A fijivirus major viroplasm protein shows RNA-stimulated ATPase activity by adopting pentameric and hexameric assemblies of dimers
Fijiviruses replicate and package their genomes within viroplasms in a pro cess involving RNA-RNA and RNA-protein interactions. Here, we demonstrate that the 24 C-terminal residues (C-arm) of the P9-1 major viroplasm protein of the mal de Río Cuarto virus (MRCV) are required for its multimerization...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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American Society for Microbiology
2023
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/14537 https://journals.asm.org/doi/10.1128/mbio.00023-23 https://doi.org/10.1128/mbio.00023-23 |
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| author | Llauger, Gabriela Melero, Roberto Monti, Demian Esteban Sycz, Gabriela Huck-Iriart, Cristian Cerutti, Maria Laura Klinke, Sebastián Mikkelsen, Evelyn Tijman, Ariel Arranz, Rocío Alfonso, Victoria Arellano, Sofía Maité Goldbaum, Fernando Alberto Sterckx, Yann G. J. Carazo, José María Kaufman, Sergio B. Dans, Pablo D. Del Vas, Mariana Otero, Lisandro H. |
| author_browse | Alfonso, Victoria Arellano, Sofía Maité Arranz, Rocío Carazo, José María Cerutti, Maria Laura Dans, Pablo D. Del Vas, Mariana Goldbaum, Fernando Alberto Huck-Iriart, Cristian Kaufman, Sergio B. Klinke, Sebastián Llauger, Gabriela Melero, Roberto Mikkelsen, Evelyn Monti, Demian Esteban Otero, Lisandro H. Sterckx, Yann G. J. Sycz, Gabriela Tijman, Ariel |
| author_facet | Llauger, Gabriela Melero, Roberto Monti, Demian Esteban Sycz, Gabriela Huck-Iriart, Cristian Cerutti, Maria Laura Klinke, Sebastián Mikkelsen, Evelyn Tijman, Ariel Arranz, Rocío Alfonso, Victoria Arellano, Sofía Maité Goldbaum, Fernando Alberto Sterckx, Yann G. J. Carazo, José María Kaufman, Sergio B. Dans, Pablo D. Del Vas, Mariana Otero, Lisandro H. |
| author_sort | Llauger, Gabriela |
| collection | INTA Digital |
| description | Fijiviruses replicate and package their genomes within viroplasms in a pro cess involving RNA-RNA and RNA-protein interactions. Here, we demonstrate that the 24 C-terminal residues (C-arm) of the P9-1 major viroplasm protein of the mal de Río Cuarto virus (MRCV) are required for its multimerization and the formation of viroplasm like structures. Using an integrative structural approach, the C-arm was found to be dis pensable for P9-1 dimer assembly but essential for the formation of pentamers and hexamers of dimers (decamers and dodecamers), which favored RNA binding. Although both P9-1 and P9-1DC-arm catalyzed ATP with similar activities, an RNA-stimulated ATPase activity was only detected in the full-length protein, indicating a C-arm-mediated interaction between the ATP catalytic site and the allosteric RNA binding sites in the (do)decameric assemblies. A stronger preference to bind phosphate moieties in the dec amer was predicted, suggesting that the allosteric modulation of ATPase activity by RNA is favored in this structural conformation. Our work reveals the structural versatility of a fijivirus major viroplasm protein and provides clues to its mechanism of action. |
| format | info:ar-repo/semantics/artículo |
| id | INTA14537 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | American Society for Microbiology |
| publisherStr | American Society for Microbiology |
| record_format | dspace |
