Higher diversity of Brucella canis in Latin America, according to an MLVA_13 Bc analysis
Brucella canis is the main causative agent of canine brucellosis, which affects domestic and wild canids and leads to clinical signs and symptoms of the reproductive and locomotor systems. Owing to the scarce information on this pathogen, here we addressed the genetic diversity of the circulating st...
| Autores principales: | , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2023
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/14625 https://www.sciencedirect.com/science/article/pii/S0001706X23001018 https://doi.org/10.1016/j.actatropica.2023.106914 |
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| author | Boeri, Eduardo Jorge Ruybal, Paula Dominguez, María Luz Fernandez, Natalia Mercedes Becker, Paula Elena, Sebastián Escobar, Gabriela Ileana Ayala, Sandra Marcela Hasan, Deborah Beatriz Trangoni, Marcos David |
| author_browse | Ayala, Sandra Marcela Becker, Paula Boeri, Eduardo Jorge Dominguez, María Luz Elena, Sebastián Escobar, Gabriela Ileana Fernandez, Natalia Mercedes Hasan, Deborah Beatriz Ruybal, Paula Trangoni, Marcos David |
| author_facet | Boeri, Eduardo Jorge Ruybal, Paula Dominguez, María Luz Fernandez, Natalia Mercedes Becker, Paula Elena, Sebastián Escobar, Gabriela Ileana Ayala, Sandra Marcela Hasan, Deborah Beatriz Trangoni, Marcos David |
| author_sort | Boeri, Eduardo Jorge |
| collection | INTA Digital |
| description | Brucella canis is the main causative agent of canine brucellosis, which affects domestic and wild canids and leads to clinical signs and symptoms of the reproductive and locomotor systems. Owing to the scarce information on this pathogen, here we addressed the genetic diversity of the circulating strains of this species in Argentina by following an MVLA_13 Bc scheme. The analyzed sample set consisted of 101 strains of B. canis isolates collected between 2006 and 2020 from canines of the Autonomous City of Buenos Aires (CABA) and other regions of Argentina, as well as 235 isolates from North America. The analysis yielded 336 variants (Hunter–Gaston Diversity Index, HGDI equal to 1.0) showing high diversity on a global scale. The analysis of the six most variable markers also reveled high diversity and allowed further analysis regarding variant relationships. Although the diversity obtained using both schemes (all or the 6 most variable markers) was higher for the Latin American than for the North American strains, we cannot discard that this was due to biases in the sampling methodology or to the different health policies employed in these regions regarding the management of infected individuals. Altogether, the Argentine circulating strains are genetically diverse, but with no apparent geographical association. The markers used in the MLVA_13 Bc are variable and highly useful for the evaluation of outbreaks. Furthermore, the reduced panel of 6 markers (MLVA_6 Bc) proposed in this study is convenient for the study of B. canis strain diversity. |
| format | Artículo |
| id | INTA14625 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA146252023-04-28T16:04:16Z Higher diversity of Brucella canis in Latin America, according to an MLVA_13 Bc analysis Boeri, Eduardo Jorge Ruybal, Paula Dominguez, María Luz Fernandez, Natalia Mercedes Becker, Paula Elena, Sebastián Escobar, Gabriela Ileana Ayala, Sandra Marcela Hasan, Deborah Beatriz Trangoni, Marcos David Brucella canis Diversidad Genética (como Recurso) Brucelosis Enfermedades de los Animales Genetic Diversity (as Resource) Brucellosis Animal Diseases Brucella canis is the main causative agent of canine brucellosis, which affects domestic and wild canids and leads to clinical signs and symptoms of the reproductive and locomotor systems. Owing to the scarce information on this pathogen, here we addressed the genetic diversity of the circulating strains of this species in Argentina by following an MVLA_13 Bc scheme. The analyzed sample set consisted of 101 strains of B. canis isolates collected between 2006 and 2020 from canines of the Autonomous City of Buenos Aires (CABA) and other regions of Argentina, as well as 235 isolates from North America. The analysis yielded 336 variants (Hunter–Gaston Diversity Index, HGDI equal to 1.0) showing high diversity on a global scale. The analysis of the six most