Development and field evaluation of an ELISA to differentiate Anaplasma marginale–infected from A. centrale–vaccinated cattle
Immunization of calves with Anaplasma centrale is used to prevent acute anaplasmosis caused by A. marginale. Natural and vaccine-acquired immunity is detected through serologic tests based primarily on A. marginale recombinant major surface protein 5 (MSP5m) because it has 91% identity with MSP5 fro...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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American Association of Veterinary Laboratory Diagnosticians
2023
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| Online Access: | http://hdl.handle.net/20.500.12123/14583 https://journals.sagepub.com/doi/10.1177/10406387231152472 https://doi.org/10.1177/10406387231152472 |
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| author | Bellezze, Julio Thompson, Carolina Soledad Bosio, Anabela Torioni, Susana Marta Primo, María Evangelina |
| author_browse | Bellezze, Julio Bosio, Anabela Primo, María Evangelina Thompson, Carolina Soledad Torioni, Susana Marta |
| author_facet | Bellezze, Julio Thompson, Carolina Soledad Bosio, Anabela Torioni, Susana Marta Primo, María Evangelina |
| author_sort | Bellezze, Julio |
| collection | INTA Digital |
| description | Immunization of calves with Anaplasma centrale is used to prevent acute anaplasmosis caused by A. marginale. Natural and vaccine-acquired immunity is detected through serologic tests based primarily on A. marginale recombinant major surface protein 5 (MSP5m) because it has 91% identity with MSP5 from A. centrale (MSP5c). We developed a displacement, double-antigen, sandwich ELISA (ddasELISA) to detect antibodies against A. marginale or A. centrale. For ddasELISA validation, we analyzed serum samples positive for antibodies against Anaplasma spp. from cattle naturally infected with A. marginale (n = 300) or vaccinated with A. centrale (n = 255). Species-specific nested PCR (nPCR) assays were used to confirm infection. The optical density (OD) values obtained from antibodies directed at unique epitopes of A. marginale (ODAm) or A. centrale (ODAc) were used in the formula ODAm/ODAc. If the derived ratio was >0.38, the serum sample was considered positive for antibodies against A. marginale, with 98.9% sensitivity and 98.0% specificity. In a field evaluation, we analyzed 702 Anaplasma spp. antibody–positive serum samples from 34 herds by ddasELISA and nPCR; 571 were classified by ddasELISA as A. marginale–infected or A. centrale–vaccinated, with 84% agreement (κ = 0.70) between ddasELISA and nPCR. Our results indicate that ddasELISA could be used as a cost-effective alternative to molecular techniques to confirm infection with A. marginale in countries in which prevention is based on vaccination with A. centrale. |
| format | info:ar-repo/semantics/artículo |
| id | INTA14583 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | American Association of Veterinary Laboratory Diagnosticians |
| publisherStr | American Association of Veterinary Laboratory Diagnosticians |
| record_format | dspace |
| spelling | INTA145832023-04-26T17:05:10Z Development and field evaluation of an ELISA to differentiate Anaplasma marginale–infected from A. centrale–vaccinated cattle Bellezze, Julio Thompson, Carolina Soledad Bosio, Anabela Torioni, Susana Marta Primo, María Evangelina Anaplasma marginale Anaplasma centrale ELISA Infectious Diseases Cattle Enfermedades Infecciosas Anaplasmosis Ganado Bovino Species-specific Antibodies Anticuerpos Específicos de Especies Immunization of calves with Anaplasma centrale is used to prevent acute anaplasmosis caused by A. marginale. Natural and vaccine-acquired immunity is detected through serologic tests based primarily on A. marginale recombinant major surface protein 5 (MSP5m) because it has 91% identity with MSP5 from A. centrale (MSP5c). We developed a displacement, double-antigen, sandwich ELISA (ddasELISA) to detect antibodies against A. marginale or A. centrale. For ddasELISA validation, we analyzed serum samples positive for antibodies against Anaplasma spp. from cattle naturally infected with A. marginale (n = 