| spelling | INTA145372023-04-21T10:10:27Z A fijivirus major viroplasm protein shows RNA-stimulated ATPase activity by adopting pentameric and hexameric assemblies of dimers Llauger, Gabriela Melero, Roberto Monti, Demian Esteban Sycz, Gabriela Huck-Iriart, Cristian Cerutti, Maria Laura Klinke, Sebastián Mikkelsen, Evelyn Tijman, Ariel Arranz, Rocío Alfonso, Victoria Arellano, Sofía Maité Goldbaum, Fernando Alberto Sterckx, Yann G. J. Carazo, José María Kaufman, Sergio B. Dans, Pablo D. Del Vas, Mariana Otero, Lisandro H. Plant Viruses Reovirus dsRNA viruses ATPase Fijivirus Virus de las Plantas ATPasa Mal de Río Cuarto virus Virus del Mal de Río Cuarto Fijiviruses replicate and package their genomes within viroplasms in a pro cess involving RNA-RNA and RNA-protein interactions. Here, we demonstrate that the 24 C-terminal residues (C-arm) of the P9-1 major viroplasm protein of the mal de Río Cuarto virus (MRCV) are required for its multimerization and the formation of viroplasm like structures. Using an integrative structural approach, the C-arm was found to be dis pensable for P9-1 dimer assembly but essential for the formation of pentamers and hexamers of dimers (decamers and dodecamers), which favored RNA binding. Although both P9-1 and P9-1DC-arm catalyzed ATP with similar activities, an RNA-stimulated ATPase activity was only detected in the full-length protein, indicating a C-arm-mediated interaction between the ATP catalytic site and the allosteric RNA binding sites in the (do)decameric assemblies. A stronger preference to bind phosphate moieties in the dec amer was predicted, suggesting that the allosteric modulation of ATPase activity by RNA is favored in this structural conformation. Our work reveals the structural versatility of a fijivirus major viroplasm protein and provides clues to its mechanism of action. Instituto de Biotecnología Fil: Llauger, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Llauger, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Melero, Roberto. Consejo Superior de Investigaciones Científicas (CSIC). Centro Nacional de Biotecnología. Biocomputing Unit. Campus Universidad Autónoma de Madrid; España Fil: Monti, Demian Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Monti, Demian Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Sycz, Gabriela. Fundación Instituto Leloir; Argentina Fil: Sycz, Gabriela. Instituto de Investigaciones Bioquímicas de Buenos Aires (IIBBA); Argentina Fil: Sycz, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Huck-Iriart, Cristian. Universidad Nacional de San Martín. Instituto de Tecnologías Emergentes y Ciencias Aplicadas (ITECA); Argentina Fil: Huck-Iriart, Cristian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cerutti, Maria Laura. Fundación Instituto Leloir; Argentina Fil: Cerutti, Maria Laura. Instituto de Investigaciones Bioquímicas de Buenos Aires (IIBBA); Argentina Fil: Cerutti, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cerutti, Maria Laura. Plataforma Argentina de Biología Estructural y Metabolómica (PLABEM); Argentina Fil: Klinke, Sebastián. Fundación Instituto Leloir; Argentina Fil: Klinke, Sebastián. Instituto de Investigaciones Bioquímicas de Buenos Aires (IIBBA); Argentina Fil: Klinke, Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Klinke, Sebastián. Plataforma Argentina de Biología Estructural y Metabolómica (PLABEM); Argentina Fil: Mikkelsen, Evelyn. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Fisicoquímica Biológicas (IQUIFIB). Departamento de Química Biológica; Argentina Fil: Mikkelsen, Evelyn. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Tijman, Ariel. Universidad de la República (UdelaR). Department of Biological Sciences. Computational Biophysics Lab; Uruguay Fil: Tijman, Ariel. Universidad de la República (UdelaR). Facultad de Química. Bioscience Department. Molecular Microbiology Lab; Uruguay Fil: Tijman, Ariel. Universidad de la República (UdelaR). Facultad de Química. Organic Chemistry Department and Bioscience Department. Biocatalysis and Biotransformation Lab; Uruguay Fil: Arranz, Rocío. Universidad Autónoma de Madrid. Centro Nacional de Biotecnología. Departamento de Estructura de Macromoléculas; España Fil: Arranz, Rocío. Consejo Superior de Investigaciones Científicas (CSIC); España Fil: Alfonso, Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Alfonso, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Arellano, Sofía Maité. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Arellano, Sofía Maité. