Engineering Pseudomonas protegens Pf-5 for nitrogen fixation and its application to improve plant growth under nitrogen-deficient conditions
Nitrogen is the second most critical factor for crop production after water. In this study, the beneficial rhizobacterium Pseudomonas protegens Pf-5 was genetically modified to fix nitrogen using the genes encoding the nitrogenase of Pseudomonas stutzeri A1501 via the X940 cosmid. Pf-5 X940 was able...
| Autores principales: | , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/1450 http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0063666 https://doi.org/10.1371/journal.pone.0063666 |
| _version_ | 1855482890176954368 |
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| author | Setten, Lorena Soto, Gabriela Cinthia Mozzicafreddo, Matteo Fox, Ana Romina Lisi, Christian Daniel Cuccioloni, Massimiliano Angeletti, Mauro Pagano, Elba Maria Diaz Paleo, Antonio Horacio Ayub, Nicolás Daniel |
| author_browse | Angeletti, Mauro Ayub, Nicolás Daniel Cuccioloni, Massimiliano Diaz Paleo, Antonio Horacio Fox, Ana Romina Lisi, Christian Daniel Mozzicafreddo, Matteo Pagano, Elba Maria Setten, Lorena Soto, Gabriela Cinthia |
| author_facet | Setten, Lorena Soto, Gabriela Cinthia Mozzicafreddo, Matteo Fox, Ana Romina Lisi, Christian Daniel Cuccioloni, Massimiliano Angeletti, Mauro Pagano, Elba Maria Diaz Paleo, Antonio Horacio Ayub, Nicolás Daniel |
| author_sort | Setten, Lorena |
| collection | INTA Digital |
| description | Nitrogen is the second most critical factor for crop production after water. In this study, the beneficial rhizobacterium Pseudomonas protegens Pf-5 was genetically modified to fix nitrogen using the genes encoding the nitrogenase of Pseudomonas stutzeri A1501 via the X940 cosmid. Pf-5 X940 was able to grow in L medium without nitrogen, displayed high nitrogenase activity and released significant quantities of ammonium to the medium. Pf-5 X940 also showed constitutive expression and enzymatic activity of nitrogenase in ammonium medium or in nitrogen-free medium, suggesting a constitutive nitrogen fixation. Similar to Pseudomonas protegens Pf-5, Pseudomonas putida, Pseudomonas veronii and Pseudomonas taetrolens but not Pseudomonas balearica and Pseudomonas stutzeri transformed with cosmid X940 showed constitutive nitrogenase activity and high ammonium production, suggesting that this phenotype depends on the genome context and that this technology to obtain nitrogen-fixing bacteria is not restricted to Pf-5. Interestingly, inoculation of Arabidopsis, alfalfa, tall fescue and maize with Pf-5 X940 increased the ammonium concentration in soil and plant productivity under nitrogen-deficient conditions. In conclusion, these results open the way to the production of effective recombinant inoculants for nitrogen fixation on a wide range of crops. |
| format | Artículo |
| id | INTA1450 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA14502017-12-27T14:10:52Z Engineering Pseudomonas protegens Pf-5 for nitrogen fixation and its application to improve plant growth under nitrogen-deficient conditions Setten, Lorena Soto, Gabriela Cinthia Mozzicafreddo, Matteo Fox, Ana Romina Lisi, Christian Daniel Cuccioloni, Massimiliano Angeletti, Mauro Pagano, Elba Maria Diaz Paleo, Antonio Horacio Ayub, Nicolás Daniel Pseudomonas Microorganismos Organismos Modificados Genéticamente Bacteria Fijadora del Nitrógeno Fijación del Nitrógeno Fijación del Amonio Nitrogenasa Rizosfera Nitrógeno Asimilable Inoculación Inoculation Arabidopsis Biotecnología Vegetal Sustancias de Crecimiento Vegetal Deficiencias del Suelo Microorganisms Genetically Modified Organisms Nitrogen Fixing Bacteria Nitrogen Fixation Ammonium Fixation Nitrogenase Rhizosphere Digestible Nitrogen Plant Biotechnology Soil Deficiencies Argentina Diazotrofos Endofitos Bacterias Recombinantes Pseudomonas Protogens Pf5 Fijación Biológica del Nitrógeno Deficiencias de Nitrógeno Nitrogen is the second most critical factor for crop production after water. In this study, the beneficial rhizobacterium Pseudomonas protegens Pf-5 was genetically modified to fix nitrogen using the genes encoding the nitrogenase of Pseudomonas stutzeri A1501 via the X940 cosmid. Pf-5 X940 was able to grow in L medium without nitrogen, displayed high nitrogenase activity and released significant quantities of ammonium to the medium. Pf-5 X940 also showed constitutive expression and enzymatic activity of nitrogenase in ammonium medium or in nitrogen-free medium, suggesting a constitutive nitrogen fixation. Similar to Pseudomonas