300) or vaccinated with A. centrale (n = 255). Species-specific nested PCR (nPCR) assays were used to confirm infection. The optical density (OD) values obtained from antibodies directed at unique epitopes of A. marginale (ODAm) or A. centrale (ODAc) were used in the formula ODAm/ODAc. If the derived ratio was >0.38, the serum sample was considered positive for antibodies against A. marginale, with 98.9% sensitivity and 98.0% specificity. In a field evaluation, we analyzed 702 Anaplasma spp. antibody–positive serum samples from 34 herds by ddasELISA and nPCR; 571 were classified by ddasELISA as A. marginale–infected or A. centrale–vaccinated, with 84% agreement (κ = 0.70) between ddasELISA and nPCR. Our results indicate that ddasELISA could be used as a cost-effective alternative to molecular techniques to confirm infection with A. marginale in countries in which prevention is based on vaccination with A. centrale. INTA. Asociación Cooperadora INTA Rafaela (TCP 426100), ASATEC (IO-2019-130). Agencia Nacional de Promoción Científica y Tecnológica (PICT2013-0369, PICT2019-01498). EEA Rafaela Fil: Bellezze, Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Bellezze, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Thompson, Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Thompson, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Torioni, Susana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Torioni, Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Bosio, Anabela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Bosio, Anabela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Primo, María Evangelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Primo, María Evangelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina 2023-04-26T16:53:08Z 2023-04-26T16:53:08Z 2023-03 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/14583 https://journals.sagepub.com/doi/10.1177/10406387231152472 Bellezze J, Thompson CS, Bosio AS, Torioni SM, Primo ME. Development and field evaluation of an ELISA to differentiate Anaplasma marginale–infected from A. centrale–vaccinated cattle. Journal of Veterinary Diagnostic Investigation. 2023;35(2):204-208. doi:10.1177/10406387231152472 1943-4936 (electronic) 1040-6387 https://doi.org/10.1177/10406387231152472 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf American Association of Veterinary Laboratory Diagnosticians Journal of Veterinary Diagnostic Investigation 35 (2) : 204-208 (March 2023) |
| spellingShingle | Anaplasma marginale Anaplasma centrale ELISA Infectious Diseases Cattle Enfermedades Infecciosas Anaplasmosis Ganado Bovino Species-specific Antibodies Anticuerpos Específicos de Especies Bellezze, Julio Thompson, Carolina Soledad Bosio, Anabela Torioni, Susana Marta Primo, María Evangelina Development and field evaluation of an ELISA to differentiate Anaplasma marginale–infected from A. centrale–vaccinated cattle |
| title | Development and field evaluation of an ELISA to differentiate Anaplasma marginale–infected from A. centrale–vaccinated cattle |
| title_full | Development and field evaluation of an ELISA to differentiate Anaplasma marginale–infected from A. centrale–vaccinated cattle |
| title_fullStr | Development and field evaluation of an ELISA to differentiate Anaplasma marginale–infected from A. centrale–vaccinated cattle |
| title_full_unstemmed | Development and field evaluation of an ELISA to differentiate Anaplasma marginale–infected from A. centrale–vaccinated cattle |
| title_short | Development and field evaluation of an ELISA to differentiate Anaplasma marginale–infected from A. centrale–vaccinated cattle |
| title_sort | development and field evaluation of an elisa to differentiate anaplasma marginale infected from a centrale vaccinated cattle |
| topic | Anaplasma marginale Anaplasma centrale ELISA Infectious Diseases Cattle Enfermedades Infecciosas Anaplasmosis Ganado Bovino Species-specific Antibodies Anticuerpos Específicos de Especies |
| url | http://hdl.handle.net/20.500.12123/14583 https://journals.sagepub.com/doi/10.1177/10406387231152472 https://doi.org/10.1177/10406387231152472 |
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