Microevolution rather than large genome divergence determines the effectiveness of legume–rhizobia symbiotic interaction under field conditions
Despite the vast screening for natural nitrogenfixing isolates by public and private consortia, no significant progresses in the production of improved nitrogenfixing inoculants for alfalfa production have been made in the last years. Here, we present a comprehensive characterization of the nitrogen...
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| Format: | Artículo |
| Language: | Inglés |
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2017
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| Online Access: | http://hdl.handle.net/20.500.12123/1474 https://link.springer.com/article/10.1007/s00239-017-9808-6 https://doi.org/10.1007/s00239-017-9808-6 |
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| author | Jozefkowicz, Cintia Brambilla, Silvina Maricel Frare, Romina Alejandra Stritzler, Margarita Puente, Mariana Laura Piccinetti, Carlos Fabian Soto, Gabriela Cinthia Ayub, Nicolás Daniel |
| author_browse | Ayub, Nicolás Daniel Brambilla, Silvina Maricel Frare, Romina Alejandra Jozefkowicz, Cintia Piccinetti, Carlos Fabian Puente, Mariana Laura Soto, Gabriela Cinthia Stritzler, Margarita |
| author_facet | Jozefkowicz, Cintia Brambilla, Silvina Maricel Frare, Romina Alejandra Stritzler, Margarita Puente, Mariana Laura Piccinetti, Carlos Fabian Soto, Gabriela Cinthia Ayub, Nicolás Daniel |
| author_sort | Jozefkowicz, Cintia |
| collection | INTA Digital |
| description | Despite the vast screening for natural nitrogenfixing isolates by public and private consortia, no significant progresses in the production of improved nitrogenfixing inoculants for alfalfa production have been made in the last years. Here, we present a comprehensive characterization of the nitrogen-fixing strain Ensifer meliloti
B399 (originally named Rhizobium meliloti 102F34), probably the inoculant most widely used in alfalfa production since the 1960s. Complete nucleotide sequence and
genome analysis of strain B399 showed that the three replicons present in this commercial strain and the model bacterium Ensifer meliloti 1021 are extremely similar to each other in terms of nucleotide identity and synteny conservation. In contrast to that observed in B399-treated plants, inoculation of plants with strain 1021 did not
improve nitrogen content in different alfalfa cultivars under field conditions, suggesting that a small genomic divergence can drastically impact on the symbiotic phenotype. Therefore, in addition to the traditional screening of natural
nitrogen-fixing isolates, the genome engineering of model strains could be an attractive strategy to improve nitrogen fixation in legume crops. |
| format | Artículo |
| id | INTA1474 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA14742018-03-07T13:51:37Z Microevolution rather than large genome divergence determines the effectiveness of legume–rhizobia symbiotic interaction under field conditions Jozefkowicz, Cintia Brambilla, Silvina Maricel Frare, Romina Alejandra Stritzler, Margarita Puente, Mariana Laura Piccinetti, Carlos Fabian Soto, Gabriela Cinthia Ayub, Nicolás Daniel Genética Leguminosas Rhizobium Genomas Divergencia Genética Genetics Legumes Genomes Genetic Divergence Despite the vast screening for natural nitrogenfixing isolates by public and private consortia, no significant progresses in the production of improved nitrogenfixing inoculants for alfalfa production have been made in the last years. Here, we present a comprehensive characterization of the nitrogen-fixing strain Ensifer meliloti B399 (originally named Rhizobium meliloti 102F34), probably the inoculant most widely used in alfalfa production since the 1960s. Complete nucleotide sequence and genome analysis of strain B399 showed that the three replicons present in this commercial strain and the model bacterium Ensifer meliloti 1021 are extremely similar to each other in terms of nucleotide identity and synteny conservation. In contrast to that observed in B399-treated plants, inoculation of plants with strain 1021 did not improve nitrogen content in different alfalfa cultivars under field conditions, suggesting that a small genomic divergence can drastically impact on the symbiotic phenotype. Therefore, in addition to the traditional screening of natural nitrogen-fixing isolates, the genome engineering of model strains could be an attractive strategy to improve nitrogen fixation in legume crops. Inst. de Microbiología y Zoología Agrícola IMyZA Fil: Jozefkowicz, Cintia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Brambilla, Silvina Maricel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina Fil: Frare, Romina Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Stritzler, Margarita. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Puente, Mariana Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Piccinetti, Carlos Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2017-10-12T13:28:05Z 2017-10-12T13:28:05Z 2017-08 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/1474 https://link.springer.com/article/10.1007/s00239-017-9808-6 0022-2844 (Print) 1432-1432 (Online) https://doi.org/10.1007/s00239-017-9808-6 eng info:eu-repo/semantics/restrictedAccess application/pdf Journal of molecular evolution 85 (3-4) : 79–83 (October 2017) |
| spellingShingle | Genética Leguminosas Rhizobium Genomas Divergencia Genética Genetics Legumes Genomes Genetic Divergence Jozefkowicz, Cintia Brambilla, Silvina Maricel Frare, Romina Alejandra Stritzler, Margarita Puente, Mariana Laura Piccinetti, Carlos Fabian Soto, Gabriela Cinthia Ayub, Nicolás Daniel Microevolution rather than large genome divergence determines the effectiveness of legume–rhizobia symbiotic interaction under field conditions |
| title | Microevolution rather than large genome divergence determines the effectiveness of legume–rhizobia symbiotic interaction under field conditions |
| title_full | Microevolution rather than large genome divergence determines the effectiveness of legume–rhizobia symbiotic interaction under field conditions |
| title_fullStr | Microevolution rather than large genome divergence determines the effectiveness of legume–rhizobia symbiotic interaction under field conditions |
| title_full_unstemmed | Microevolution rather than large genome divergence determines the effectiveness of legume–rhizobia symbiotic interaction under field conditions |
| title_short | Microevolution rather than large genome divergence determines the effectiveness of legume–rhizobia symbiotic interaction under field conditions |
| title_sort | microevolution rather than large genome divergence determines the effectiveness of legume rhizobia symbiotic interaction under field conditions |
| topic | Genética Leguminosas Rhizobium Genomas Divergencia Genética Genetics Legumes Genomes Genetic Divergence |
| url | http://hdl.handle.net/20.500.12123/1474 https://link.springer.com/article/10.1007/s00239-017-9808-6 https://doi.org/10.1007/s00239-017-9808-6 |
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