The Immune Response in the Uteri and Placentae of Chlamydia abortus-Infected Ewes and Its Association with Pregnancy Outcomes
The enzootic abortion of ewes, caused by the bacterium Chlamydia abortus (C. abortus), is one of the main causes of abortion in sheep. There are multiple contributory factors, including chlamydial growth, host immune response, and hormonal balance, that result in different pregnancy outcomes, such a...
| Autores principales: | , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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MDPI
2023
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/14680 https://www.mdpi.com/2076-0817/12/6/846 https://doi.org/10.3390/pathogens12060846 |
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| author | Caspe, Sergio Gaston Ewing, David Andrew Livingstone, Morag Underwood, Clare Milne, Elspeth Sargison, Neil Donald Wattegedera, Sean Ranjan Longbottom, David |
| author_browse | Caspe, Sergio Gaston Ewing, David Andrew Livingstone, Morag Longbottom, David Milne, Elspeth Sargison, Neil Donald Underwood, Clare Wattegedera, Sean Ranjan |
| author_facet | Caspe, Sergio Gaston Ewing, David Andrew Livingstone, Morag Underwood, Clare Milne, Elspeth Sargison, Neil Donald Wattegedera, Sean Ranjan Longbottom, David |
| author_sort | Caspe, Sergio Gaston |
| collection | INTA Digital |
| description | The enzootic abortion of ewes, caused by the bacterium Chlamydia abortus (C. abortus), is one of the main causes of abortion in sheep. There are multiple contributory factors, including chlamydial growth, host immune response, and hormonal balance, that result in different pregnancy outcomes, such as abortion, the birth of weak lambs that may die, or healthy lambs. This study aimed to determine the relationship between phenotypical patterns of immune cell infiltration and different pregnancy outcomes in twin-bearing sheep (both lambs born dead; one alive and one dead; both alive) when experimentally infected with C. abortus. Both the sheep uteri and placentae were collected after parturition. All samples were analysed for specific immune cell features, including cell surface antigens and the T-regulatory (Treg) cell-associated transcription factor and cytokines, by immunohistochemistry and in situ hybridisation. Some of these immunological antigens were evaluated in ovine reproductive tissues for the first time. Differential patterns of T helper/Treg cells revealed significant group effects in the placentae. It suggests the potential role that the balance of lymphocyte subsets may play in affecting different pregnancy outcomes in C. abortus-infected sheep. The present study provides novel detailed information about the immune responses observed at the maternofoetal interface in sheep at the time of pre-term abortion or lambing. |
| format | info:ar-repo/semantics/artículo |
| id | INTA14680 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INTA146802023-07-03T11:33:09Z The Immune Response in the Uteri and Placentae of Chlamydia abortus-Infected Ewes and Its Association with Pregnancy Outcomes Caspe, Sergio Gaston Ewing, David Andrew Livingstone, Morag Underwood, Clare Milne, Elspeth Sargison, Neil Donald Wattegedera, Sean Ranjan Longbottom, David Oveja Enfermedades de los Animales Aborto Respuesta Inmunológica Ewes Chlamydophila abortus Animal Diseases Abortion Placenta Immune Response Chlamydia abortus The enzootic abortion of ewes, caused by the bacterium Chlamydia abortus (C. abortus), is one of the main causes of abortion in sheep. There are multiple contributory factors, including chlamydial growth, host immune response, and hormonal balance, that result in different pregnancy outcomes, such as abortion, the birth of weak lambs that may die, or healthy lambs. This study aimed to determine the relationship between phenotypical patterns of immune cell infiltration and different pregnancy outcomes in twin-bearing sheep (both lambs born dead; one alive and one dead; both alive) when experimentally infected with C. abortus. Both the sheep uteri and placentae were collected after parturition. All samples were analysed for specific immune cell features, including cell surface antigens and the T-regulatory (Treg) cell-associated transcription factor and cytokines, by immunohistochemistry and in situ hybridisation. Some of these immunological antigens were evaluated in ovine reproductive tissues for the first time. Differential patterns of T helper/Treg cells revealed significant group effects in the placentae. It suggests the potential role that the balance of lymphocyte subsets may play in affecting different pregnancy outcomes in C. abortus-infected sheep. The present study provides novel detailed information about the immune responses observed at the maternofoetal interface in sheep at the time of pre-term abortion or lambing. EEA Mercedes Fil: Caspe, Sergio Gaston. Moredun Research Institute; Reino Unido Fil: Caspe, Sergio Gaston. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina Fil: Ewing, David Andrew. Biomathematics & Statistics Scotland; Reino Unido Fil: Livingstone, Morag. Moredun Research Institute; Reino Unido Fil: Underwood, Clare. Moredun Research Institute; Reino Unido Fil: Milne, Elspeth. University of Edinburgh. Royal (Dick) School of Veterinary Studies; Reino Unido Fil: Sargison, Neil Donald. University of Edinburgh. Royal (Dick) School of Veterinary Studies; Reino Unido Fil: Wattegedera, Sean Ranjan. Moredun Research Institute; Reino Unido Fil: Longbottom, David. Moredun Research Institute; Reino Unido 2023-07-03T11:24:52Z 2023-07-03T11:24:52Z 2023-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/14680 https://www.mdpi.com/2076-0817/12/6/846 2076-0817 https://doi.org/10.3390/pathogens12060846 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Pathogens 12 (6) : 846. (June 2023) |
| spellingShingle | Oveja Enfermedades de los Animales Aborto Respuesta Inmunológica Ewes Chlamydophila abortus Animal Diseases Abortion Placenta Immune Response Chlamydia abortus Caspe, Sergio Gaston Ewing, David Andrew Livingstone, Morag Underwood, Clare Milne, Elspeth Sargison, Neil Donald Wattegedera, Sean Ranjan Longbottom, David The Immune Response in the Uteri and Placentae of Chlamydia abortus-Infected Ewes and Its Association with Pregnancy Outcomes |
| title | The Immune Response in the Uteri and Placentae of Chlamydia abortus-Infected Ewes and Its Association with Pregnancy Outcomes |
| title_full | The Immune Response in the Uteri and Placentae of Chlamydia abortus-Infected Ewes and Its Association with Pregnancy Outcomes |
| title_fullStr | The Immune Response in the Uteri and Placentae of Chlamydia abortus-Infected Ewes and Its Association with Pregnancy Outcomes |
| title_full_unstemmed | The Immune Response in the Uteri and Placentae of Chlamydia abortus-Infected Ewes and Its Association with Pregnancy Outcomes |
| title_short | The Immune Response in the Uteri and Placentae of Chlamydia abortus-Infected Ewes and Its Association with Pregnancy Outcomes |
| title_sort | immune response in the uteri and placentae of chlamydia abortus infected ewes and its association with pregnancy outcomes |
| topic | Oveja Enfermedades de los Animales Aborto Respuesta Inmunológica Ewes Chlamydophila abortus Animal Diseases Abortion Placenta Immune Response Chlamydia abortus |
| url | http://hdl.handle.net/20.500.12123/14680 https://www.mdpi.com/2076-0817/12/6/846 https://doi.org/10.3390/pathogens12060846 |
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