Grape Stalk-Based Extracts Controlling Fruit Pathogenic Fungi as a Waste Biomass Valorization Alternative in Winemaking
Grape stalks—the lignocellulosic skeleton of the grape raceme- are accumulated in massive piles immediately after harvest and become a concentrated pollutant. Fungi cause several rots in grapevine and other fruit cultivars and albeit biocontrol agents are available, superior technologies must be dev...
| Autores principales: | , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Springer
2023
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/14729 https://link.springer.com/article/10.1007/s12649-021-01533-8 https://doi.org/10.1007/s12649-021-01533-8 |
| _version_ | 1855485419124162560 |
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| author | Palazzolo, Martín A. Aballay, Maximiliano Martín Martínez, Antares A. Kurina-Sanz, Marcela |
| author_browse | Aballay, Maximiliano Martín Kurina-Sanz, Marcela Martínez, Antares A. Palazzolo, Martín A. |
| author_facet | Palazzolo, Martín A. Aballay, Maximiliano Martín Martínez, Antares A. Kurina-Sanz, Marcela |
| author_sort | Palazzolo, Martín A. |
| collection | INTA Digital |
| description | Grape stalks—the lignocellulosic skeleton of the grape raceme- are accumulated in massive piles immediately after harvest and become a concentrated pollutant. Fungi cause several rots in grapevine and other fruit cultivars and albeit biocontrol agents are available, superior technologies must be developed to rival synthetic counterparts. In this work, we describe the preparation of grape stalk lipophilic extracts from Malbec, Cabernet sauvignon, Chardonnay and Pedro Giménez as biocontrol tools against Aspergillus candidus, Penicillium chrysogenum, and Rhizopus sp., which are pathogenic for grapes and other fruits. Grape stalk extracts were obtained with hexane, diethyl ether, ethyl acetate, and ethanol. Yields were in the range of 1 to 23% w/w according to solvent polarity. Extracts were separated into lipophilic sub-extracts—yields varied from 70 up to 12% w/w due to solvent polarity. Sub-extracts were assayed for antifungal activity in vitro and the one obtained from Pedro Giménez with ethanol exhibited the broadest biocontrol spectrum at 2.5 × 104 mg/L. This sub-extract was separated into components by column chromatography to inquire further on its biocontrol activity. A bioactivity-guided test against Rhizopus sp. revealed that this effect relies on gamma-sitosterol and a biocontrol cut-off value of 5.1 × 103 mg/L was estimated. |
| format | Artículo |
| id | INTA14729 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA147292023-07-13T17:24:26Z Grape Stalk-Based Extracts Controlling Fruit Pathogenic Fungi as a Waste Biomass Valorization Alternative in Winemaking Palazzolo, Martín A. Aballay, Maximiliano Martín Martínez, Antares A. Kurina-Sanz, Marcela Desechos Agrícolas Propiedades Antimicosicas Control Biológico Uva Bioplaguicidas Control de Enfermedades de Plantas Agricultural Wastes Antifungal Properties Biological Control Grapes Biopesticides Plant Disease Control Grape Stalks Plant Sterol Escobajo Esterol Vegetal Raquis de Uva Grape stalks—the lignocellulosic skeleton of the grape raceme- are accumulated in massive piles immediately after harvest and become a concentrated pollutant. Fungi cause several rots in grapevine and other fruit cultivars and albeit biocontrol agents are available, superior technologies must be developed to rival synthetic counterparts. In this work, we describe the preparation of grape stalk lipophilic extracts from Malbec, Cabernet sauvignon, Chardonnay and Pedro Giménez as biocontrol tools against Aspergillus candidus, Penicillium chrysogenum, and Rhizopus sp., which are pathogenic for grapes and other fruits. Grape stalk extracts were obtained with hexane, diethyl ether, ethyl acetate, and ethanol. Yields were in the range of 1 to 23% w/w