An integrative 'omics' approach identifies new candidate genes to impact aroma volatiles in peach fruit

Background: Ever since the recent completion of the peach genome, the focus of genetic research in this area has turned to the identification of genes related to important traits, such as fruit aroma volatiles. Of the over 100 volatile compounds described in peach, lactones most likely have the stro...

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Main Authors: Sanchez, Gerardo, Venegas Calerón, Mónica, Salas, Joaquín Jesús, Monforte, Antonio José, Badenes, María Luisa, Granell, Antonio
Format: info:ar-repo/semantics/artículo
Language:Inglés
Published: 2017
Subjects:
Online Access:http://hdl.handle.net/20.500.12123/1466
https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-14-343
https://doi.org/10.1186/1471-2164-14-343
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author Sanchez, Gerardo
Venegas Calerón, Mónica
Salas, Joaquín Jesús
Monforte, Antonio José
Badenes, María Luisa
Granell, Antonio
author_browse Badenes, María Luisa
Granell, Antonio
Monforte, Antonio José
Salas, Joaquín Jesús
Sanchez, Gerardo
Venegas Calerón, Mónica
author_facet Sanchez, Gerardo
Venegas Calerón, Mónica
Salas, Joaquín Jesús
Monforte, Antonio José
Badenes, María Luisa
Granell, Antonio
author_sort Sanchez, Gerardo
collection INTA Digital
description Background: Ever since the recent completion of the peach genome, the focus of genetic research in this area has turned to the identification of genes related to important traits, such as fruit aroma volatiles. Of the over 100 volatile compounds described in peach, lactones most likely have the strongest effect on fruit aroma, while esters, terpenoids, and aldehydes have minor, yet significant effects. The identification of key genes underlying the production of aroma compounds is of interest for any fruit-quality improvement strategy. Results: Volatile (52 compounds) and gene expression (4348 genes) levels were profiled in peach fruit from a maturity time-course series belonging to two peach genotypes that showed considerable differences in maturation characteristics and postharvest ripening. This data set was analyzed by complementary correlation-based approaches to discover the genes related to the main aroma-contributing compounds: lactones, esters, and phenolic volatiles, among others. As a case study, one of the candidate genes was cloned and expressed in yeast to show specificity as an ω-6 Oleate desaturase, which may be involved in the production of a precursor of lactones/esters. Conclusions: Our approach revealed a set of genes (an alcohol acyl transferase, fatty acid desaturases, transcription factors, protein kinases, cytochromes, etc.) that are highly associated with peach fruit volatiles, and which could prove useful in breeding or for biotechnological purposes.
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institution Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina)
language Inglés
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spelling INTA14662019-02-22T14:23:48Z An integrative 'omics' approach identifies new candidate genes to impact aroma volatiles in peach fruit Sanchez, Gerardo Venegas Calerón, Mónica Salas, Joaquín Jesús Monforte, Antonio José Badenes, María Luisa Granell, Antonio Durazno Peaches Organic Volatile Compounds Flavour Aroma Precursors Genomes Prunus Persica Compuestos Orgánicos Volátiles Sabor Precursor de Aroma Genomas Ciencias Omicas Background: Ever since the recent completion of the peach genome, the focus of genetic research in this area has turned to the identification of genes related to important traits, such as fruit aroma volatiles. Of the over 100 volatile compounds described in peach, lactones most likely have the strongest effect on fruit aroma, while esters, terpenoids, and aldehydes have minor, yet significant effects. The identification of key genes underlying the production of aroma compounds is of interest for any fruit-quality improvement strategy. Results: Volatile (52 compounds) and gene expression (4348 genes) levels were profiled in peach fruit from a maturity time-course series belonging to two peach genotypes that showed considerable differences in maturation characteristics and postharvest ripening. This data set was analyzed by complementary correlation-based approaches to discover the genes related to the main aroma-contributing compounds: lactones, esters, and phenolic volatiles, among others. As a case study, one of the candidate genes was cloned and expressed in yeast to show specificity as an ω-6 Oleate desaturase, which may be involved in the production of a precursor of lactones/esters. Conclusions: Our approach revealed a set of genes (an alcohol acyl transferase, fatty acid desaturases, transcription factors, protein kinases, cytochromes, etc.) that are highly associated with peach fruit volatiles, and which could prove useful in breeding or for biotechnological purposes. Fil: Sanchez, Gerardo. Consejo Superior de Investigaciones Científicas. Instituto de Biología Molecular y Celular de Plantas; España. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina Fil: Venegas Calerón, Mónica. Consejo Superior de Investigaciones Científicas. Instituto de la Grasa; España Fil: Salas, Joaquín Jesús. Consejo Superior de Investigaciones Científicas. Instituto de la Grasa; España Fil: Monforte, Antonio José. Consejo Superior de Investigaciones Científicas. Instituto de Biología Molecular y Celular de Plantas; España Fil: Badenes, Maria Luisa. Instituto Valenciano de Investigaciones Agrarias (IVIA); España Fil: Granell, Antonio. Consejo Superior de Investigaciones Científicas. Instituto de Biología Molecular y Celular de Plantas; España 2017-10-11T18:54:11Z 2017-10-11T18:54:11Z 2013-05-23 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/1466 https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-14-343 1471-2164 https://doi.org/10.1186/1471-2164-14-343 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf BMC genomics 14 : 1-23. (2013)
spellingShingle Durazno
Peaches
Organic Volatile Compounds
Flavour
Aroma Precursors
Genomes
Prunus Persica
Compuestos Orgánicos Volátiles
Sabor
Precursor de Aroma
Genomas
Ciencias Omicas
Sanchez, Gerardo
Venegas Calerón, Mónica
Salas, Joaquín Jesús
Monforte, Antonio José
Badenes, María Luisa
Granell, Antonio
An integrative 'omics' approach identifies new candidate genes to impact aroma volatiles in peach fruit
title An integrative 'omics' approach identifies new candidate genes to impact aroma volatiles in peach fruit
title_full An integrative 'omics' approach identifies new candidate genes to impact aroma volatiles in peach fruit
title_fullStr An integrative 'omics' approach identifies new candidate genes to impact aroma volatiles in peach fruit
title_full_unstemmed An integrative 'omics' approach identifies new candidate genes to impact aroma volatiles in peach fruit
title_short An integrative 'omics' approach identifies new candidate genes to impact aroma volatiles in peach fruit
title_sort integrative omics approach identifies new candidate genes to impact aroma volatiles in peach fruit
topic Durazno
Peaches
Organic Volatile Compounds
Flavour
Aroma Precursors
Genomes
Prunus Persica
Compuestos Orgánicos Volátiles
Sabor
Precursor de Aroma
Genomas
Ciencias Omicas
url http://hdl.handle.net/20.500.12123/1466
https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-14-343
https://doi.org/10.1186/1471-2164-14-343
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