Molecular characterization of yerba mate chlorosis-associated virus, a putative cytorhabdvirus infecting yerba mate (Ilex paraguariensis)
We present the molecular characterization of a new virus infecting yerba mate (Ilex paraguariensis St. Hil.) in Argentina. Deep sequencing of diseased yerba mate plants showing chlorotic linear patterns, chlorotic rings, and vein yellowing resulted in the identification of a new virus resembling pla...
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| Format: | Artículo |
| Language: | Inglés |
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2017
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| Online Access: | http://hdl.handle.net/20.500.12123/1453 https://link.springer.com/article/10.1007%2Fs00705-017-3363-8 https://doi.org/10.1007/s00705-017-3363-8 |
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| author | Bejerman, Nicolas Esteban De Breuil, Soledad Debat, Humberto Julio Miretti, Marcos Badaracco, Alejandra Nome Docampo, Claudia |
| author_browse | Badaracco, Alejandra Bejerman, Nicolas Esteban De Breuil, Soledad Debat, Humberto Julio Miretti, Marcos Nome Docampo, Claudia |
| author_facet | Bejerman, Nicolas Esteban De Breuil, Soledad Debat, Humberto Julio Miretti, Marcos Badaracco, Alejandra Nome Docampo, Claudia |
| author_sort | Bejerman, Nicolas Esteban |
| collection | INTA Digital |
| description | We present the molecular characterization of a new virus infecting yerba mate (Ilex paraguariensis St. Hil.) in Argentina. Deep sequencing of diseased yerba mate plants showing chlorotic linear patterns, chlorotic rings, and vein yellowing resulted in the identification of a new virus resembling plant rhabdoviruses in sequence and genome structure. We have determined the complete genome sequence of this virus, which is 12,876 nt long. Seven open reading frames (ORFs) were identified in the antigenomic orientation of the negative-sense, single-stranded viral RNA, in the order 3′-N-P-P3-P4-M-G-L-5′. Phylogenetic analysis suggested that the described virus is a new member of the genus Cytorhabdovirus, which was supported by the observation of rhabdovirus-like particles within the cytoplasm of infected yerba mate cells. The virus has been tentatively named “yerba mate chlorosis-associated virus” (YmCaV). The availability of the YmCaV genome sequence will contribute to assessing the genetic variability of this virus and determining its role in this yerba mate disease. |
| format | Artículo |
| id | INTA1453 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA14532019-03-22T15:05:54Z Molecular characterization of yerba mate chlorosis-associated virus, a putative cytorhabdvirus infecting yerba mate (Ilex paraguariensis) Bejerman, Nicolas Esteban De Breuil, Soledad Debat, Humberto Julio Miretti, Marcos Badaracco, Alejandra Nome Docampo, Claudia Ilex Paraguariensis Virus de las Plantas Clorosis Genética Molecular Plant Viruses Chlorosis Molecular Genetics Yerba Mate We present the molecular characterization of a new virus infecting yerba mate (Ilex paraguariensis St. Hil.) in Argentina. Deep sequencing of diseased yerba mate plants showing chlorotic linear patterns, chlorotic rings, and vein yellowing resulted in the identification of a new virus resembling plant rhabdoviruses in sequence and genome structure. We have determined the complete genome sequence of this virus, which is 12,876 nt long. Seven open reading frames (ORFs) were identified in the antigenomic orientation of the negative-sense, single-stranded viral RNA, in the order 3′-N-P-P3-P4-M-G-L-5′. Phylogenetic analysis suggested that the described virus is a new member of the genus Cytorhabdovirus, which was supported by the observation of rhabdovirus-like particles within the cytoplasm of infected yerba mate cells. The virus has been tentatively named “yerba mate chlorosis-associated virus” (YmCaV). The availability of the YmCaV genome sequence will contribute to assessing the genetic variability of this virus and determining its role in this yerba mate disease. Inst. Patología Vegetal Fil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: De Breuil, Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Debat, Humberto Julio Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Fil: Miretti, Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Badaracco, Alejandra. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Nome Docampo, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina 2017-10-11T11:43:09Z 2017-10-11T11:43:09Z 2017-08 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/1453 https://link.springer.com/article/10.1007%2Fs00705-017-3363-8 0304-8608 (Print) 1432-8798 (Online) https://doi.org/10.1007/s00705-017-3363-8 eng info:eu-repo/semantics/restrictedAccess application/pdf Archives of virology 162 (8) : 2481–2484. (August 2017) |
| spellingShingle | Ilex Paraguariensis Virus de las Plantas Clorosis Genética Molecular Plant Viruses Chlorosis Molecular Genetics Yerba Mate Bejerman, Nicolas Esteban De Breuil, Soledad Debat, Humberto Julio Miretti, Marcos Badaracco, Alejandra Nome Docampo, Claudia Molecular characterization of yerba mate chlorosis-associated virus, a putative cytorhabdvirus infecting yerba mate (Ilex paraguariensis) |
| title | Molecular characterization of yerba mate chlorosis-associated virus, a putative cytorhabdvirus infecting yerba mate (Ilex paraguariensis) |
| title_full | Molecular characterization of yerba mate chlorosis-associated virus, a putative cytorhabdvirus infecting yerba mate (Ilex paraguariensis) |
| title_fullStr | Molecular characterization of yerba mate chlorosis-associated virus, a putative cytorhabdvirus infecting yerba mate (Ilex paraguariensis) |
| title_full_unstemmed | Molecular characterization of yerba mate chlorosis-associated virus, a putative cytorhabdvirus infecting yerba mate (Ilex paraguariensis) |
| title_short | Molecular characterization of yerba mate chlorosis-associated virus, a putative cytorhabdvirus infecting yerba mate (Ilex paraguariensis) |
| title_sort | molecular characterization of yerba mate chlorosis associated virus a putative cytorhabdvirus infecting yerba mate ilex paraguariensis |
| topic | Ilex Paraguariensis Virus de las Plantas Clorosis Genética Molecular Plant Viruses Chlorosis Molecular Genetics Yerba Mate |
| url | http://hdl.handle.net/20.500.12123/1453 https://link.springer.com/article/10.1007%2Fs00705-017-3363-8 https://doi.org/10.1007/s00705-017-3363-8 |
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