Convergent Adaptation of Multiple Herbicide Resistance to Auxin Mimics and ALS- and EPSPS-Inhibitors in Brassica rapa from North and South America
Herbicide-resistant weeds have been identified and recorded on every continent where croplands are available. Despite the diversity of weed communities, it is of interest how selection has led to the same consequences in distant regions. Brassica rapa is a widespread naturalized weed that is found t...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Artículo |
| Language: | Inglés |
| Published: |
MDPI
2023
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/14636 https://www.mdpi.com/2223-7747/12/11/2119 https://doi.org/10.3390/plants12112119 |
| _version_ | 1855485399697195008 |
|---|---|
| author | Dominguez-Valenzuela, José Alfredo Palma Bautista, Candelario Vázquez García, José Guadalupe Yanniccari, Marcos Gigon, Ramon Alcántara de la Cruz, Ricardo de Prado, Rafael Portugal, João |
| author_browse | Alcántara de la Cruz, Ricardo Dominguez-Valenzuela, José Alfredo Gigon, Ramon Palma Bautista, Candelario Portugal, João Vázquez García, José Guadalupe Yanniccari, Marcos de Prado, Rafael |
| author_facet | Dominguez-Valenzuela, José Alfredo Palma Bautista, Candelario Vázquez García, José Guadalupe Yanniccari, Marcos Gigon, Ramon Alcántara de la Cruz, Ricardo de Prado, Rafael Portugal, João |
| author_sort | Dominguez-Valenzuela, José Alfredo |
| collection | INTA Digital |
| description | Herbicide-resistant weeds have been identified and recorded on every continent where croplands are available. Despite the diversity of weed communities, it is of interest how selection has led to the same consequences in distant regions. Brassica rapa is a widespread naturalized weed that is found throughout temperate North and South America, and it is a frequent weed among winter cereal crops in Argentina and in Mexico. Broadleaf weed control is based on glyphosate that is used prior to sowing and sulfonylureas or mimic auxin herbicides that are used once the weeds have already emerged. This study was aimed at determining whether a convergent phenotypic adaptation to multiple herbicides had occurred in B. rapa populations from Mexico and Argentina by comparing the herbicide sensitivity to inhibitors of the acetolactate synthase (ALS), 5-enolpyruvylshikimate-3-phosphate (EPSPS), and auxin mimics. Five B. rapa populations were analyzed from seeds collected in wheat fields in Argentina (Ar1 and Ar2) and barley fields in Mexico (Mx1, Mx2 and MxS). Mx1, Mx2, and Ar1 populations presented multiple resistance to ALS- and EPSPS-inhibitors and to auxin mimics (2,4-D, MCPA, and fluroxypyr), while the Ar2 population showed resistance only to ALS-inhibitors and glyphosate. Resistance factors ranged from 947 to 4069 for tribenuron-methyl, from 1.5 to 9.4 for 2,4-D, and from 2.7 to 42 for glyphosate. These were consistent with ALS activity, ethylene production, and shikimate accumulation analyses in response to tribenuron-methyl, 2,4-D, and glyphosate, respectively. These results fully support the evolution of the multiple- and cross-herbicide resistance to glyphosate, ALS-inhibitors, and auxinic herbicides in B. rapa populations from Mexico and Argentina. |
| format | Artículo |
| id | INTA14636 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INTA146362023-06-29T11:13:27Z Convergent Adaptation of Multiple Herbicide Resistance to Auxin Mimics and ALS- and EPSPS-Inhibitors in Brassica rapa from North and South America Dominguez-Valenzuela, José Alfredo Palma Bautista, Candelario Vázquez García, José Guadalupe Yanniccari, Marcos Gigon, Ramon Alcántara de la Cruz, Ricardo de Prado, Rafael Portugal, João Auxinas Resistencia a los Herbicidas Brassica rapa Argentina Mexico Semilla de Colza Auxins Herbicide Resistance Rapeseed Acetolactate Synthase 5-enolpyruvylshikimate-3-phosphate Herbicide-resistant weeds have been identified and recorded on every continent where croplands are available. Despite the diversity of weed communities, it is of interest how selection has led to the same consequences in distant regions. Brassica rapa is a widespread naturalized weed that is found throughout temperate North and South America, and it is a frequent weed among winter cereal crops in Argentina and in Mexico. Broadleaf weed control is based on glyphosate that is used prior to sowing and sulfonylureas or mimic auxin herbicides that are used once the weeds have already emerged. This study was aimed at determining whether a convergent phenotypic adaptation to multiple herbicides had occurred in B. rapa populations from Mexico and Argentina by comparing the herbicide sensitivity to inhibitors of the acetolactate synthase (ALS), 5-enolpyruvylshikimate-3-phosphate (EPSPS), and auxin mimics. Five B. rapa populations were analyzed from seeds collected in wheat fields in Argentina (Ar1 and Ar2) and barley fields in Mexico (Mx1, Mx2 and MxS). Mx1, Mx2, and Ar1 populations presented multiple resistance to ALS- and EPSPS-inhibitors and to auxin mimics (2,4-D, MCPA, and fluroxypyr), while the Ar2 population showed