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Goldbaum, Fernando Alberto. Fundación Instituto Leloir; Argentina Fil: Goldbaum, Fernando Alberto. Instituto de Investigaciones Bioquímicas de Buenos Aires (IIBBA); Argentina Fil: Goldbaum, Fernando Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Goldbaum, Fernando Alberto. Plataforma Argentina de Biología Estructural y Metabolómica (PLABEM); Argentina Fil: Sterckx, Yann G. J. University of Antwerp. Laboratory of Medical Biochemistry and the Infla-Med; Bélgica Fil: Carazo, José María. Consejo Superior de Investigaciones Científicas (CSIC). Centro Nacional de Biotecnología. Biocomputing Unit. Campus Universidad Autónoma de Madrid; España Fil: Kaufman, Sergio B. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Fisicoquímica Biológicas (IQUIFIB). Departamento de Química Biológica; Argentina Fil: Kaufman, Sergio B. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Dans, Pablo D. Universidad de la República (UdelaR). Department of Biological Sciences. Computational Biophysics Lab; Uruguay Fil: Dans, Pablo D. Institut Pasteur de Montevideo. Bioinformatics Unit; Uruguay Fil: Dans, Pablo D. Institute for Research in Biomedicine. Molecular Modelling and Bioinformatics Group; España Fil: Del Vas, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Del Vas, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Otero, Lisandro H. Fundación Instituto Leloir; Argentina Fil: Otero, Lisandro H. Instituto de Investigaciones Bioquímicas de Buenos Aires (IIBBA); Argentina Fil: Otero, Lisandro H. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Otero, Lisandro H. Plataforma Argentina de Biología Estructural y Metabolómica (PLABEM); Argentina 2023-04-21T10:02:20Z 2023-04-21T10:02:20Z 2023-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/14537 https://journals.asm.org/doi/10.1128/mbio.00023-23 2150-7511 2161-2129 https://doi.org/10.1128/mbio.00023-23 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf American Society for Microbiology mBio : 1-24 (14 February 2023) |
| spellingShingle | Plant Viruses Reovirus dsRNA viruses ATPase Fijivirus Virus de las Plantas ATPasa Mal de Río Cuarto virus Virus del Mal de Río Cuarto Llauger, Gabriela Melero, Roberto Monti, Demian Esteban Sycz, Gabriela Huck-Iriart, Cristian Cerutti, Maria Laura Klinke, Sebastián Mikkelsen, Evelyn Tijman, Ariel Arranz, Rocío Alfonso, Victoria Arellano, Sofía Maité Goldbaum, Fernando Alberto Sterckx, Yann G. J. Carazo, José María Kaufman, Sergio B. Dans, Pablo D. Del Vas, Mariana Otero, Lisandro H. A fijivirus major viroplasm protein shows RNA-stimulated ATPase activity by adopting pentameric and hexameric assemblies of dimers |
| title | A fijivirus major viroplasm protein shows RNA-stimulated ATPase activity by adopting pentameric and hexameric assemblies of dimers |
| title_full | A fijivirus major viroplasm protein shows RNA-stimulated ATPase activity by adopting pentameric and hexameric assemblies of dimers |
| title_fullStr | A fijivirus major viroplasm protein shows RNA-stimulated ATPase activity by adopting pentameric and hexameric assemblies of dimers |
| title_full_unstemmed | A fijivirus major viroplasm protein shows RNA-stimulated ATPase activity by adopting pentameric and hexameric assemblies of dimers |
| title_short | A fijivirus major viroplasm protein shows RNA-stimulated ATPase activity by adopting pentameric and hexameric assemblies of dimers |
| title_sort | fijivirus major viroplasm protein shows rna stimulated atpase activity by adopting pentameric and hexameric assemblies of dimers |
| topic | Plant Viruses Reovirus dsRNA viruses ATPase Fijivirus Virus de las Plantas ATPasa Mal de Río Cuarto virus Virus del Mal de Río Cuarto |
| url | http://hdl.handle.net/20.500.12123/14537 https://journals.asm.org/doi/10.1128/mbio.00023-23 https://doi.org/10.1128/mbio.00023-23 |
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