MicroRNA analysis of Taenia crassiceps cysticerci under praziquantel treatment and genome-wide identification of Taenia solium miRNAs
MicroRNAs (miRNAs) are small non-coding RNAs that have emerged as important regulators of gene expression and perform critical functions in development and disease. In spite of the increased interest in miRNAs from helminth parasites, no information is available on miRNAs from Taenia solium, the cau...
| Autores principales: | , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/1458 http://www.sciencedirect.com/science/article/pii/S0020751917301315?via%3Dihub https://doi.org/10.1016/j.ijpara.2017.04.002 |
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| author | Pérez, Matías Macchiaroli, Natalia Lichtenstein, Gabriel Conti, Gabriela Asurmendi, Sebastian Milone, Diego Humberto Stegmayer, Georgina Kamenetzky, Laura Cucher, Marcela Rosenzvit, Mara Cecilia |
| author_browse | Asurmendi, Sebastian Conti, Gabriela Cucher, Marcela Kamenetzky, Laura Lichtenstein, Gabriel Macchiaroli, Natalia Milone, Diego Humberto Pérez, Matías Rosenzvit, Mara Cecilia Stegmayer, Georgina |
| author_facet | Pérez, Matías Macchiaroli, Natalia Lichtenstein, Gabriel Conti, Gabriela Asurmendi, Sebastian Milone, Diego Humberto Stegmayer, Georgina Kamenetzky, Laura Cucher, Marcela Rosenzvit, Mara Cecilia |
| author_sort | Pérez, Matías |
| collection | INTA Digital |
| description | MicroRNAs (miRNAs) are small non-coding RNAs that have emerged as important regulators of gene expression and perform critical functions in development and disease. In spite of the increased interest in miRNAs from helminth parasites, no information is available on miRNAs from Taenia solium, the causative agent of cysticercosis, a neglected disease affecting millions of people worldwide. Here we performed a comprehensive analysis of miRNAs from Taenia crassiceps, a laboratory model for T. solium studies, and identified miRNAs in the T. solium genome. Moreover, we analysed the effect of praziquantel, one of the two main drugs used for cysticercosis treatment, on the miRNA expression profile of T. crassiceps cysticerci. Using small RNA-seq and two independent algorithms for miRNA prediction, as well as northern blot validation, we found transcriptional evidence of 39 miRNA loci in T. crassiceps. Since miRNAs were mapped to the T. solium genome, these miRNAs are considered common to both parasites.
The miRNA expression profile of T. crassiceps was biased to the same set of highly expressed miRNAs reported in other cestodes. We found a significant altered expression of miR-7b under praziquantel treatment.
In addition, we searched for miRNAs predicted to target genes related to drug response. We performed a detailed target prediction for miR-7b and found genes related to drug action. We report an initial approach to study the effect of sub-lethal drug treatment on miRNA expression in a cestode parasite, which provides a platform for further studies of miRNA involvement in drug effects. The results of our work could be applied to drug development and provide basic knowledge of cysticercosis and other neglected helminth infections. |
| format | Artículo |
| id | INTA1458 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA14582018-06-19T17:21:58Z MicroRNA analysis of Taenia crassiceps cysticerci under praziquantel treatment and genome-wide identification of Taenia solium miRNAs Pérez, Matías Macchiaroli, Natalia Lichtenstein, Gabriel Conti, Gabriela Asurmendi, Sebastian Milone, Diego Humberto Stegmayer, Georgina Kamenetzky, Laura Cucher, Marcela Rosenzvit, Mara Cecilia Genética ARN Taenia Crassiceps Taenia Solium Identificación Genetics RNA Identification MicroRNAs (miRNAs) are small non-coding RNAs that have emerged as important regulators of gene expression and perform critical functions in development and disease. In spite of the increased interest in miRNAs from helminth parasites, no information is available on miRNAs from Taenia solium, the causative agent of cysticercosis, a neglected disease affecting millions of people worldwide. Here we performed a comprehensive analysis of miRNAs from Taenia crassiceps, a laboratory model for T. solium studies, and identified miRNAs in the T. solium genome. Moreover, we analysed the effect of praziquantel, one