South American H4N2 influenza A virus improved replication in chicken trachea after low number of passages
Introduction of influenza A viruses (FLUAV) into poultry from waterfowl is frequent, producing economic burden and increasing the probability of human infections. We have previously described the presence of FLUAV in wild birds in Argentina with unique evolutionary trajectories belonging to a South...
| Autores principales: | , , , , , |
|---|---|
| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Frontiers Media
2023
|
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/14704 https://www.frontiersin.org/articles/10.3389/fvets.2023.1182550/full https://doi.org/10.3389/fvets.2023.1182550 |
| _version_ | 1855485414709657600 |
|---|---|
| author | Ferreri, Lucas Matias Carnaccini, Silvia Olivera, Valeria Soledad Pereda, Ariel Julian Rajao, Daniela Perez, Daniel R. |
| author_browse | Carnaccini, Silvia Ferreri, Lucas Matias Olivera, Valeria Soledad Pereda, Ariel Julian Perez, Daniel R. Rajao, Daniela |
| author_facet | Ferreri, Lucas Matias Carnaccini, Silvia Olivera, Valeria Soledad Pereda, Ariel Julian Rajao, Daniela Perez, Daniel R. |
| author_sort | Ferreri, Lucas Matias |
| collection | INTA Digital |
| description | Introduction of influenza A viruses (FLUAV) into poultry from waterfowl is frequent, producing economic burden and increasing the probability of human infections. We have previously described the presence of FLUAV in wild birds in Argentina with unique evolutionary trajectories belonging to a South American lineage different from the North American and Eurasian lineages. Adaptability of this South American lineage FLUAV to poultry species is still poorly understood. In the present report, we evaluated the capacity of an H4N2 FLUAV from the South American lineage to adapt to chickens after low number of passages. We found that five mutations were acquired after five passages in 3-days-old chickens. These mutations produced a virus with better infectivity in ex vivo trachea explants but overall lower infection in lung explants. Infection of 3-week-old chickens persisted for a longer period and was detected in more tissues than the parental virus, suggesting adaptation of the H4N2 influenza A virus to chicken. |
| format | Artículo |
| id | INTA14704 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | INTA147042023-07-05T18:46:33Z South American H4N2 influenza A virus improved replication in chicken trachea after low number of passages Ferreri, Lucas Matias Carnaccini, Silvia Olivera, Valeria Soledad Pereda, Ariel Julian Rajao, Daniela Perez, Daniel R. Pollo Virus de la Influenza Aviar Aves de Corral Aves Acuáticas Enfermedades de los Animales Chickens Avian Influenzavirus Poultry Waterfowl Animal Diseases South America América del Sur Introduction of influenza A viruses (FLUAV) into poultry from waterfowl is frequent, producing economic burden and increasing the probability of human infections. We have previously described the presence of FLUAV in wild birds in Argentina with unique evolutionary trajectories belonging to a South American lineage different from the North American and Eurasian lineages. Adaptability of this South American lineage FLUAV to poultry species is still poorly understood. In the present report, we evaluated the capacity of an H4N2 FLUAV from the South American lineage to adapt to chickens after low number of passages. We found that five mutations were acquired after five passages in 3-days-old chickens. These mutations produced a virus with better infectivity in ex vivo trachea explants but overall lower infection in lung explants. Infection of 3-week-old chickens persisted for a longer period and was detected in more tissues than the parental virus, suggesting adaptation of the H4N2 influenza A virus to chicken. Instituto de Virología Fil: Ferreri, Lucas. University of Georgia. College of Veterinary Medicine. Poultry Diagnostic and Research Center; Estados Unidos Fil: Ferreri, Lucas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Carnaccini, Silvia. University of Georgia. College of Veterinary Medicine. Poultry Diagnostic and Research Center; Estados Unidos Fil: Olivera, Valeria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Pereda, Ariel Julian. Instituto Nacional de Tecnología Agropecuaria (INTA). Programa Nacional Salud Animal; Argentina Fil: Rajao, Daniela. University of Georgia. College of Veterinary Medicine. Poultry Diagnostic and Research Center; Estados Unidos Fil: Perez, Daniel R. University of Georgia. College of Veterinary Medicine. Poultry Diagnostic and Research Center; Estados Unidos 2023-07-05T18:32:31Z 2023-07-05T18:32:31Z 2023-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/14704 https://www.frontiersin.org/articles/10.3389/fvets.2023.1182550/full 2297-1769 https://doi.org/10.3389/fvets.2023.1182550 eng info:eu-repograntAgreement/INTA/PNSA-1115052/AR./Epidemiología y desarrollo de estrategias para la prevención y control de enfermedades que afectan la salud pública, enfermedades exóticas y limitantes del comercio internacional. info:eu-repograntAgreement/INTA/PNSA-1115056/AR./Enfermedades infecciosas de las aves. info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf South America .......... (continent) (World) 1000002 Frontiers Media Frontiers in Veterinary Science 10 : 1182550. (May 2023) |
| spellingShingle | Pollo Virus de la Influenza Aviar Aves de Corral Aves Acuáticas Enfermedades de los Animales Chickens Avian Influenzavirus Poultry Waterfowl Animal Diseases South America América del Sur Ferreri, Lucas Matias Carnaccini, Silvia Olivera, Valeria Soledad Pereda, Ariel Julian Rajao, Daniela Perez, Daniel R. South American H4N2 influenza A virus improved replication in chicken trachea after low number of passages |
| title | South American H4N2 influenza A virus improved replication in chicken trachea after low number of passages |
| title_full | South American H4N2 influenza A virus improved replication in chicken trachea after low number of passages |
| title_fullStr | South American H4N2 influenza A virus improved replication in chicken trachea after low number of passages |
| title_full_unstemmed | South American H4N2 influenza A virus improved replication in chicken trachea after low number of passages |
| title_short | South American H4N2 influenza A virus improved replication in chicken trachea after low number of passages |
| title_sort | south american h4n2 influenza a virus improved replication in chicken trachea after low number of passages |
| topic | Pollo Virus de la Influenza Aviar Aves de Corral Aves Acuáticas Enfermedades de los Animales Chickens Avian Influenzavirus Poultry Waterfowl Animal Diseases South America América del Sur |
| url | http://hdl.handle.net/20.500.12123/14704 https://www.frontiersin.org/articles/10.3389/fvets.2023.1182550/full https://doi.org/10.3389/fvets.2023.1182550 |
| work_keys_str_mv | AT ferrerilucasmatias southamericanh4n2influenzaavirusimprovedreplicationinchickentracheaafterlownumberofpassages AT carnaccinisilvia southamericanh4n2influenzaavirusimprovedreplicationinchickentracheaafterlownumberofpassages AT oliveravaleriasoledad southamericanh4n2influenzaavirusimprovedreplicationinchickentracheaafterlownumberofpassages AT peredaarieljulian southamericanh4n2influenzaavirusimprovedreplicationinchickentracheaafterlownumberofpassages AT rajaodaniela southamericanh4n2influenzaavirusimprovedreplicationinchickentracheaafterlownumberofpassages AT perezdanielr southamericanh4n2influenzaavirusimprovedreplicationinchickentracheaafterlownumberofpassages |