CRISPR/Cas9-mediated knockout of a polyester synthase-like gene delays flowering time in alfalfa
Alfalfa (Medicago sativa), commonly known as the “Queen of Forages”, is the main source of plant protein for meat and milk production systems worldwide. Since alfalfa is a perennial vegetative-harvest forage, delaying flowering is a promising strategy to increase alfalfa biomass yield and quality (A...
| Autores principales: | , , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2023
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/14474 https://link.springer.com/article/10.1007/s00299-023-02997-9 https://doi.org/10.1007/s00299-023-02997-9 |
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| author | Galindo Sotomonte, Luisa Fernanda Jozefkowicz, Cintia Gomez, Maria Cristina Stritzler, Margarita Frare, Romina Alejandra Bottero, Ana Emilia Tajima, Hiromi Blumwald, Eduardo Ayub, Nicolás Daniel Soto, Gabriela Cynthia |
| author_browse | Ayub, Nicolás Daniel Blumwald, Eduardo Bottero, Ana Emilia Frare, Romina Alejandra Galindo Sotomonte, Luisa Fernanda Gomez, Maria Cristina Jozefkowicz, Cintia Soto, Gabriela Cynthia Stritzler, Margarita Tajima, Hiromi |
| author_facet | Galindo Sotomonte, Luisa Fernanda Jozefkowicz, Cintia Gomez, Maria Cristina Stritzler, Margarita Frare, Romina Alejandra Bottero, Ana Emilia Tajima, Hiromi Blumwald, Eduardo Ayub, Nicolás Daniel Soto, Gabriela Cynthia |
| author_sort | Galindo Sotomonte, Luisa Fernanda |
| collection | INTA Digital |
| description | Alfalfa (Medicago sativa), commonly known as the “Queen of Forages”, is the main source of plant protein for meat and milk production systems worldwide. Since alfalfa is a perennial vegetative-harvest forage, delaying flowering is a promising strategy to increase alfalfa biomass yield and quality (Adhikari et al. 2019). The production of transgenic alfalfa either overexpressing or underexpressing different genes has led to delayed flowering phenotypes (Lorenzo et al. 2020; Ma et al. 2021). Unfortunately, the deregulation of transgenic events involves long-term and expensive procedures that limit their commercial utilization. The recently described increased efficiency of the CRISPR/Cas9 system in regenerative alfalfa allowed full allelic knockout of target genes, and the production of edited alfalfa plants (Stritzler et al. 2022), opening the way for a new generation of transgene-free alfalfa varieties displaying delayed flowering. |
| format | info:ar-repo/semantics/artículo |
| id | INTA14474 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA144742023-04-13T17:23:48Z CRISPR/Cas9-mediated knockout of a polyester synthase-like gene delays flowering time in alfalfa Galindo Sotomonte, Luisa Fernanda Jozefkowicz, Cintia Gomez, Maria Cristina Stritzler, Margarita Frare, Romina Alejandra Bottero, Ana Emilia Tajima, Hiromi Blumwald, Eduardo Ayub, Nicolás Daniel Soto, Gabriela Cynthia Medicago sativa Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Interespaciadas Floración Genética CRISPR Flowering Stage Flowering Genetics Arabidopsis Alfalfa Lucerne Alfalfa (Medicago sativa), commonly known as the “Queen of Forages”, is the main source of plant protein for meat and milk production systems worldwide. Since alfalfa is a perennial vegetative-harvest forage, delaying flowering is a promising strategy to increase alfalfa biomass yield and quality (Adhikari et al. 2019). The production of transgenic alfalfa either overexpressing or underexpressing different genes has led to delayed flowering phenotypes (Lorenzo et al. 2020; Ma et al. 2021). Unfortunately, the deregulation of transgenic events involves long-term and expensive procedures that limit their commercial utilization. The recently described increased efficiency of the CRISPR/Cas9 system in regenerative alfalfa allowed full allelic knockout of target genes, and the production of edited alfalfa plants (Stritzler et al. 2022), opening the way for a new generation of transgene-free alfalfa varieties displaying delayed flowering. Instituto de Biotecnología Fil: Galindo Sotomonte, Luisa Fernanda. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Galindo Sotomonte, Luisa Fernanda. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Galindo Sotomonte, Luisa Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Jozefkowicz, Cintia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Jozefkowicz, Cintia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Jozefkowicz, Cintia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gomez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Gomez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Gomez, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Stritzler, Margarita. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Stritzler, Margarita. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Stritzler, Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Frare, Romina Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Frare, Romina Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Frare, Romina Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bottero, Ana Emilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Bottero, Ana Emilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Bottero, Ana Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Tajima, Hiromi. University of California, Davis. Department of Plant Sciences; Estados Unidos Fil: Blumwald, Eduardo. University of California, Davis. Department of Plant Sciences; Estados Unidos Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Ayub, Nicolás Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Soto, Gabriela Cynthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Soto, Gabriela Cynthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Soto, Gabriela Cynthia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2023-04-13T17:20:52Z 2023-04-13T17:20:52Z 2023-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/14474 https://link.springer.com/article/10.1007/s00299-023-02997-9 0721-7714 1432-203X https://doi.org/10.1007/s00299-023-02997-9 eng info:eu-repograntAgreement/INTA/2019-PD-E6-I116-001/2019-PD-E6-I116-001/AR./Identificación y análisis funcional de genes o redes génicas de interés biotecnológico con fin agropecuario, forestal, agroalimentario y/o agroindustrial. info:eu-repograntAgreement/INTA/2019-PD-E4-I085-001/2019-PD-E4-I085-001/AR./Determinación de los mecanismos de resistencia a enfermedades mediante la caracterización de las interacciones moleculares en sistemas planta-patógeno. info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Plant Cell Reports : 1-4 (2023) |
| spellingShingle | Medicago sativa Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Interespaciadas Floración Genética CRISPR Flowering Stage Flowering Genetics Arabidopsis Alfalfa Lucerne Galindo Sotomonte, Luisa Fernanda Jozefkowicz, Cintia Gomez, Maria Cristina Stritzler, Margarita Frare, Romina Alejandra Bottero, Ana Emilia Tajima, Hiromi Blumwald, Eduardo Ayub, Nicolás Daniel Soto, Gabriela Cynthia CRISPR/Cas9-mediated knockout of a polyester synthase-like gene delays flowering time in alfalfa |
| title | CRISPR/Cas9-mediated knockout of a polyester synthase-like gene delays flowering time in alfalfa |
| title_full | CRISPR/Cas9-mediated knockout of a polyester synthase-like gene delays flowering time in alfalfa |
| title_fullStr | CRISPR/Cas9-mediated knockout of a polyester synthase-like gene delays flowering time in alfalfa |
| title_full_unstemmed | CRISPR/Cas9-mediated knockout of a polyester synthase-like gene delays flowering time in alfalfa |
| title_short | CRISPR/Cas9-mediated knockout of a polyester synthase-like gene delays flowering time in alfalfa |
| title_sort | crispr cas9 mediated knockout of a polyester synthase like gene delays flowering time in alfalfa |
| topic | Medicago sativa Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Interespaciadas Floración Genética CRISPR Flowering Stage Flowering Genetics Arabidopsis Alfalfa Lucerne |
| url | http://hdl.handle.net/20.500.12123/14474 https://link.springer.com/article/10.1007/s00299-023-02997-9 https://doi.org/10.1007/s00299-023-02997-9 |
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