Closing the Gaps to Understand the Tick Transmission of Anaplasma marginale among Giant Anteaters (Myrmecophaga tridactyla) in Argentina
Anaplasma marginale, a well-known cattle pathogen of tropical and subtropical world regions, has been previously molecularly characterized in a giant anteater (Myrmecophaga tridactyla) from Corrientes, Argentina. Ticks or other hematophagous arthropod involved in the wild transmission cycle remained...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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MDPI
2023
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| Online Access: | http://hdl.handle.net/20.500.12123/14827 https://www.mdpi.com/2076-0817/9/12/1033 https://doi.org/10.3390/pathogens9121033 |
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| author | Guillemi, Eliana Carolina Imbert, Mélody De La Fourniere, Sofia Orozco, Maria Marcela Peña Martínez, Jorge Rosas, Ana Carolina Montenegro, Valeria Noely Farber, Marisa Diana |
| author_browse | De La Fourniere, Sofia Farber, Marisa Diana Guillemi, Eliana Carolina Imbert, Mélody Montenegro, Valeria Noely Orozco, Maria Marcela Peña Martínez, Jorge Rosas, Ana Carolina |
| author_facet | Guillemi, Eliana Carolina Imbert, Mélody De La Fourniere, Sofia Orozco, Maria Marcela Peña Martínez, Jorge Rosas, Ana Carolina Montenegro, Valeria Noely Farber, Marisa Diana |
| author_sort | Guillemi, Eliana Carolina |
| collection | INTA Digital |
| description | Anaplasma marginale, a well-known cattle pathogen of tropical and subtropical world regions, has been previously molecularly characterized in a giant anteater (Myrmecophaga tridactyla) from Corrientes, Argentina. Ticks or other hematophagous arthropod involved in the wild transmission cycle remained unknown. The aim of the present study was to analyze the simultaneous occurrence of A. marginale in blood samples and ticks from giant anteaters from Corrientes in order to investigate if ticks could be relevant in the transmission among these mammals. Blood samples from 50 giant anteaters collected in different years and 26 ticks Amblyomma dubitatum and A. sculptum were studied through the molecular amplification of two unequivocal species-specific genes from A. marginale: msp5 and msp1β. Twenty five giant anteaters and tick organs (salivary glands, gut and oviduct) from 11 ticks tested positive to the A. marginale DNA amplification. The further molecular characterization through MSP1a tandem repeats analysis revealed the presence of genotypes circulating among giant anteaters that had been previously identified in cattle blood samples from the same geographical region. These results confirm the presence of A. marginale in giant anteaters in Corrientes and suggests that A. dubitatum and A. sculptum ticks could be involved in the transmission among giant anteaters. Future studies will determine the role of these tick species in the wild transmission cycle in the study area and the eventual connection with the domestic cycle. |
| format | info:ar-repo/semantics/artículo |
| id | INTA14827 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INTA148272023-07-27T12:33:57Z Closing the Gaps to Understand the Tick Transmission of Anaplasma marginale among Giant Anteaters (Myrmecophaga tridactyla) in Argentina Guillemi, Eliana Carolina Imbert, Mélody De La Fourniere, Sofia Orozco, Maria Marcela Peña Martínez, Jorge Rosas, Ana Carolina Montenegro, Valeria Noely Farber, Marisa Diana Anaplasma marginale Anteaters Amblyomma Pests of Animals Oso Hormiguero Plagas de Animales Argentina Garrapatas Myrmecophaga tridactyla Amblyomma dubitatum Amblyomma sculptum Ticks Anaplasma marginale, a well-known cattle pathogen of tropical and subtropical world regions, has been previously molecularly characterized in a giant anteater (Myrmecophaga tridactyla) from Corrientes, Argentina. Ticks or other hematophagous arthropod involved in the wild transmission cycle remained unknown. The aim of the present study was to analyze the simultaneous occurrence of A. marginale in blood samples and ticks from giant anteaters from Corrientes in order to investigate if ticks could be relevant in the transmission among these mammals. Blood samples from 50 giant anteaters collected in different years and 26 ticks Amblyomma dubitatum and A. sculptum were studied through