variable markers also reveled high diversity and allowed further analysis regarding variant relationships. Although the diversity obtained using both schemes (all or the 6 most variable markers) was higher for the Latin American than for the North American strains, we cannot discard that this was due to biases in the sampling methodology or to the different health policies employed in these regions regarding the management of infected individuals. Altogether, the Argentine circulating strains are genetically diverse, but with no apparent geographical association. The markers used in the MLVA_13 Bc are variable and highly useful for the evaluation of outbreaks. Furthermore, the reduced panel of 6 markers (MLVA_6 Bc) proposed in this study is convenient for the study of B. canis strain diversity. Instituto de Biotecnología Fil: Boeri, Eduardo Jorge. Instituto de Zoonosis Luis Pasteur. División Inmunología y Diagnóstico. Laboratorio Sección Serología y Diagnóstico; Argentina Fil: Ruybal, Paula. Universidad de Buenos Aires. Facultad de Medicina. Departamento de de Microbiología; Argentina Fil: Ruybal, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Dominguez, María Luz. Instituto de Zoonosis Luis Pasteur. División Inmunología y Diagnóstico. Laboratorio Sección Serología y Diagnóstico; Argentina Fil: Fernandez, Natalia Mercedes. Instituto de Zoonosis Luis Pasteur. División Inmunología y Diagnóstico. Laboratorio Sección Serología y Diagnóstico; Argentina Fil: Becker, Paula. Instituto de Zoonosis Luis Pasteur. División Inmunología y Diagnóstico. Laboratorio Sección Serología y Diagnóstico; Argentina Fil: Elena, Sebastián. Servicio Nacional de Sanidad y Calidad Agroalimentaria. Dirección General de Laboratorio y Control Técnico. Laboratorio de Referencia de la OIE para Brucelosis; Argentina Fil: Escobar, Gabriela Ileana. Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos Malbrán (ANLIS). Instituto Nacional de Enfermedades Infecciosas; Argentina Fil: Ayala, Sandra Marcela. Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos Malbrán (ANLIS). Instituto Nacional de Enfermedades Infecciosas; Argentina Fil: Hasan, Deborah Beatriz. Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos Malbrán (ANLIS). Instituto Nacional de Enfermedades Infecciosas; Argentina Fil: Trangoni, Marcos David. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular. Argentina Fil: Trangoni, Marcos David. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2023-04-28T16:02:07Z 2023-04-28T16:02:07Z 2023-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/14625 https://www.sciencedirect.com/science/article/pii/S0001706X23001018 0001-706X 1873-6254 https://doi.org/10.1016/j.actatropica.2023.106914 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Latin America .......... (general region) (World) 4006155 Elsevier Acta Tropica 243 : 106914 (July 2023) |
| spellingShingle | Brucella canis Diversidad Genética (como Recurso) Brucelosis Enfermedades de los Animales Genetic Diversity (as Resource) Brucellosis Animal Diseases Boeri, Eduardo Jorge Ruybal, Paula Dominguez, María Luz Fernandez, Natalia Mercedes Becker, Paula Elena, Sebastián Escobar, Gabriela Ileana Ayala, Sandra Marcela Hasan, Deborah Beatriz Trangoni, Marcos David Higher diversity of Brucella canis in Latin America, according to an MLVA_13 Bc analysis |
| title | Higher diversity of Brucella canis in Latin America, according to an MLVA_13 Bc analysis |
| title_full | Higher diversity of Brucella canis in Latin America, according to an MLVA_13 Bc analysis |
| title_fullStr | Higher diversity of Brucella canis in Latin America, according to an MLVA_13 Bc analysis |
| title_full_unstemmed | Higher diversity of Brucella canis in Latin America, according to an MLVA_13 Bc analysis |
| title_short | Higher diversity of Brucella canis in Latin America, according to an MLVA_13 Bc analysis |
| title_sort | higher diversity of brucella canis in latin america according to an mlva 13 bc analysis |
| topic | Brucella canis Diversidad Genética (como Recurso) Brucelosis Enfermedades de los Animales Genetic Diversity (as Resource) Brucellosis Animal Diseases |
| url | http://hdl.handle.net/20.500.12123/14625 https://www.sciencedirect.com/science/article/pii/S0001706X23001018 https://doi.org/10.1016/j.actatropica.2023.106914 |
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