Characterization of Purple Carrot Germplasm for Antioxidant Capacity and Root Concentration of Anthocyanins, Phenolics, and Carotenoids

The present study characterized a genetically and phenotypically diverse collection of 27 purple and two non-purple (one orange and one yellow) carrot accessions for concentration of root anthocyanins, phenolics, and carotenoids, and antioxidant capacity estimated by four different methods (ORAC, DP...

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Main Authors: Perez, María Belén, Carvajal, Sofía, Beretta, Vanesa, Bannoud, Florencia, Fangio, María Florencia, Berli, Federico Javier, Fontana, Ariel, Salomón, María Victoria, Gonzalez, Roxana Elizabeth, Valerga, Lucia, Altamirano, Jorgelina C., Yildiz, Mehtap, Iorizzo, Massimo, Simon, Philipp W., Cavagnaro, Pablo
Format: Artículo
Language:Inglés
Published: MDPI 2023
Subjects:
Online Access:http://hdl.handle.net/20.500.12123/14662
https://www.mdpi.com/2223-7747/12/9/1796
https://doi.org/10.3390/plants12091796
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author Perez, María Belén
Carvajal, Sofía
Beretta, Vanesa
Bannoud, Florencia
Fangio, María Florencia
Berli, Federico Javier
Fontana, Ariel
Salomón, María Victoria
Gonzalez, Roxana Elizabeth
Valerga, Lucia
Altamirano, Jorgelina C.
Yildiz, Mehtap
Iorizzo, Massimo
Simon, Philipp W.
Cavagnaro, Pablo
author_browse Altamirano, Jorgelina C.
Bannoud, Florencia
Beretta, Vanesa
Berli, Federico Javier
Carvajal, Sofía
Cavagnaro, Pablo
Fangio, María Florencia
Fontana, Ariel
Gonzalez, Roxana Elizabeth
Iorizzo, Massimo
Perez, María Belén
Salomón, María Victoria
Simon, Philipp W.
Valerga, Lucia
Yildiz, Mehtap
author_facet Perez, María Belén
Carvajal, Sofía
Beretta, Vanesa
Bannoud, Florencia
Fangio, María Florencia
Berli, Federico Javier
Fontana, Ariel
Salomón, María Victoria
Gonzalez, Roxana Elizabeth
Valerga, Lucia
Altamirano, Jorgelina C.
Yildiz, Mehtap
Iorizzo, Massimo
Simon, Philipp W.
Cavagnaro, Pablo
author_sort Perez, María Belén
collection INTA Digital
description The present study characterized a genetically and phenotypically diverse collection of 27 purple and two non-purple (one orange and one yellow) carrot accessions for concentration of root anthocyanins, phenolics, and carotenoids, and antioxidant capacity estimated by four different methods (ORAC, DPPH, ABTS, FRAP), in a partially replicated experimental design comprising data from two growing seasons (2018 and 2019). Broad and significant (p < 0.0001) variation was found among the accessions for all the traits. Acylated anthocyanins (AA) predominated over non-acylated anthocyanins (NAA) in all the accessions and years analyzed, with AA accounting for 55.5–100% of the total anthocyanin content (TAC). Anthocyanins acylated with ferulic acid and coumaric acid were the most abundant carrot anthocyanins. In general, black or solid purple carrots had the greatest TAC and total phenolic content (TPC), and the strongest antioxidant capacities, measured by all methods. Antioxidant capacity, estimated by all methods, was significantly, positively, and moderately-to-strongly correlated with the content of all individual anthocyanins pigments, TAC, and TPC, in both years (r = 0.59–0.90, p < 0.0001), but not with the carotenoid pigments lutein and β-carotene; suggesting that anthocyanins and other phenolics, but not carotenoids, are major contributors of the antioxidant capacity in purple carrots. We identified accessions with high concentration of chemically stable AA, with potential value for the production of food dyes, and accessions with relatively high content of bioavailable NAA that can be selected for increased nutraceutical value (e.g., for fresh consumption).
