Characterisation and transferability of transcriptomic microsatellite markers for Nothofagus species
Discriminant molecular markers are required for research on population genetics, as well as evolutionary studies involving identification of hybrids and parental species, or detection of the genome regions under selection. We provide a set of 27 transcriptomic microsatellite markers (SSRs) for South...
| Autores principales: | , , , , , |
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| Formato: | info:eu-repo/semantics/article |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/1468 http://www.tandfonline.com/doi/abs/10.1080/0028825X.2017.1340310?journalCode=tnzb20 http://dx.doi.org/10.1080/0028825X.2017.1340310 |
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| author | El Mujtar, Veronica Andrea Lopez, Micaela Amalfi, Sabrina Pomponio, Maria Florencia Marcucci Poltri, Susana Noemi Torales, Susana Leonor |
| author_browse | Amalfi, Sabrina El Mujtar, Veronica Andrea Lopez, Micaela Marcucci Poltri, Susana Noemi Pomponio, Maria Florencia Torales, Susana Leonor |
| author_facet | El Mujtar, Veronica Andrea Lopez, Micaela Amalfi, Sabrina Pomponio, Maria Florencia Marcucci Poltri, Susana Noemi Torales, Susana Leonor |
| author_sort | El Mujtar, Veronica Andrea |
| collection | INTA Digital |
| description | Discriminant molecular markers are required for research on population genetics, as well as evolutionary studies involving identification of hybrids and parental species, or detection of the genome regions under selection. We provide a set of 27 transcriptomic microsatellite markers (SSRs) for South American Nothofagus species, derived from 73 Nothofagus alpina (=N. nervosa) annotated unigenes. Rates of cross-amplification ranged from 22% to 37%. Genetic characterisation of 22 transcriptomic SSRs for N. alpina and N. obliqua reveals low genetic variability, due to the general occurrence of one major allele at each locus, and high specificity, with few alleles shared between species (14%). At inter-species level 95% of loci were discriminant, with a total G’’st over loci of 0.9, indicating that
alleles were mostly fixed for all loci in both species. At intraspecies level the number of markers with significant differentiation was 2.5 times higher for N. obliqua than for N. alpina populations. Moreover, transcriptomic SSRs showed
higher performance compared with published anonymous microsatellites isolated from genome sequences without annotation. This set of transcriptomic microsatellites will be useful to the scientific community working on conservation and evolutionary aspects of Nothofagus species. |
| format | info:eu-repo/semantics/article |
| id | INTA1468 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA14682018-10-10T11:21:48Z Characterisation and transferability of transcriptomic microsatellite markers for Nothofagus species El Mujtar, Veronica Andrea Lopez, Micaela Amalfi, Sabrina Pomponio, Maria Florencia Marcucci Poltri, Susana Noemi Torales, Susana Leonor Genética Nothofagus Marcadores Genéticos Microsatélites Genetics Genetic Markers Microsatellites Discriminant molecular markers are required for research on population genetics, as well as evolutionary studies involving identification of hybrids and parental species, or detection of the genome regions under selection. We provide a set of 27 transcriptomic microsatellite markers (SSRs) for South American Nothofagus species, derived from 73 Nothofagus alpina (=N. nervosa) annotated unigenes. Rates of cross-amplification ranged from 22% to 37%. Genetic characterisation of 22 transcriptomic SSRs for N. alpina and N. obliqua reveals low genetic variability, due to the general occurrence of one major allele at each locus, and high specificity, with few alleles shared between species (14%). At inter-species level 95% of loci were discriminant, with a total G’’st over loci of 0.9, indicating that alleles were mostly fixed for all loci in both species. At intraspecies level the number of markers with significant differentiation was 2.5 times higher for N. obliqua than for N. alpina populations. Moreover, transcriptomic SSRs showed higher performance compared with published anonymous microsatellites isolated from genome sequences without annotation. This set of transcriptomic microsatellites will be useful to the scientific community working on conservation and evolutionary aspects of Nothofagus species. Inst. de Biotecnología Fil: El Mujtar, Verónica Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimenta Agropecuaria Pergamino. Unidad de Genética Ecológica y Mejoramiento Forestal; Argentina Fil: Lopez, Marta Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina Fil: Amalfi, Sabrina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Pomponio, Maria Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina Fil: Marcucci Poltri, Susana Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Torales, Susana Leonor. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina 2017-10-12T11:36:35Z 2017-10-12T11:36:35Z 2017-07 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion info:ar-repo/semantics/artículo http://hdl.handle.net/20.500.12123/1468 http://www.tandfonline.com/doi/abs/10.1080/0028825X.2017.1340310?journalCode=tnzb20 0028-825X (Print) 1175-8643 (Online) http://dx.doi.org/10.1080/0028825X.2017.1340310 eng info:eu-repo/semantics/restrictedAccess application/pdf New Zealand journal of botany 55 (3) : 1-10. (2017) |
| spellingShingle | Genética Nothofagus Marcadores Genéticos Microsatélites Genetics Genetic Markers Microsatellites El Mujtar, Veronica Andrea Lopez, Micaela Amalfi, Sabrina Pomponio, Maria Florencia Marcucci Poltri, Susana Noemi Torales, Susana Leonor Characterisation and transferability of transcriptomic microsatellite markers for Nothofagus species |
| title | Characterisation and transferability of transcriptomic microsatellite markers for Nothofagus species |
| title_full | Characterisation and transferability of transcriptomic microsatellite markers for Nothofagus species |
| title_fullStr | Characterisation and transferability of transcriptomic microsatellite markers for Nothofagus species |
| title_full_unstemmed | Characterisation and transferability of transcriptomic microsatellite markers for Nothofagus species |
| title_short | Characterisation and transferability of transcriptomic microsatellite markers for Nothofagus species |
| title_sort | characterisation and transferability of transcriptomic microsatellite markers for nothofagus species |
| topic | Genética Nothofagus Marcadores Genéticos Microsatélites Genetics Genetic Markers Microsatellites |
| url | http://hdl.handle.net/20.500.12123/1468 http://www.tandfonline.com/doi/abs/10.1080/0028825X.2017.1340310?journalCode=tnzb20 http://dx.doi.org/10.1080/0028825X.2017.1340310 |
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