according to solvent polarity. Extracts were separated into lipophilic sub-extracts—yields varied from 70 up to 12% w/w due to solvent polarity. Sub-extracts were assayed for antifungal activity in vitro and the one obtained from Pedro Giménez with ethanol exhibited the broadest biocontrol spectrum at 2.5 × 104 mg/L. This sub-extract was separated into components by column chromatography to inquire further on its biocontrol activity. A bioactivity-guided test against Rhizopus sp. revealed that this effect relies on gamma-sitosterol and a biocontrol cut-off value of 5.1 × 103 mg/L was estimated. EEA San Pedro Fil: Palazzolo, Martín A. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Tecnología Química (INTEQUI); Argentina Fil: Palazzolo, Martín A. Universidad Nacional de San Luis. Instituto de Investigaciones en Tecnología Química (INTEQUI; Argentina Fil: Aballay, Maximiliano Martín. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina Fil: Aballay, Maximiliano Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Tecnología Química (INTEQUI); Argentina Fil: Aballay, Maximiliano Martín. Universidad Nacional de San Luis. Instituto de Investigaciones en Tecnología Química (INTEQUI); Argentina Fil: Martínez, Antares A. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Tecnología Química (INTEQUI); Argentina Fil: Martínez, Antares A. Universidad Nacional de San Luis. Instituto de Investigaciones en Tecnología Química (INTEQUI); Argentina Fil: Kurina-Sanz, Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Tecnología Química (INTEQUI); Argentina Fil: Kurina-Sanz, Marcela. Universidad Nacional de San Luis. Instituto de Investigaciones en Tecnología Química (INTEQUI); Argentina 2023-07-12T10:03:56Z 2023-07-12T10:03:56Z 2022 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/14729 https://link.springer.com/article/10.1007/s12649-021-01533-8 1877-265X 1877-2641 https://doi.org/10.1007/s12649-021-01533-8 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Waste and Biomass Valorization 13 (1) : 609–616 (2022) |
| spellingShingle | Desechos Agrícolas Propiedades Antimicosicas Control Biológico Uva Bioplaguicidas Control de Enfermedades de Plantas Agricultural Wastes Antifungal Properties Biological Control Grapes Biopesticides Plant Disease Control Grape Stalks Plant Sterol Escobajo Esterol Vegetal Raquis de Uva Palazzolo, Martín A. Aballay, Maximiliano Martín Martínez, Antares A. Kurina-Sanz, Marcela Grape Stalk-Based Extracts Controlling Fruit Pathogenic Fungi as a Waste Biomass Valorization Alternative in Winemaking |
| title | Grape Stalk-Based Extracts Controlling Fruit Pathogenic Fungi as a Waste Biomass Valorization Alternative in Winemaking |
| title_full | Grape Stalk-Based Extracts Controlling Fruit Pathogenic Fungi as a Waste Biomass Valorization Alternative in Winemaking |
| title_fullStr | Grape Stalk-Based Extracts Controlling Fruit Pathogenic Fungi as a Waste Biomass Valorization Alternative in Winemaking |
| title_full_unstemmed | Grape Stalk-Based Extracts Controlling Fruit Pathogenic Fungi as a Waste Biomass Valorization Alternative in Winemaking |
| title_short | Grape Stalk-Based Extracts Controlling Fruit Pathogenic Fungi as a Waste Biomass Valorization Alternative in Winemaking |
| title_sort | grape stalk based extracts controlling fruit pathogenic fungi as a waste biomass valorization alternative in winemaking |
| topic | Desechos Agrícolas Propiedades Antimicosicas Control Biológico Uva Bioplaguicidas Control de Enfermedades de Plantas Agricultural Wastes Antifungal Properties Biological Control Grapes Biopesticides Plant Disease Control Grape Stalks Plant Sterol Escobajo Esterol Vegetal Raquis de Uva |
| url | http://hdl.handle.net/20.500.12123/14729 https://link.springer.com/article/10.1007/s12649-021-01533-8 https://doi.org/10.1007/s12649-021-01533-8 |
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