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spelling INTA146622023-06-28T19:00:04Z Characterization of Purple Carrot Germplasm for Antioxidant Capacity and Root Concentration of Anthocyanins, Phenolics, and Carotenoids Perez, María Belén Carvajal, Sofía Beretta, Vanesa Bannoud, Florencia Fangio, María Florencia Berli, Federico Javier Fontana, Ariel Salomón, María Victoria Gonzalez, Roxana Elizabeth Valerga, Lucia Altamirano, Jorgelina C. Yildiz, Mehtap Iorizzo, Massimo Simon, Philipp W. Cavagnaro, Pablo Zanahoria Variedades Germoplasma Propiedades Antioxidantes Antocianinas Compuestos Fenólicos Carotenoides Carrots Varieties Germplasm Antioxidant Properties Anthocyanins Phenolic Compounds Carotenoids Zanahoria Morada Purple Carrots The present study characterized a genetically and phenotypically diverse collection of 27 purple and two non-purple (one orange and one yellow) carrot accessions for concentration of root anthocyanins, phenolics, and carotenoids, and antioxidant capacity estimated by four different methods (ORAC, DPPH, ABTS, FRAP), in a partially replicated experimental design comprising data from two growing seasons (2018 and 2019). Broad and significant (p < 0.0001) variation was found among the accessions for all the traits. Acylated anthocyanins (AA) predominated over non-acylated anthocyanins (NAA) in all the accessions and years analyzed, with AA accounting for 55.5–100% of the total anthocyanin content (TAC). Anthocyanins acylated with ferulic acid and coumaric acid were the most abundant carrot anthocyanins. In general, black or solid purple carrots had the greatest TAC and total phenolic content (TPC), and the strongest antioxidant capacities, measured by all methods. Antioxidant capacity, estimated by all methods, was significantly, positively, and moderately-to-strongly correlated with the content of all individual anthocyanins pigments, TAC, and TPC, in both years (r = 0.59–0.90, p < 0.0001), but not with the carotenoid pigments lutein and β-carotene; suggesting that anthocyanins and other phenolics, but not carotenoids, are major contributors of the antioxidant capacity in purple carrots. We identified accessions with high concentration of chemically stable AA, with potential value for the production of food dyes, and accessions with relatively high content of bioavailable NAA that can be selected for increased nutraceutical value (e.g., for fresh consumption). EEA La Consulta Fil: Pérez, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Pérez, María Belén. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina Fil: Carvajal, Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Carvajal, Sofía. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina Fil: Beretta, Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bannoud, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Fangio, María Florencia. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química. Instituto de Investigaciones Físicas de Mar del Plata; Argentina Fil: Fangio, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Físicas de Mar del Plata; Argentina Fil: Berli, Federico Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Berli, Federico Javier. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Fontana, Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Fontana, Ariel. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Salomon, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Salomon, María Victoria. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Gonzalez, Roxana Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina Fil: Valerga, Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Valerga, Lucia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina Fil: Altamirano, Jorgelina C. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales; Argentina Fil: Altamirano, Jorgelina C. Universidad Nacional de Cuyo. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales; Argentina Fil: Altamirano, Jorgelina C. Gobierno de Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales; Argentina Fil: Altamirano, Jorgelina C. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Yildiz, Mehtap. Van Yüzüncü Yıl University. Faculty of Agriculture. Department of Agricultural Biotechnology; Turquía Fil: Iorizzo, Massimo. North Carolina State University. Plants for Human Health Institute; Estados Unidos Fil: Iorizzo, Massimo. North Carolina State University. Department of Horticultural Science; Estados Unidos Fil: Simon, Philipp W. University of Wisconsin-Madison. Department of Horticulture; Estados Unidos Fil: Simon, Philipp W. USDA-Agricultural Research Service. Vegetable Crops Research Unit; Estados Unidos Fil: Cavagnaro, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Cavagnaro, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Fil: Cavagnaro, Pablo. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; Argentina 2023-06-27T13:28:08Z 2023-06-27T13:28:08Z 2023-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/14662 https://www.mdpi.com/2223-7747/12/9/1796 2223-7747 https://doi.org/10.3390/plants12091796 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Plants 12 (9) : 1796. (April 2023)
spellingShingle Zanahoria
Variedades
Germoplasma
Propiedades Antioxidantes
Antocianinas
Compuestos Fenólicos
Carotenoides
Carrots
Varieties
Germplasm
Antioxidant Properties
Anthocyanins
Phenolic Compounds
Carotenoids
Zanahoria Morada
Purple Carrots
Perez, María Belén
Carvajal, Sofía
Beretta, Vanesa
Bannoud, Florencia
Fangio, María Florencia
Berli, Federico Javier
Fontana, Ariel
Salomón, María Victoria
Gonzalez, Roxana Elizabeth
Valerga, Lucia
Altamirano, Jorgelina C.
Yildiz, Mehtap
Iorizzo, Massimo
Simon, Philipp W.
Cavagnaro, Pablo
Characterization of Purple Carrot Germplasm for Antioxidant Capacity and Root Concentration of Anthocyanins, Phenolics, and Carotenoids
title Characterization of Purple Carrot Germplasm for Antioxidant Capacity and Root Concentration of Anthocyanins, Phenolics, and Carotenoids
title_full Characterization of Purple Carrot Germplasm for Antioxidant Capacity and Root Concentration of Anthocyanins, Phenolics, and Carotenoids
title_fullStr Characterization of Purple Carrot Germplasm for Antioxidant Capacity and Root Concentration of Anthocyanins, Phenolics, and Carotenoids
title_full_unstemmed Characterization of Purple Carrot Germplasm for Antioxidant Capacity and Root Concentration of Anthocyanins, Phenolics, and Carotenoids
title_short Characterization of Purple Carrot Germplasm for Antioxidant Capacity and Root Concentration of Anthocyanins, Phenolics, and Carotenoids
title_sort characterization of purple carrot germplasm for antioxidant capacity and root concentration of anthocyanins phenolics and carotenoids
topic Zanahoria
Variedades
Germoplasma
Propiedades Antioxidantes
Antocianinas
Compuestos Fenólicos
Carotenoides
Carrots
Varieties
Germplasm
Antioxidant Properties
Anthocyanins
Phenolic Compounds
Carotenoids
Zanahoria Morada
Purple Carrots
url http://hdl.handle.net/20.500.12123/14662
https://www.mdpi.com/2223-7747/12/9/1796
https://doi.org/10.3390/plants12091796
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