Serological screening of SARS-CoV-2 infection in companion animals of Buenos Aires suburbs
The Coronavirus Disease 2019 (COVID-19) is a zoonotic disease caused by the pandemic virus SARS-CoV-2. Domestic and wild animals are susceptible to infection and are potential reservoirs for virus variants. To date, there is no information about the exposure of companion animals in Buenos Aires Subu...
| Main Authors: | , , , , , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
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Frontiers Media
2023
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/14703 https://www.frontiersin.org/articles/10.3389/fvets.2023.1161820/full https://doi.org/10.3389/fvets.2023.1161820 |
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| author | Cardoso, Nancy Rivero, Carla Castillo, Mariangeles Mansilla, Florencia Celeste Pastorino, Florencia Piccirilli, Guadalupe Alonso, Laura Martinez, Gustavo Di Lullo, David Bentancor, Leticia Veronica Capozzo, Alejandra |
| author_browse | Alonso, Laura Bentancor, Leticia Veronica Capozzo, Alejandra Cardoso, Nancy Castillo, Mariangeles Di Lullo, David Mansilla, Florencia Celeste Martinez, Gustavo Pastorino, Florencia Piccirilli, Guadalupe Rivero, Carla |
| author_facet | Cardoso, Nancy Rivero, Carla Castillo, Mariangeles Mansilla, Florencia Celeste Pastorino, Florencia Piccirilli, Guadalupe Alonso, Laura Martinez, Gustavo Di Lullo, David Bentancor, Leticia Veronica Capozzo, Alejandra |
| author_sort | Cardoso, Nancy |
| collection | INTA Digital |
| description | The Coronavirus Disease 2019 (COVID-19) is a zoonotic disease caused by the pandemic virus SARS-CoV-2. Domestic and wild animals are susceptible to infection and are potential reservoirs for virus variants. To date, there is no information about the exposure of companion animals in Buenos Aires Suburbs, the area with the largest population in Argentina where the highest number of COVID-19 human cases occurred during the first infection wave. Here we developed a multi-species indirect ELISA to measure antibodies reactive to the SARS-CoV-2 receptor-binding domain (RBD) from several vertebrates constituting the class Mammalia, making it a valuable tool for field serosurveillance. The ELISA cut-off value was estimated by sera from dogs, cats, cattle, and pigs sampled before 2019 (n = 170), considering a 98% percentile and a grey zone to completely exclude any false positive result. Specificity was confirmed by measuring levels of neutralizing antibodies against canine coronavirus, the avidity of specific antibodies, and their capacity to impede the binding of a recombinant RBD protein to VERO cells in an In-Cell ELISA. Sera from 464 cats and dogs sampled in 2020 and 2021 (“pandemic” samples) were assessed using the RBD-ELISA. Information on COVID-19 disease in the household and the animals’ lifestyles was collected. In Buenos Aires Suburbs cats were infected at a higher proportion than dogs, seroprevalence was 7.1 and 1.68%, respectively. Confirmed COVID-19 in the caregivers and outdoor lifestyle were statistically associated with seropositivity in cats. The risk of cats getting infected living indoors in COVID-19-negative households was null. The susceptibility of mammals to SARS-CoV-2, the possibility of transmission between animals themselves and humans, together with the free-roaming lifestyle typical of Buenos Aires suburban companion animals, urge pursuing responsible animal care and avoiding human interaction with animals during the disease course. The multi-species RBD-ELISA we developed can be used as a tool for serosurveillance of SARS-CoV-2 infection in mammalians (domestic and wild), guiding further targeted virological analyses to encounter susceptible species, interspecies transmission, and potential virus reservoirs in our region. |
| format | Artículo |
| id | INTA14703 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | INTA147032023-07-05T17:49:22Z Serological screening of SARS-CoV-2 infection in companion animals of Buenos Aires suburbs Cardoso, Nancy Rivero, Carla Castillo, Mariangeles Mansilla, Florencia Celeste Pastorino, Florencia Piccirilli, Guadalupe Alonso, Laura Martinez, Gustavo Di Lullo, David Bentancor, Leticia Veronica Capozzo, Alejandra COVID-19 Severe Acute Respiratory Syndrome Coronavirus 2 Domestic Animals Immunodiagnosis ELISA Screening Coronavirus del Síndrome Respiratorio Agudo Grave 2 Animales Domésticos Inmunodiagnóstico Evaluación Preliminar SARS-CoV-2 Suburbios de Buenos Aires The Coronavirus Disease 2019 (COVID-19) is a zoonotic disease caused by the pandemic virus SARS-CoV-2. Domestic and wild animals are susceptible to infection and are potential reservoirs for virus variants. To date, there is no information about the exposure of companion animals in Buenos Aires Suburbs, the area with the largest population in Argentina where the highest number of COVID-19 human cases occurred during the first infection wave. Here we developed a multi-species indirect ELISA to measure antibodies reactive to the SARS-CoV-2 receptor-binding domain (RBD) from several vertebrates constituting the class Mammalia, making it a valuable tool for field serosurveillance. The ELISA cut-off value was estimated by sera from dogs, cats, cattle, and pigs sampled before 2019 (n = 170), considering a 98% percentile and a grey zone to completely exclude any false positive result. Specificity was confirmed