Development and evaluation of a blocking ELISA for serological diagnosis of equine infectious anemia
Equine infectious anemia (EIA) is an important viral disease characterized by persistent infection in equids worldwide. Most EIA cases are life-long virus carriers with low antibody reactions and without the appearance of clinical symptoms. A serological test with high sensitivity and specificity is...
| Autores principales: | , , , , , , , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2023
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/14713 https://link.springer.com/article/10.1007/s00253-023-12504-5 https://doi.org/10.1007/s00253-023-12504-5 |
| _version_ | 1855037169353097216 |
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| author | Zhe, Hu Kui, Guo Cheng, Du Jinhui, Sun Naletoski, Ivancho Xiaoyu, Chu Yuezhi, Lin Xuefeng, Wang Barrandeguy, Maria Edith Miño, Samuel Wen, Wang Lau, Patrick Imtung Wernery, Ulrich Raghavan, Rekha Xiaojun, Wang |
| author_browse | Barrandeguy, Maria Edith Cheng, Du Jinhui, Sun Kui, Guo Lau, Patrick Imtung Miño, Samuel Naletoski, Ivancho Raghavan, Rekha Wen, Wang Wernery, Ulrich Xiaojun, Wang Xiaoyu, Chu Xuefeng, Wang Yuezhi, Lin Zhe, Hu |
| author_facet | Zhe, Hu Kui, Guo Cheng, Du Jinhui, Sun Naletoski, Ivancho Xiaoyu, Chu Yuezhi, Lin Xuefeng, Wang Barrandeguy, Maria Edith Miño, Samuel Wen, Wang Lau, Patrick Imtung Wernery, Ulrich Raghavan, Rekha Xiaojun, Wang |
| author_sort | Zhe, Hu |
| collection | INTA Digital |
| description | Equine infectious anemia (EIA) is an important viral disease characterized by persistent infection in equids worldwide. Most EIA cases are life-long virus carriers with low antibody reactions and without the appearance of clinical symptoms. A serological test with high sensitivity and specificity is required to detect inapparent infection. In this study, a B-cell common epitope-based blocking ELISA (bELISA) was developed using a monoclonal antibody together with the EIAV p26 protein labelled with HRP. The test has been evaluated against the standard and with field serum samples globally. This bELISA test can be completed within 75 min, and the sensitivity is higher than those of either the AGID or one commercial cELISA kit. This bELISA assay was 8–16 times more analytically sensitive than AGID, and 2 to 4 times more analytically sensitive than one cELISA kit by testing three sera from the USA, Argentina, and China, respectively. The 353 serum samples from Argentina were tested, in comparison with AGID, the diagnostic sensitivity and specificity of our bELISA assay were 100% (154/154) and 97.0% (193/199), respectively, and the accuracy of the bELISA test was 98.3%. The bELISA test developed in this study is a rapid, sensitive, specific method for the detection of EIAV infection, and could be a promising candidate for use in the monitoring of the EIA epidemic worldwide. |
| format | info:ar-repo/semantics/artículo |
| id | INTA14713 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA147132023-07-07T13:05:50Z Development and evaluation of a blocking ELISA for serological diagnosis of equine infectious anemia Zhe, Hu Kui, Guo Cheng, Du Jinhui, Sun Naletoski, Ivancho Xiaoyu, Chu Yuezhi, Lin Xuefeng, Wang Barrandeguy, Maria Edith Miño, Samuel Wen, Wang Lau, Patrick Imtung Wernery, Ulrich Raghavan, Rekha Xiaojun, Wang Enfermedades de los Animales Anemia Infecciosa Equina Inmunodiagnóstico Animal Diseases Equine Infectious Anaemia Immunodiagnosis ELISA Equine infectious anemia (EIA) is an important viral disease characterized by persistent infection in equids worldwide. Most EIA cases are life-long virus carriers with low antibody reactions and without the appearance of clinical symptoms. A serological test with high sensitivity and specificity is required to detect inapparent infection. In this study, a B-cell common epitope-based blocking ELISA (bELISA) was developed using a monoclonal antibody together with the EIAV p26 protein labelled with HRP. The test has been evaluated against the standard and with field serum samples globally. This bELISA test can be completed within 75 min, and the sensitivity is higher than those of either the AGID or one commercial cELISA kit. This bELISA assay was 8–16 times more analytically sensitive than AGID, and 2 to 4 times more analytically sensitive than one cELISA kit by testing three sera from the USA, Argentina, and China, respectively. The 353 serum samples from Argentina were tested, in comparison with AGID, the diagnostic sensitivity and specificity of our bELISA assay were 100% (154/154) and 97.0% (193/199), respectively, and the accuracy of the bELISA test was 98.3%. The bELISA test developed in this study is a rapid, sensitive, specific method for the detection of EIAV infection, and could be a promising candidate for use in the monitoring of the EIA epidemic worldwide. Instituto de Virologia Fil: Zhe, Hu. