Differential replication of Foot-and-mouth disease viruses in mice determine lethality
Adult C57BL/6J mice have been used to study Foot-and-mouth disease virus (FMDV) biology. In this work, two variants of an FMDV A/Arg/01 strain exhibiting differential pathogenicity in adult mice were identified and characterized: a non-lethal virus (A01NL) caused mild signs of disease, whereas a let...
| Autores principales: | , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/1464 http://www.sciencedirect.com/science/article/pii/S0042682217301903 https://doi.org/10.1016/j.virol.2017.06.012 |
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| author | Cacciabue, Marco Polo Garcia Nuñez, Maria Soledad Delgado, Fernando Oscar Curra, Anabella Paola Marrero Diaz De Villegas, Rubén Molinari, Maria Paula Rieder, Elizabeth Carrillo, Elisa Cristina Gismondi, Maria Ines |
| author_browse | Cacciabue, Marco Polo Carrillo, Elisa Cristina Curra, Anabella Paola Delgado, Fernando Oscar Garcia Nuñez, Maria Soledad Gismondi, Maria Ines Marrero Diaz De Villegas, Rubén Molinari, Maria Paula Rieder, Elizabeth |
| author_facet | Cacciabue, Marco Polo Garcia Nuñez, Maria Soledad Delgado, Fernando Oscar Curra, Anabella Paola Marrero Diaz De Villegas, Rubén Molinari, Maria Paula Rieder, Elizabeth Carrillo, Elisa Cristina Gismondi, Maria Ines |
| author_sort | Cacciabue, Marco Polo |
| collection | INTA Digital |
| description | Adult C57BL/6J mice have been used to study Foot-and-mouth disease virus (FMDV) biology. In this work, two variants of an FMDV A/Arg/01 strain exhibiting differential pathogenicity in adult mice were identified and characterized: a non-lethal virus (A01NL) caused mild signs of disease, whereas a lethal virus (A01L) caused death within 24–48 h independently of the dose used. Both viruses caused a systemic infection with pathological changes in the exocrine pancreas. Virus A01L reached higher viral loads in plasma and organs of inoculated mice as well as increased replication in an ovine kidney cell line. Complete consensus sequences revealed 6 nonsynonymous changes between A01L and A10NL genomes that might be linked to replication differences, as suggested by in silico prediction studies. Our results highlight the biological significance of discrete genomic variations and reinforce the usefulness of this animal model to study viral determinants of lethality. |
| format | Artículo |
| id | INTA1464 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA14642019-03-22T15:08:23Z Differential replication of Foot-and-mouth disease viruses in mice determine lethality Cacciabue, Marco Polo Garcia Nuñez, Maria Soledad Delgado, Fernando Oscar Curra, Anabella Paola Marrero Diaz De Villegas, Rubén Molinari, Maria Paula Rieder, Elizabeth Carrillo, Elisa Cristina Gismondi, Maria Ines Ratón Fiebre Aftosa Patogénesis Mice Foot and Mouth Disease Pathogenesis Adult C57BL/6J mice have been used to study Foot-and-mouth disease virus (FMDV) biology. In this work, two variants of an FMDV A/Arg/01 strain exhibiting differential pathogenicity in adult mice were identified and characterized: a non-lethal virus (A01NL) caused mild signs of disease, whereas a lethal virus (A01L) caused death within 24–48 h independently of the dose used. Both viruses caused a systemic infection with pathological changes in the exocrine pancreas. Virus A01L reached higher viral loads in plasma and organs of inoculated mice as well as increased replication in an ovine kidney cell line. Complete consensus sequences revealed 6 nonsynonymous changes between A01L and A10NL genomes that might be linked to replication differences, as suggested by in silico prediction studies. Our results highlight the biological significance of discrete genomic variations and reinforce the usefulness of this animal model to study viral determinants of lethality. Inst. de Biotecnología Fil: Cacciabue, Marco Polo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Garcia Nuñez, Maria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Delgado, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Curra, Anabella Paola. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Marrero Diaz De Villegas, Rubén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Molinari, Maria Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Carrillo, Elisa Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gismondi, Maria Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2017-10-11T15:27:35Z 2017-10-11T15:27:35Z 2017-09 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/1464 http://www.sciencedirect.com/science/article/pii/S0042682217301903 0042-6822 https://doi.org/10.1016/j.virol.2017.06.012 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Virology 509 : 195-204. (September 2017) |
| spellingShingle | Ratón Fiebre Aftosa Patogénesis Mice Foot and Mouth Disease Pathogenesis Cacciabue, Marco Polo Garcia Nuñez, Maria Soledad Delgado, Fernando Oscar Curra, Anabella Paola Marrero Diaz De Villegas, Rubén Molinari, Maria Paula Rieder, Elizabeth Carrillo, Elisa Cristina Gismondi, Maria Ines Differential replication of Foot-and-mouth disease viruses in mice determine lethality |
| title | Differential replication of Foot-and-mouth disease viruses in mice determine lethality |
| title_full | Differential replication of Foot-and-mouth disease viruses in mice determine lethality |
| title_fullStr | Differential replication of Foot-and-mouth disease viruses in mice determine lethality |
| title_full_unstemmed | Differential replication of Foot-and-mouth disease viruses in mice determine lethality |
| title_short | Differential replication of Foot-and-mouth disease viruses in mice determine lethality |
| title_sort | differential replication of foot and mouth disease viruses in mice determine lethality |
| topic | Ratón Fiebre Aftosa Patogénesis Mice Foot and Mouth Disease Pathogenesis |
| url | http://hdl.handle.net/20.500.12123/1464 http://www.sciencedirect.com/science/article/pii/S0042682217301903 https://doi.org/10.1016/j.virol.2017.06.012 |
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