% of the goats maintained their classification as high or low superovulatory responders (expressed as recurrence rate). Significant differences were found in the number of CL (18.2 ± 1.3 vs 9.9 ± 1.3), number of embryos + oocytes (13.5 ± 1.7 vs 7.6 ± 1.7), number of embryos (10.8 ± 1.4 vs 5.1 ± 1.4) and number of Grade 1 and Grade 2 embryos (8.8 ± 1.4 vs 4.3 ± 1.4) between high and low superovulatory responder goats (P < 0.05), while no differences were observed in the number of oocytes and in the recovery of embryos + oocytes, embryos, Grade 1 and Grade 2 embryos and fertilization rates (P > 0.05). In conclusion, the recurrence rate in ovarian response between the one-shot eCG treatment and the multiple-dose pFSH treatment would confirm the existence of an “individual or intrinsic factor” of the donor goat that would respond as a high or low ovulatory responder to superovulatory treatments. |
| format | info:ar-repo/semantics/artículo |
| id | INTA14841 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA148412023-07-31T11:15:37Z A simple method to select high superovulatory responder goats Bruno Galarraga, María Macarena Fernandez, Jimena Lacau Megido, Isabel María Gonzalez-Bulnes, Antonio Gibbons, Alejandro Eduardo Cueto, Marcela Isabel Caprinos Reproducción Animal Superovulación Métodos Goats Animal Reproduction Superovulation Methods In most female mammals, a common drawback to multiple ovulation embryo transfer programs is the variability in the superovulatory response to the multidose pFSH treatment. The aim of the present study was to identify embryo donor goats based on their response to superovulation before the performance of a high-cost hormonal treatment, as we have previously done in sheep. To this end, we evaluated the number of ovulations obtained in response to the administration of a one-shot eCG treatment and related it with the subsequent ovarian response to a multiple-dose pFSH treatment in 33 goats of the Criolla-Neuquina breed. Goats received a one-shot eCG treatment of 800 IU at the end of a 17-day progestational treatment; 9 days later, started a second 17-day progestational treatment and then received a multiple-dose pFSH treatment on days 15–17 (116 mg pFSH, in six decreasing doses). The number of corpora lutea (CL) per goat was recorded laparoscopically after both hormonal treatments. On day 8 after the second pessary removal, embryos were surgically recovered and classified by quality. Results showed a significant positive correlation between the number of CL obtained in response to the eCG and pFSH treatments (r = 0.41; y = 0.8352x + 6.9906; P < 0.05), although of limited value to select high ovulatory responding goats, due to its low correlation value. Then, goats were grouped into high and low ovulatory responders to the eCG (High ≥9; Low <9 CL) and pFSH treatments (High ≥13; Low <13 CL). After the eCG and pFSH treatments, 60% of the goats maintained their classification as high or low superovulatory responders (expressed as recurrence rate). Significant differences were found in the number of CL (18.2 ± 1.3 vs 9.9 ± 1.3), number of embryos + oocytes (13.5 ± 1.7 vs 7.6 ± 1.7), number of embryos (10.8 ± 1.4 vs 5.1 ± 1.4) and number of Grade 1 and Grade 2 embryos (8.8 ± 1.4 vs 4.3 ± 1.4) between high and low superovulatory responder goats (P < 0.05), while no differences were observed in the number of oocytes and in the recovery of embryos + oocytes, embryos, Grade 1 and Grade 2 embryos and fertilization rates (P > 0.05). In conclusion, the recurrence rate in ovarian response between the one-shot eCG treatment and the multiple-dose pFSH treatment would confirm the existence of an “individual or intrinsic factor” of the donor goat that would respond as a high or low ovulatory responder to superovulatory treatments. EEA Bariloche Fil: Bruno Galarraga, María Macarena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Area Producción Animal; Argentina Fil: Bruno Galarraga, María Macarena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina Fil: Fernandez, Jimena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Area Producción Animal; Argentina Fil: Fernandez, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina Fil: Lacau Megido, Isabel María. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Biología y Medicina Experimental. Laboratorio de Regulación Hipofisaria; Argentina Fil: Gonzalez-Bulnes, Antonio. Universidad Cardenal-Herrera. Facultad de Veterinaria. Departamento de Producción y Sanidad Animal; España Fil: Gibbons, Alejandro Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Area Producción Animal; Argentina Fil: Gibbons, Alejandro Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina Fil: Cueto, Marcela Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Area Producción Animal; Argentina Fil: Cueto, Marcela Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina 2023-07-31T11:11:49Z 2023-07-31T11:11:49Z 2023-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/14841 https://www.sciencedirect.com/science/article/pii/S0093691X22004460 0093-691X 1879-3231 https://doi.org/10.1016/j.theriogenology.2022.10.032 eng info:eu-repograntAgreement/INTA/2019-PD-E5-I107-001/2019-PD-E5-I107-001, Biotecnologías de la reproducción: mejora de la eficiencia reproductiva en especies de interés zootécnico info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Theriogenology 195 : 187-191. (January 2023) |
| spellingShingle | Caprinos Reproducción Animal Superovulación Métodos Goats Animal Reproduction Superovulation Methods Bruno Galarraga, María Macarena Fernandez, Jimena Lacau Megido, Isabel María Gonzalez-Bulnes, Antonio Gibbons, Alejandro Eduardo Cueto, Marcela Isabel A simple method to select high superovulatory responder goats |
| title | A simple method to select high superovulatory responder goats |
| title_full | A simple method to select high superovulatory responder goats |
| title_fullStr | A simple method to select high superovulatory responder goats |
| title_full_unstemmed | A simple method to select high superovulatory responder goats |
| title_short | A simple method to select high superovulatory responder goats |
| title_sort | simple method to select high superovulatory responder goats |
| topic | Caprinos Reproducción Animal Superovulación Métodos Goats Animal Reproduction Superovulation Methods |
| url | http://hdl.handle.net/20.500.12123/14841 https://www.sciencedirect.com/science/article/pii/S0093691X22004460 https://doi.org/10.1016/j.theriogenology.2022.10.032 |
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