of the two main drugs used for cysticercosis treatment, on the miRNA expression profile of T. crassiceps cysticerci. Using small RNA-seq and two independent algorithms for miRNA prediction, as well as northern blot validation, we found transcriptional evidence of 39 miRNA loci in T. crassiceps. Since miRNAs were mapped to the T. solium genome, these miRNAs are considered common to both parasites. The miRNA expression profile of T. crassiceps was biased to the same set of highly expressed miRNAs reported in other cestodes. We found a significant altered expression of miR-7b under praziquantel treatment. In addition, we searched for miRNAs predicted to target genes related to drug response. We performed a detailed target prediction for miR-7b and found genes related to drug action. We report an initial approach to study the effect of sub-lethal drug treatment on miRNA expression in a cestode parasite, which provides a platform for further studies of miRNA involvement in drug effects. The results of our work could be applied to drug development and provide basic knowledge of cysticercosis and other neglected helminth infections. Inst. de Biotecnología Fil: Pérez, Matías. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Universidad de Buenos Aires. Facultad de Medicina;Argentina Fil: Macchiaroli, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Universidad de Buenos Aires. Facultad de Medicina;Argentina Fil: Lichtenstein, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Universidad de Buenos Aires. Facultad de Medicina;Argentina Fil: Conti, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Milone, Diego Humberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Sistemas e Inteligencia Computacion. Instituto de Investigación en Señales; Argentina Fil: Stegmayer, Georgina. Consejo Nacional de Investigaciones Científicas y Técnicas. Sistemas e Inteligencia Computacion. Instituto de Investigación en Señales; Argentina Fil: Kamenetzky, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina Fil: Cucher, Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Universidad de Buenos Aires. Facultad de Medicina;Argentina Rosenzvit, Mara Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina 2017-10-11T13:35:35Z 2017-10-11T13:35:35Z 2017-09 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/1458 http://www.sciencedirect.com/science/article/pii/S0020751917301315?via%3Dihub 0020-7519 (Print) 1879-0135 (Online) https://doi.org/10.1016/j.ijpara.2017.04.002 eng info:eu-repo/semantics/restrictedAccess application/pdf International journal for parasitology 47 (10–11) : 643-653 (September 2017) |
| spellingShingle | Genética ARN Taenia Crassiceps Taenia Solium Identificación Genetics RNA Identification Pérez, Matías Macchiaroli, Natalia Lichtenstein, Gabriel Conti, Gabriela Asurmendi, Sebastian Milone, Diego Humberto Stegmayer, Georgina Kamenetzky, Laura Cucher, Marcela Rosenzvit, Mara Cecilia MicroRNA analysis of Taenia crassiceps cysticerci under praziquantel treatment and genome-wide identification of Taenia solium miRNAs |
| title | MicroRNA analysis of Taenia crassiceps cysticerci under praziquantel treatment and genome-wide identification of Taenia solium miRNAs |
| title_full | MicroRNA analysis of Taenia crassiceps cysticerci under praziquantel treatment and genome-wide identification of Taenia solium miRNAs |
| title_fullStr | MicroRNA analysis of Taenia crassiceps cysticerci under praziquantel treatment and genome-wide identification of Taenia solium miRNAs |
| title_full_unstemmed | MicroRNA analysis of Taenia crassiceps cysticerci under praziquantel treatment and genome-wide identification of Taenia solium miRNAs |
| title_short | MicroRNA analysis of Taenia crassiceps cysticerci under praziquantel treatment and genome-wide identification of Taenia solium miRNAs |
| title_sort | microrna analysis of taenia crassiceps cysticerci under praziquantel treatment and genome wide identification of taenia solium mirnas |
| topic | Genética ARN Taenia Crassiceps Taenia Solium Identificación Genetics RNA Identification |
| url | http://hdl.handle.net/20.500.12123/1458 http://www.sciencedirect.com/science/article/pii/S0020751917301315?via%3Dihub https://doi.org/10.1016/j.ijpara.2017.04.002 |
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