by measuring levels of neutralizing antibodies against canine coronavirus, the avidity of specific antibodies, and their capacity to impede the binding of a recombinant RBD protein to VERO cells in an In-Cell ELISA. Sera from 464 cats and dogs sampled in 2020 and 2021 (“pandemic” samples) were assessed using the RBD-ELISA. Information on COVID-19 disease in the household and the animals’ lifestyles was collected. In Buenos Aires Suburbs cats were infected at a higher proportion than dogs, seroprevalence was 7.1 and 1.68%, respectively. Confirmed COVID-19 in the caregivers and outdoor lifestyle were statistically associated with seropositivity in cats. The risk of cats getting infected living indoors in COVID-19-negative households was null. The susceptibility of mammals to SARS-CoV-2, the possibility of transmission between animals themselves and humans, together with the free-roaming lifestyle typical of Buenos Aires suburban companion animals, urge pursuing responsible animal care and avoiding human interaction with animals during the disease course. The multi-species RBD-ELISA we developed can be used as a tool for serosurveillance of SARS-CoV-2 infection in mammalians (domestic and wild), guiding further targeted virological analyses to encounter susceptible species, interspecies transmission, and potential virus reservoirs in our region. Instituto de Virología Fil: Cardoso, Nancy Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Cardoso, Nancy Patricia. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina Fil: Rivero, Carla. Universidad Nacional de José Clemente Paz. Instituto de Estudios para el Desarrollo Productivo y la Innovación; Argentina Fil: Castillo, Mariangeles. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Castillo, Mariangeles. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina Fil: Mansilla, Florencia Celeste. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Fil: Pastorino, Florencia. Provincia de Buenos Aires. Ministerio de Salud. Departamento Zoonosis Urbanas; Argentina Fil: Piccirilli, Guadalupe. Provincia de Buenos Aires. Ministerio de Salud. Departamento Zoonosis Urbanas; Argentina Fil: Alonso, Maria Laura. Provincia de Buenos Aires. Ministerio de Salud. Departamento Zoonosis Urbanas; Argentina Fil: Martinez, Gustavo. Provincia de Buenos Aires. Ministerio de Salud. Departamento Zoonosis Urbanas; Argentina Fil: Di Lullo, David. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Salud, Tecnología y Desarrollo; Argentina Fil: Di Lullo, David. Universidad Nacional de Santiago del Estero. Instituto Multidisciplinario de Salud, Tecnología y Desarrollo; Argentina Fil: Bentancor, Leticia Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bentancor, Leticia Veronica. Universidad Nacional de José Clemente Paz. Instituto de Estudios para el Desarrollo Productivo y la Innovación; Argentina Fil: Capozzo, Alejandra Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Capozzo, Alejandra Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2023-07-05T17:42:07Z 2023-07-05T17:42:07Z 2023-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/14703 https://www.frontiersin.org/articles/10.3389/fvets.2023.1161820/full 2297-1769 https://doi.org/10.3389/fvets.2023.1161820 eng info:eu-repograntAgreement/INTA/2019-PD-E5-I105-001/2019-PD-E5-I105-001, Patógenos animales: su interacción con el hospedador y el medio ambiente. Impacto en productividad, ecosistemas, sanidad animal y salud pública en el marco ?Una Salud? info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Greater Buenos Aires .......... (general region) (World, South America, Argentina) 8725264 Frontiers Media Frontiers in Veterinary Science 10 : 1161820. (May 2023) |
| spellingShingle | COVID-19 Severe Acute Respiratory Syndrome Coronavirus 2 Domestic Animals Immunodiagnosis ELISA Screening Coronavirus del Síndrome Respiratorio Agudo Grave 2 Animales Domésticos Inmunodiagnóstico Evaluación Preliminar SARS-CoV-2 Suburbios de Buenos Aires Cardoso, Nancy Rivero, Carla Castillo, Mariangeles Mansilla, Florencia Celeste Pastorino, Florencia Piccirilli, Guadalupe Alonso, Laura Martinez, Gustavo Di Lullo, David Bentancor, Leticia Veronica Capozzo, Alejandra Serological screening of SARS-CoV-2 infection in companion animals of Buenos Aires suburbs |
| title | Serological screening of SARS-CoV-2 infection in companion animals of Buenos Aires suburbs |
| title_full | Serological screening of SARS-CoV-2 infection in companion animals of Buenos Aires suburbs |
| title_fullStr | Serological screening of SARS-CoV-2 infection in companion animals of Buenos Aires suburbs |
| title_full_unstemmed | Serological screening of SARS-CoV-2 infection in companion animals of Buenos Aires suburbs |
| title_short | Serological screening of SARS-CoV-2 infection in companion animals of Buenos Aires suburbs |
| title_sort | serological screening of sars cov 2 infection in companion animals of buenos aires suburbs |
| topic | COVID-19 Severe Acute Respiratory Syndrome Coronavirus 2 Domestic Animals Immunodiagnosis ELISA Screening Coronavirus del Síndrome Respiratorio Agudo Grave 2 Animales Domésticos Inmunodiagnóstico Evaluación Preliminar SARS-CoV-2 Suburbios de Buenos Aires |
| url | http://hdl.handle.net/20.500.12123/14703 https://www.frontiersin.org/articles/10.3389/fvets.2023.1161820/full https://doi.org/10.3389/fvets.2023.1161820 |
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