Modification of a previously patented method to unequivocally score A2-like and A1-like bovine β-casein variants
A growing interest in the production and commercialization of A2 cow's milk has been observed in many countries in the last few years due to the beneficial properties for human health attributed to A2 β-casein variant. Methods of varying complexity and different equipment requirements have been prop...
| Main Authors: | , , , |
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Elsevier
2023
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/14567 https://www.sciencedirect.com/science/article/pii/S2215016123001838 https://doi.org/10.1016/j.mex.2023.102183 |
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| author | Raschia, Maria Agustina Caffaro, María Eugenia Rossi, Ursula Amaranta Poli, Mario Andres |
| author_browse | Caffaro, María Eugenia Poli, Mario Andres Raschia, Maria Agustina Rossi, Ursula Amaranta |
| author_facet | Raschia, Maria Agustina Caffaro, María Eugenia Rossi, Ursula Amaranta Poli, Mario Andres |
| author_sort | Raschia, Maria Agustina |
| collection | INTA Digital |
| description | A growing interest in the production and commercialization of A2 cow's milk has been observed in many countries in the last few years due to the beneficial properties for human health attributed to A2 β-casein variant. Methods of varying complexity and different equipment requirements have been proposed for the determination of the β-casein genotype of individual cows. We proposed herein a modification of a previously patented method based on an amplification-created restriction site PCR followed by restriction fragment length polymorphism analysis. This method allows to identify and differentiate A2-like from A1-like β-casein variants, after differential endonuclease cleavage flanking the nucleotide that determines the amino acid at position 67 of β-casein. The advantages of this method are that it:
• enables to unequivocally score A2-like as well as A1-like β-casein variants,
• can be performed at low cost in simply equipped molecular biology laboratories, and
• can be scaled up to analyze hundreds of samples per day.
For these reasons, and based on the results obtained from the analysis carried out in this work, it showed to be a reliable method for the screening of herds to selective breeding of homozygous cows and bulls for A2 or A2-like alleles. |
| format | info:ar-repo/semantics/artículo |
| id | INTA14567 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA145672023-04-24T12:21:09Z Modification of a previously patented method to unequivocally score A2-like and A1-like bovine β-casein variants Raschia, Maria Agustina Caffaro, María Eugenia Rossi, Ursula Amaranta Poli, Mario Andres Ganado Bovino Métodos Caseína Beta-caseína Leche de Vaca Cattle Methods Casein Beta-casein Cow Milk PCR A growing interest in the production and commercialization of A2 cow's milk has been observed in many countries in the last few years due to the beneficial properties for human health attributed to A2 β-casein variant. Methods of varying complexity and different equipment requirements have been proposed for the determination of the β-casein genotype of individual cows. We proposed herein a modification of a previously patented method based on an amplification-created restriction site PCR followed by restriction fragment length polymorphism analysis. This method allows to identify and differentiate A2-like from A1-like β-casein variants, after differential endonuclease cleavage flanking the nucleotide that determines the amino acid at position 67 of β-casein. The advantages of this method are that it: • enables to unequivocally score A2-like as well as A1-like β-casein variants, • can be performed at low cost in simply equipped molecular biology laboratories, and • can be scaled up to analyze hundreds of samples per day. For these reasons, and based on the results obtained from the analysis carried out in this work, it showed to be a reliable method for the screening of herds to selective breeding of homozygous cows and bulls for A2 or A2-like alleles. Instituto de Genética Fil: Raschia, Maria Agustina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Caffaro, María Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Rossi, Ursula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Rossi, Ursula. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Poli, Mario Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina 2023-04-24T12:19:25Z 2023-04-24T12:19:25Z 2023-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/14567 https://www.sciencedirect.com/science/article/pii/S2215016123001838 2215-0161 https://doi.org/10.1016/j.mex.2023.102183 eng info:eu-repograntAgreement/INTA/2019-PE-E1-I018-001/2019-PE-E1-I018-001/AR./Crecimiento del rodeo lechero info:eu-repograntAgreement/INTA/2019-PE-E6-I114-001/2019-PE-E6-I114-001/AR./Caracterización de la diversidad genética de plantas, animales y microorganismos mediante herramientas de genómica aplicada. info:eu-repograntAgreement/INTA/2019-PE-E6-I145-001/2019-PE-E6-I145-001/AR./Mejora genética objetiva para aumentar la eficiencia de los sistemas de producción animal. info:eu-repograntAgreement/INTA/2019-PT-E6-I513-001/2019-PT-E6-I513-001/AR./Plataforma de mejoramiento animal info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier MethodsX 10 : 102183. (April 2023) |
| spellingShingle | Ganado Bovino Métodos Caseína Beta-caseína Leche de Vaca Cattle Methods Casein Beta-casein Cow Milk PCR Raschia, Maria Agustina Caffaro, María Eugenia Rossi, Ursula Amaranta Poli, Mario Andres Modification of a previously patented method to unequivocally score A2-like and A1-like bovine β-casein variants |
| title | Modification of a previously patented method to unequivocally score A2-like and A1-like bovine β-casein variants |
| title_full | Modification of a previously patented method to unequivocally score A2-like and A1-like bovine β-casein variants |
| title_fullStr | Modification of a previously patented method to unequivocally score A2-like and A1-like bovine β-casein variants |
| title_full_unstemmed | Modification of a previously patented method to unequivocally score A2-like and A1-like bovine β-casein variants |
| title_short | Modification of a previously patented method to unequivocally score A2-like and A1-like bovine β-casein variants |
| title_sort | modification of a previously patented method to unequivocally score a2 like and a1 like bovine β casein variants |
| topic | Ganado Bovino Métodos Caseína Beta-caseína Leche de Vaca Cattle Methods Casein Beta-casein Cow Milk PCR |
| url | http://hdl.handle.net/20.500.12123/14567 https://www.sciencedirect.com/science/article/pii/S2215016123001838 https://doi.org/10.1016/j.mex.2023.102183 |
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