Cestode parasites release extracellular vesicles with microRNAs and immunodiagnostic protein cargo
Intercellular communication is crucial in multiple aspects of cell biology. This interaction can be mediated by several mechanisms including extracellular vesicle (EV) transfer. EV secretion by parasites has been reported in protozoans, trematodes and nematodes. Here we report that this mechanism is...
| Autores principales: | , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/1457 http://www.sciencedirect.com/science/article/pii/S0020751917301984?via%3Dihub https://doi.org/10.1016/j.ijpara.2017.05.003 |
| _version_ | 1855482891512840192 |
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| author | Ancarola, María Eugenia Marcilla, Antonio Herz, Michaela Macchiaroli, Natalia Pérez, Matías Asurmendi, Sebastian Brehm, Klaus Poncini, Carolina Rosenzvit, Mara Cecilia Cucher, Marcela |
| author_browse | Ancarola, María Eugenia Asurmendi, Sebastian Brehm, Klaus Cucher, Marcela Herz, Michaela Macchiaroli, Natalia Marcilla, Antonio Poncini, Carolina Pérez, Matías Rosenzvit, Mara Cecilia |
| author_facet | Ancarola, María Eugenia Marcilla, Antonio Herz, Michaela Macchiaroli, Natalia Pérez, Matías Asurmendi, Sebastian Brehm, Klaus Poncini, Carolina Rosenzvit, Mara Cecilia Cucher, Marcela |
| author_sort | Ancarola, María Eugenia |
| collection | INTA Digital |
| description | Intercellular communication is crucial in multiple aspects of cell biology. This interaction can be mediated by several mechanisms including extracellular vesicle (EV) transfer. EV secretion by parasites has been reported in protozoans, trematodes and nematodes. Here we report that this mechanism is present in three different species of cestodes, Taenia crassiceps, Mesocestoides corti and Echinococcus multilocularis.
To confirm this we determined, in vitro, the presence of EVs in culture supernatants by transmission electron microscopy. Interestingly, while T. crassiceps and M. corti metacestodes secrete membranous structures into the culture media, similar vesicles were observed in the interface of the germinal and laminated layers of E. multilocularis metacestodes and were hardly detected in culture supernatants. We then determined the protein cargo in the EV-enriched secreted fractions of T. crassiceps and M. corti conditioned media by LC-MS/MS. Among the identified proteins, eukaryotic vesicle-enriched proteins were identified as expected, but also proteins used for cestode disease diagnosis, proteins related to neurotransmission, lipid binding proteins as well as host immunoglobulins and complement factors. Finally, we confirmed by capillary electrophoresis the presence of intravesicular RNA for both parasites and detected microRNAs by reverse transcription-PCR. This is the first report of EV secretion in cestode parasites and of an RNA secretion mechanism. These findings will provide valuable data not only for basic cestode biology but also for the rational search for new diagnostic targets. |
| format | Artículo |
| id | INTA1457 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA14572019-03-22T15:07:17Z Cestode parasites release extracellular vesicles with microRNAs and immunodiagnostic protein cargo Ancarola, María Eugenia Marcilla, Antonio Herz, Michaela Macchiaroli, Natalia Pérez, Matías Asurmendi, Sebastian Brehm, Klaus Poncini, Carolina Rosenzvit, Mara Cecilia Cucher, Marcela ARN Cestoda Platyhelminthes Mesocestoides Corti Echinococcus RNA Gusanos Planos Intercellular communication is crucial in multiple aspects of cell biology. This interaction can be mediated by several mechanisms including extracellular vesicle (EV) transfer. EV secretion by parasites has been reported in protozoans, trematodes and nematodes. Here we report that this mechanism is present in three different species of cestodes, Taenia crassiceps, Mesocestoides corti and Echinococcus multilocularis. To confirm this we determined, in vitro, the presence of EVs in culture supernatants by transmission electron microscopy. Interestingly, while T. crassiceps and M. corti metacestodes secrete membranous structures into the culture media, similar vesicles were observed in the interface of the germinal and laminated layers of E. multilocularis metacestodes and were hardly detected in culture supernatants. We then determined the protein cargo in the EV-enriched secreted fractions of T. crassiceps and M. corti conditioned media by LC-MS/MS. Among the identified proteins, eukaryotic vesicle-enriched proteins were identified as expected, but also proteins used for cestode disease diagnosis, proteins related to neurotransmission, lipid binding proteins as well as host immunoglobulins and complement factors. Finally, we confirmed by capillary electrophoresis the presence of intravesicular RNA for both parasites and detected microRNAs by reverse transcription-PCR. This is the first report of EV secretion in cestode parasites and of an RNA secretion mechanism. These findings will provide valuable data not only for basic cestode biology but also for the rational search for new diagnostic targets. Inst. de Biotecnología Fil: Ancarola, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina Fil: Herz, Michaela. University of Würzburg, Institute of Hygiene and Microbiology; Alemania Fil: Macchiaroli, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina Fil: Pérez, Matías. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Brehm, Klaus. University of Würzburg. Institute of Hygiene and Microbiology; Alemania Fil: Poncini, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina Fil: Rosenzvit, Mara Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina Fil: Cucher, Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina 2017-10-11T13:12:49Z 2017-10-11T13:12:49Z 2017-09 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/1457 http://www.sciencedirect.com/science/article/pii/S0020751917301984?via%3Dihub 0020-7519 (Print) 1879-0135 (Online) https://doi.org/10.1016/j.ijpara.2017.05.003 eng info:eu-repo/semantics/restrictedAccess application/pdf International journal for parasitology 47 (10–11) : 675-686. (September 2017) |
| spellingShingle | ARN Cestoda Platyhelminthes Mesocestoides Corti Echinococcus RNA Gusanos Planos Ancarola, María Eugenia Marcilla, Antonio Herz, Michaela Macchiaroli, Natalia Pérez, Matías Asurmendi, Sebastian Brehm, Klaus Poncini, Carolina Rosenzvit, Mara Cecilia Cucher, Marcela Cestode parasites release extracellular vesicles with microRNAs and immunodiagnostic protein cargo |
| title | Cestode parasites release extracellular vesicles with microRNAs and immunodiagnostic protein cargo |
| title_full | Cestode parasites release extracellular vesicles with microRNAs and immunodiagnostic protein cargo |
| title_fullStr | Cestode parasites release extracellular vesicles with microRNAs and immunodiagnostic protein cargo |
| title_full_unstemmed | Cestode parasites release extracellular vesicles with microRNAs and immunodiagnostic protein cargo |
| title_short | Cestode parasites release extracellular vesicles with microRNAs and immunodiagnostic protein cargo |
| title_sort | cestode parasites release extracellular vesicles with micrornas and immunodiagnostic protein cargo |
| topic | ARN Cestoda Platyhelminthes Mesocestoides Corti Echinococcus RNA Gusanos Planos |
| url | http://hdl.handle.net/20.500.12123/1457 http://www.sciencedirect.com/science/article/pii/S0020751917301984?via%3Dihub https://doi.org/10.1016/j.ijpara.2017.05.003 |
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