Intraspecific differentiation in genetic structure in Panicum coloratum L.: importance for germplasm conservation and breeding
Success in a breeding program is highly dependent on the information regarding genetic variability and its structure of the available germplasm. Particularly in poorly domesticated species, such information is generally scarce. Panicum coloratum L. is a warm-season perennial grass with potential to...
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Springer
2023
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/14588 https://link.springer.com/article/10.1007/s10722-022-01530-3 https://doi.org/10.1007/s10722-022-01530-3 |
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| author | Armando, Lorena Vanesa Almada, Pamela Estefania Tomas, Maria Andrea |
| author_browse | Almada, Pamela Estefania Armando, Lorena Vanesa Tomas, Maria Andrea |
| author_facet | Armando, Lorena Vanesa Almada, Pamela Estefania Tomas, Maria Andrea |
| author_sort | Armando, Lorena Vanesa |
| collection | INTA Digital |
| description | Success in a breeding program is highly dependent on the information regarding genetic variability and its structure of the available germplasm. Particularly in poorly domesticated species, such information is generally scarce. Panicum coloratum L. is a warm-season perennial grass with potential to increase production in agricultural degraded areas. Its two varieties have high genetic polymorphism and morphological overlapping. The goal of the present study was to assess the genetic diversity and intraspecific structure of a collection of P. coloratum using ISSR molecular markers. Eight accessions of var. makarikariense Gooss. and ten from var. coloratum, plus two commercial materials were analyzed. Differences in variability between varieties were evident: polymorphism and percent of variety specific loci were lower (79%P and 10.3%) in var. makarikariense than in var. coloratum (93%P and 30.9%). Analysis of molecular variation (AMOVA) revealed 13.7% (p = 0.001) of variation was attributable to differences between varieties, and 43.8% remained within the accessions. Individuals of var. coloratum showed higher mean genetic distance among them than the ones of var. makarikariense. The PCO biplot and the dendrogram showed some distinction between varieties, although some accessions of var. coloratum were close to var. makarikariense. Variation in var. coloratum agreed with an allogamous reproductive behavior. Alternatively, less variable closely grouped individuals in var. makarikariense, concur with frequent vegetative reproduction by rhizomes of a reduced number of genotypes. Our study showed that genetic variation had different structure between varieties. This difference should be taken into account when planning germplasm management and conservation and in designing breeding programs. |
| format | Artículo |
| id | INTA14588 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA145882023-04-26T18:31:37Z Intraspecific differentiation in genetic structure in Panicum coloratum L.: importance for germplasm conservation and breeding Armando, Lorena Vanesa Almada, Pamela Estefania Tomas, Maria Andrea Genetic Markers Intraspecific Relationships Germplasm Marcadores Genéticos Panicum coloratum Relaciones Intraespecíficas Germoplasma Genetic Diversity Molecular Markers Perennial Grass Diversidad Genética Marcadores Moleculares Pastura Perenne Success in a breeding program is highly dependent on the information regarding genetic variability and its structure of the available germplasm. Particularly in poorly domesticated species, such information is generally scarce. Panicum coloratum L. is a warm-season perennial grass with potential to increase production in agricultural degraded areas. Its two varieties have high genetic polymorphism and morphological overlapping. The goal of the present study was to assess the genetic diversity and intraspecific structure of a collection of P. coloratum using ISSR molecular markers. Eight accessions of var. makarikariense Gooss. and ten from var. coloratum, plus two commercial materials were analyzed. Differences in variability between varieties were evident: polymorphism and percent of variety specific loci were lower (79%P and 10.3%) in var. makarikariense than in var. coloratum (93%P and 30.9%). Analysis of molecular variation (AMOVA) revealed 13.7% (p = 0.001) of variation was attributable to differences between varieties, and 43.8% remained within the accessions. Individuals of var. coloratum showed higher mean genetic distance among them than the ones of var. makarikariense. The PCO biplot and the dendrogram showed some distinction between varieties, although some accessions of var. coloratum were close to var. makarikariense. Variation in var. coloratum agreed with an allogamous reproductive behavior. Alternatively, less variable closely grouped individuals in var. makarikariense, concur with frequent vegetative reproduction by rhizomes of a reduced number of genotypes. Our study showed that genetic variation had different structure between varieties. This difference should be taken into account when planning germplasm management and conservation and in designing breeding programs. EEA Rafaela Fil: Armando, Lorena Vanesa. Universidad Nacional del Sur. Departamento de Agronomía; Argentina Fil: Almada, Pamela Estefania. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina Fil: Tomas, Maria Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Tomas, Maria Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina 2023-04-26T18:18:01Z 2023-04-26T18:18:01Z 2023-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/14588 https://link.springer.com/article/10.1007/s10722-022-01530-3 Armando, L.V., Almada, P.E. & Tomás, M.A. Intraspecific differentiation in genetic structure in Panicum coloratum L.: importance for germplasm conservation and breeding. Genet Resour Crop Evol (2023). https://doi.org/10.1007/s10722-022-01530-3 1573-5109 (electronic) 0925-9864 (print) https://doi.org/10.1007/s10722-022-01530-3 eng info:eu-repograntAgreement/INTA/2019-PE-E6-I142-001/2019-PE-E6-I142-001/AR./Mejoramiento genético de leguminosas y gramíneas forrajeras para incrementar la productividad y la sustentabilidad de los sistemas agropecuarios de la Argentina info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Genetic Resources and Crop Evolution : 1-14. (Published: 02 February 2023) |
| spellingShingle | Genetic Markers Intraspecific Relationships Germplasm Marcadores Genéticos Panicum coloratum Relaciones Intraespecíficas Germoplasma Genetic Diversity Molecular Markers Perennial Grass Diversidad Genética Marcadores Moleculares Pastura Perenne Armando, Lorena Vanesa Almada, Pamela Estefania Tomas, Maria Andrea Intraspecific differentiation in genetic structure in Panicum coloratum L.: importance for germplasm conservation and breeding |
| title | Intraspecific differentiation in genetic structure in Panicum coloratum L.: importance for germplasm conservation and breeding |
| title_full | Intraspecific differentiation in genetic structure in Panicum coloratum L.: importance for germplasm conservation and breeding |
| title_fullStr | Intraspecific differentiation in genetic structure in Panicum coloratum L.: importance for germplasm conservation and breeding |
| title_full_unstemmed | Intraspecific differentiation in genetic structure in Panicum coloratum L.: importance for germplasm conservation and breeding |
| title_short | Intraspecific differentiation in genetic structure in Panicum coloratum L.: importance for germplasm conservation and breeding |
| title_sort | intraspecific differentiation in genetic structure in panicum coloratum l importance for germplasm conservation and breeding |
| topic | Genetic Markers Intraspecific Relationships Germplasm Marcadores Genéticos Panicum coloratum Relaciones Intraespecíficas Germoplasma Genetic Diversity Molecular Markers Perennial Grass Diversidad Genética Marcadores Moleculares Pastura Perenne |
| url | http://hdl.handle.net/20.500.12123/14588 https://link.springer.com/article/10.1007/s10722-022-01530-3 https://doi.org/10.1007/s10722-022-01530-3 |
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