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. State Key Laboratory of Veterinary Biotechnology; China Fil: Zhe, Hu. WOAH Reference Laboratory for Equine Infectious Anemia; China Fil: Kui, Guo. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. State Key Laboratory of Veterinary Biotechnology; China Fil: Cheng, Du. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. State Key Laboratory of Veterinary Biotechnology; China Fil: Jinhui, Sun. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. State Key Laboratory of Veterinary Biotechnology; China Fil: Jinhui, Sun. Northeast Agricultural University; China Fil: Naletoski, Ivancho. International Atomic Energy Agency. Animal Production and Health Section. Joint FAO/IAEA Centre for Nuclear Applications in Food and Agriculture, Department of Nuclear Sciences and Applications; Austria Fil: Xiaoyu, Chu. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. State Key Laboratory of Veterinary Biotechnology; China Fil: Yuezhi, Lin. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. State Key Laboratory of Veterinary Biotechnology; China Fil: Xuefeng, Wang. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. State Key Laboratory of Veterinary Biotechnology; China Fil: Barrandeguy, Maria Edith. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Barrandeguy, Maria Edith. Universidad del Salvador. Facultad de Ciencias Agrarias y Veterinarias; Argentina Fil: Miño, Samuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Wen, Wang. Animal Health Supervision Institute of Xinjiang Uygur Autonomous Region; China Fil: Lau, Patrick Imtung. Agriculture Fisheries and Conservation Department. Equine Disease Division; China Fil: Wernery, Ulrich. Central Veterinary Research Laboratory; Emiratos Arabes Unidos Fil: Raghavan, Rekha. Central Veterinary Research Laboratory; Emiratos Arabes Unidos Fil: Xiaojun, Wang. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. State Key Laboratory of Veterinary Biotechnology; China Fil: Xiaojun, Wang. WOAH Reference Laboratory for Equine Infectious Anemia; China 2023-07-07T12:49:32Z 2023-07-07T12:49:32Z 2023-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/14713 https://link.springer.com/article/10.1007/s00253-023-12504-5 0175-7598 1432-0614 https://doi.org/10.1007/s00253-023-12504-5 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Applied Microbiology and Biotechnology 107 : 3305–3317. (2023) |
| spellingShingle | Enfermedades de los Animales Anemia Infecciosa Equina Inmunodiagnóstico Animal Diseases Equine Infectious Anaemia Immunodiagnosis ELISA Zhe, Hu Kui, Guo Cheng, Du Jinhui, Sun Naletoski, Ivancho Xiaoyu, Chu Yuezhi, Lin Xuefeng, Wang Barrandeguy, Maria Edith Miño, Samuel Wen, Wang Lau, Patrick Imtung Wernery, Ulrich Raghavan, Rekha Xiaojun, Wang Development and evaluation of a blocking ELISA for serological diagnosis of equine infectious anemia |
| title | Development and evaluation of a blocking ELISA for serological diagnosis of equine infectious anemia |
| title_full | Development and evaluation of a blocking ELISA for serological diagnosis of equine infectious anemia |
| title_fullStr | Development and evaluation of a blocking ELISA for serological diagnosis of equine infectious anemia |
| title_full_unstemmed | Development and evaluation of a blocking ELISA for serological diagnosis of equine infectious anemia |
| title_short | Development and evaluation of a blocking ELISA for serological diagnosis of equine infectious anemia |
| title_sort | development and evaluation of a blocking elisa for serological diagnosis of equine infectious anemia |
| topic | Enfermedades de los Animales Anemia Infecciosa Equina Inmunodiagnóstico Animal Diseases Equine Infectious Anaemia Immunodiagnosis ELISA |
| url | http://hdl.handle.net/20.500.12123/14713 https://link.springer.com/article/10.1007/s00253-023-12504-5 https://doi.org/10.1007/s00253-023-12504-5 |
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