protegens Pf-5, Pseudomonas putida, Pseudomonas veronii and Pseudomonas taetrolens but not Pseudomonas balearica and Pseudomonas stutzeri transformed with cosmid X940 showed constitutive nitrogenase activity and high ammonium production, suggesting that this phenotype depends on the genome context and that this technology to obtain nitrogen-fixing bacteria is not restricted to Pf-5. Interestingly, inoculation of Arabidopsis, alfalfa, tall fescue and maize with Pf-5 X940 increased the ammonium concentration in soil and plant productivity under nitrogen-deficient conditions. In conclusion, these results open the way to the production of effective recombinant inoculants for nitrogen fixation on a wide range of crops. Fil: Setten, Lorena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina Fil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Mozzicafreddo, Matteo. Università di Camerino. School of Biosciences and Biotechnology; Italia Fil: Fox, Ana Romina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Lisi, Christian Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina Fil: Cuccioloni, Massimiliano. Università di Camerino. School of Biosciences and Biotechnology; Italia Fil: Angeletti, Mauro. Università di Camerino. School of Biosciences and Biotechnology; Italia Fil: Pagano, Elba Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina Fil: Diaz Paleo, Antonio Horacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2017-10-10T14:32:22Z 2017-10-10T14:32:22Z 2013-05-13 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/1450 http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0063666 1932-6203 https://doi.org/10.1371/journal.pone.0063666 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf PLoS ONE 8 (5) : e63666. (2013) |
| spellingShingle | Pseudomonas Microorganismos Organismos Modificados Genéticamente Bacteria Fijadora del Nitrógeno Fijación del Nitrógeno Fijación del Amonio Nitrogenasa Rizosfera Nitrógeno Asimilable Inoculación Inoculation Arabidopsis Biotecnología Vegetal Sustancias de Crecimiento Vegetal Deficiencias del Suelo Microorganisms Genetically Modified Organisms Nitrogen Fixing Bacteria Nitrogen Fixation Ammonium Fixation Nitrogenase Rhizosphere Digestible Nitrogen Plant Biotechnology Soil Deficiencies Argentina Diazotrofos Endofitos Bacterias Recombinantes Pseudomonas Protogens Pf5 Fijación Biológica del Nitrógeno Deficiencias de Nitrógeno Setten, Lorena Soto, Gabriela Cinthia Mozzicafreddo, Matteo Fox, Ana Romina Lisi, Christian Daniel Cuccioloni, Massimiliano Angeletti, Mauro Pagano, Elba Maria Diaz Paleo, Antonio Horacio Ayub, Nicolás Daniel Engineering Pseudomonas protegens Pf-5 for nitrogen fixation and its application to improve plant growth under nitrogen-deficient conditions |
| title | Engineering Pseudomonas protegens Pf-5 for nitrogen fixation and its application to improve plant growth under nitrogen-deficient conditions |
| title_full | Engineering Pseudomonas protegens Pf-5 for nitrogen fixation and its application to improve plant growth under nitrogen-deficient conditions |
| title_fullStr | Engineering Pseudomonas protegens Pf-5 for nitrogen fixation and its application to improve plant growth under nitrogen-deficient conditions |
| title_full_unstemmed | Engineering Pseudomonas protegens Pf-5 for nitrogen fixation and its application to improve plant growth under nitrogen-deficient conditions |
| title_short | Engineering Pseudomonas protegens Pf-5 for nitrogen fixation and its application to improve plant growth under nitrogen-deficient conditions |
| title_sort | engineering pseudomonas protegens pf 5 for nitrogen fixation and its application to improve plant growth under nitrogen deficient conditions |
| topic | Pseudomonas Microorganismos Organismos Modificados Genéticamente Bacteria Fijadora del Nitrógeno Fijación del Nitrógeno Fijación del Amonio Nitrogenasa Rizosfera Nitrógeno Asimilable Inoculación Inoculation Arabidopsis Biotecnología Vegetal Sustancias de Crecimiento Vegetal Deficiencias del Suelo Microorganisms Genetically Modified Organisms Nitrogen Fixing Bacteria Nitrogen Fixation Ammonium Fixation Nitrogenase Rhizosphere Digestible Nitrogen Plant Biotechnology Soil Deficiencies Argentina Diazotrofos Endofitos Bacterias Recombinantes Pseudomonas Protogens Pf5 Fijación Biológica del Nitrógeno Deficiencias de Nitrógeno |
| url | http://hdl.handle.net/20.500.12123/1450 http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0063666 https://doi.org/10.1371/journal.pone.0063666 |
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