resistance only to ALS-inhibitors and glyphosate. Resistance factors ranged from 947 to 4069 for tribenuron-methyl, from 1.5 to 9.4 for 2,4-D, and from 2.7 to 42 for glyphosate. These were consistent with ALS activity, ethylene production, and shikimate accumulation analyses in response to tribenuron-methyl, 2,4-D, and glyphosate, respectively. These results fully support the evolution of the multiple- and cross-herbicide resistance to glyphosate, ALS-inhibitors, and auxinic herbicides in B. rapa populations from Mexico and Argentina. EEA Barrow Fil: Dominguez-Valenzuela, José Alfredo. Universidad Autónoma de Chapingo. Department of Agricultural Parasitology; México Fil: Palma Bautista, Candelario. Universidad de Córdoba. Department of Agroforestry, Plant Biochemistry and Molecular Biology; España Fil: Vázquez García, José G. Universidad de Córdoba. Department of Agroforestry, Plant Biochemistry and Molecular Biology; España Fil: Yanniccari, Marcos Ezequiel. Instituto Nacional de Tecnología Agropecuaria (INTA). Chacra Experimental Integrada Barrow; Argentina Fil: Yanniccari, Marcos Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Yanniccari, Marcos Ezequiel. Universidad Nacional de La Pampa. Facultad de Agronomía; Argentina Fil: Gigón, Ramón. Consultor privado en Control de Malezas; Argentina Fil: Alcántara de la Cruz, Ricardo. Universidade Federal de São Carlos. Departamento de Química; Brasil Fil: de Prado, Rafael. Universidad de Córdoba. Department of Agroforestry, Plant Biochemistry and Molecular Biology; España Fil: Portugal, João. Polytechnic Institute of Beja. Biosciences Department; Portugal Fil: Portugal, João. Polytechnic Institute of Portalegre. VALORIZA-Research Centre for Endogenous Resource Valorization; Portugal 2023-06-16T16:05:41Z 2023-06-16T16:05:41Z 2023-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/14636 https://www.mdpi.com/2223-7747/12/11/2119 2223-7747 https://doi.org/10.3390/plants12112119 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Plants 12 (11) : 2119. (May 2023) |
| spellingShingle | Auxinas Resistencia a los Herbicidas Brassica rapa Argentina Mexico Semilla de Colza Auxins Herbicide Resistance Rapeseed Acetolactate Synthase 5-enolpyruvylshikimate-3-phosphate Dominguez-Valenzuela, José Alfredo Palma Bautista, Candelario Vázquez García, José Guadalupe Yanniccari, Marcos Gigon, Ramon Alcántara de la Cruz, Ricardo de Prado, Rafael Portugal, João Convergent Adaptation of Multiple Herbicide Resistance to Auxin Mimics and ALS- and EPSPS-Inhibitors in Brassica rapa from North and South America |
| title | Convergent Adaptation of Multiple Herbicide Resistance to Auxin Mimics and ALS- and EPSPS-Inhibitors in Brassica rapa from North and South America |
| title_full | Convergent Adaptation of Multiple Herbicide Resistance to Auxin Mimics and ALS- and EPSPS-Inhibitors in Brassica rapa from North and South America |
| title_fullStr | Convergent Adaptation of Multiple Herbicide Resistance to Auxin Mimics and ALS- and EPSPS-Inhibitors in Brassica rapa from North and South America |
| title_full_unstemmed | Convergent Adaptation of Multiple Herbicide Resistance to Auxin Mimics and ALS- and EPSPS-Inhibitors in Brassica rapa from North and South America |
| title_short | Convergent Adaptation of Multiple Herbicide Resistance to Auxin Mimics and ALS- and EPSPS-Inhibitors in Brassica rapa from North and South America |
| title_sort | convergent adaptation of multiple herbicide resistance to auxin mimics and als and epsps inhibitors in brassica rapa from north and south america |
| topic | Auxinas Resistencia a los Herbicidas Brassica rapa Argentina Mexico Semilla de Colza Auxins Herbicide Resistance Rapeseed Acetolactate Synthase 5-enolpyruvylshikimate-3-phosphate |
| url | http://hdl.handle.net/20.500.12123/14636 https://www.mdpi.com/2223-7747/12/11/2119 https://doi.org/10.3390/plants12112119 |
| work_keys_str_mv | AT dominguezvalenzuelajosealfredo convergentadaptationofmultipleherbicideresistancetoauxinmimicsandalsandepspsinhibitorsinbrassicarapafromnorthandsouthamerica AT palmabautistacandelario convergentadaptationofmultipleherbicideresistancetoauxinmimicsandalsandepspsinhibitorsinbrassicarapafromnorthandsouthamerica AT vazquezgarciajoseguadalupe convergentadaptationofmultipleherbicideresistancetoauxinmimicsandalsandepspsinhibitorsinbrassicarapafromnorthandsouthamerica AT yanniccarimarcos convergentadaptationofmultipleherbicideresistancetoauxinmimicsandalsandepspsinhibitorsinbrassicarapafromnorthandsouthamerica AT gigonramon convergentadaptationofmultipleherbicideresistancetoauxinmimicsandalsandepspsinhibitorsinbrassicarapafromnorthandsouthamerica AT alcantaradelacruzricardo convergentadaptationofmultipleherbicideresistancetoauxinmimicsandalsandepspsinhibitorsinbrassicarapafromnorthandsouthamerica AT depradorafael convergentadaptationofmultipleherbicideresistancetoauxinmimicsandalsandepspsinhibitorsinbrassicarapafromnorthandsouthamerica AT portugaljoao convergentadaptationofmultipleherbicideresistancetoauxinmimicsandalsandepspsinhibitorsinbrassicarapafromnorthandsouthamerica |