the molecular amplification of two unequivocal species-specific genes from A. marginale: msp5 and msp1β. Twenty five giant anteaters and tick organs (salivary glands, gut and oviduct) from 11 ticks tested positive to the A. marginale DNA amplification. The further molecular characterization through MSP1a tandem repeats analysis revealed the presence of genotypes circulating among giant anteaters that had been previously identified in cattle blood samples from the same geographical region. These results confirm the presence of A. marginale in giant anteaters in Corrientes and suggests that A. dubitatum and A. sculptum ticks could be involved in the transmission among giant anteaters. Future studies will determine the role of these tick species in the wild transmission cycle in the study area and the eventual connection with the domestic cycle. Instituto de Biotecnología Fil: Guillemi, Eliana Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Guillemi, Eliana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Imbert, Mélody. Université des Sciences Paul Sabatier; Francia Fil: De La Fourniere, Sofía Ana María. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: De La Fourniere, Sofía Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Orozco, Maria Marcela. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina Fil: Orozco, Maria Marcela Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina Fil: Peña Martínez, Jorge. The Conservation Land Trust; Argentina Fil: Rosas, Ana Carolina. The Conservation Land Trust; Argentina Fil: Montenegro, Valeria Noely. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Montenegro, Valeria Noely. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Farber, Marisa Diana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Farber, Marisa Diana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2023-07-27T12:31:28Z 2023-07-27T12:31:28Z 2020-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/14827 https://www.mdpi.com/2076-0817/9/12/1033 2076-0817 https://doi.org/10.3390/pathogens9121033 eng info:eu-repograntAgreement/INTA/PNBIO-1131043/AR./Bioinformática y Estadística Genómica. info:eu-repograntAgreement/INTA/PNBIO-1131032/AR./Desarrollo de herramientas biotecnológicas para la prevención y el control de enfermedades pecuarias: vacunas, diagnóstico y eIdemiología molecular. info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Argentina .......... (nation) (World, South America) 7006477 MDPI Pathogens 9 (12) : 1033. (December 2020) |
| spellingShingle | Anaplasma marginale Anteaters Amblyomma Pests of Animals Oso Hormiguero Plagas de Animales Argentina Garrapatas Myrmecophaga tridactyla Amblyomma dubitatum Amblyomma sculptum Ticks Guillemi, Eliana Carolina Imbert, Mélody De La Fourniere, Sofia Orozco, Maria Marcela Peña Martínez, Jorge Rosas, Ana Carolina Montenegro, Valeria Noely Farber, Marisa Diana Closing the Gaps to Understand the Tick Transmission of Anaplasma marginale among Giant Anteaters (Myrmecophaga tridactyla) in Argentina |
| title | Closing the Gaps to Understand the Tick Transmission of Anaplasma marginale among Giant Anteaters (Myrmecophaga tridactyla) in Argentina |
| title_full | Closing the Gaps to Understand the Tick Transmission of Anaplasma marginale among Giant Anteaters (Myrmecophaga tridactyla) in Argentina |
| title_fullStr | Closing the Gaps to Understand the Tick Transmission of Anaplasma marginale among Giant Anteaters (Myrmecophaga tridactyla) in Argentina |
| title_full_unstemmed | Closing the Gaps to Understand the Tick Transmission of Anaplasma marginale among Giant Anteaters (Myrmecophaga tridactyla) in Argentina |
| title_short | Closing the Gaps to Understand the Tick Transmission of Anaplasma marginale among Giant Anteaters (Myrmecophaga tridactyla) in Argentina |
| title_sort | closing the gaps to understand the tick transmission of anaplasma marginale among giant anteaters myrmecophaga tridactyla in argentina |
| topic | Anaplasma marginale Anteaters Amblyomma Pests of Animals Oso Hormiguero Plagas de Animales Argentina Garrapatas Myrmecophaga tridactyla Amblyomma dubitatum Amblyomma sculptum Ticks |
| url | http://hdl.handle.net/20.500.12123/14827 https://www.mdpi.com/2076-0817/9/12/1033 https://doi.org/10.3390/pathogens9121033 |
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