Comparative Degradome Analysis of the Bovine Piroplasmid Pathogens Babesia bovis and Theileria annulata
Babesia bovis and Theileria annulata are tick-borne hemoprotozoans that impact bovine health and are responsible for considerable fatalities in tropical and subtropical regions around the world. Both pathogens infect the same vertebrate host, are closely related, and contain similar-sized genomes; h...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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MDPI
2023
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| Online Access: | http://hdl.handle.net/20.500.12123/14467 https://www.mdpi.com/2076-0817/12/2/237 https://doi.org/10.3390/pathogens12020237 |
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| author | Poklepovich Caride, Tomás Javier Mesplet, Maria Gallenti, Romina Josefina Florin-Christensen, Mónica Schnittger, Leonhard |
| author_browse | Florin-Christensen, Mónica Gallenti, Romina Josefina Mesplet, Maria Poklepovich Caride, Tomás Javier Schnittger, Leonhard |
| author_facet | Poklepovich Caride, Tomás Javier Mesplet, Maria Gallenti, Romina Josefina Florin-Christensen, Mónica Schnittger, Leonhard |
| author_sort | Poklepovich Caride, Tomás Javier |
| collection | INTA Digital |
| description | Babesia bovis and Theileria annulata are tick-borne hemoprotozoans that impact bovine health and are responsible for considerable fatalities in tropical and subtropical regions around the world. Both pathogens infect the same vertebrate host, are closely related, and contain similar-sized genomes; however, they differ in invertebrate host specificity, absence vs. presence of a schizont stage, erythrocyte invasion mechanism, and transovarial vs. transstadial transmission. Phylogenetic analysis and bidirectional best hit (BBH) identified a similar number of aspartic, metallo, and threonine proteinases and nonproteinase homologs. In contrast, a considerably increased number of S54 serine rhomboid proteinases and S9 nonproteinase homologs were identified in B. bovis, whereas C1A cysteine proteinases and A1 aspartic nonproteinase homologs were found to be expanded in T. annulata. Furthermore, a single proteinase of families S8 (subtilisin-like protein) and C12 (ubiquitin carboxyl-terminal hydrolase), as well as four nonproteinase homologs, one with dual domains M23-M23 and three with S9-S9, were exclusively present in B. bovis. Finally, a pronounced difference in species-specific ancillary domains was observed between both species. We hypothesize that the observed degradome differences represent functional correlates of the dissimilar life history features of B. bovis and T. annulata. The presented improved classification of piroplasmid proteinases will facilitate an informed choice for future in-depth functional studies. |
| format | info:ar-repo/semantics/artículo |
| id | INTA14467 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INTA144672023-04-13T14:13:50Z Comparative Degradome Analysis of the Bovine Piroplasmid Pathogens Babesia bovis and Theileria annulata Poklepovich Caride, Tomás Javier Mesplet, Maria Gallenti, Romina Josefina Florin-Christensen, Mónica Schnittger, Leonhard Babesia bovis Theileria annulata Enfermedades de los Animales Organismos Patógenos Ganado Bovino Animal Diseases Pathogens Cattle Babesia bovis and Theileria annulata are tick-borne hemoprotozoans that impact bovine health and are responsible for considerable fatalities in tropical and subtropical regions around the world. Both pathogens infect the same vertebrate host, are closely related, and contain similar-sized genomes; however, they differ in invertebrate host specificity, absence vs. presence of a schizont stage, erythrocyte invasion mechanism, and transovarial vs. transstadial transmission. Phylogenetic analysis and bidirectional best hit (BBH) identified a similar number of aspartic, metallo, and threonine proteinases and nonproteinase homologs. In contrast, a considerably increased number of S54 serine rhomboid proteinases and S9 nonproteinase homologs were identified in B. bovis, whereas C1A cysteine proteinases and A1 aspartic nonproteinase homologs were found to be expanded in T. annulata. Furthermore, a single proteinase of families S8 (subtilisin-like protein) and C12 (ubiquitin carboxyl-terminal hydrolase), as well as four nonproteinase homologs, one with dual domains M23-M23 and three with S9-S9, were exclusively present in B. bovis. Finally, a pronounced difference in species-specific ancillary domains was observed between both species. We hypothesize that the observed degradome differences represent functional correlates of the dissimilar life history features of B. bovis and T. annulata. The presented improved classification of piroplasmid proteinases will facilitate an informed choice for future in-depth functional studies. Instituto de Patobiologia Fil: Poklepovich Caride, Tomás Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Poklepovich Caride, Tomás Javier. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Mesplet, Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Mesplet, Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gallenti, Romina Josefina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Gallenti, Romina Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Florin-Christensen, Monica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Florin-Christensen, Monica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Schnittger, Leonhard. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2023-04-13T13:42:24Z 2023-04-13T13:42:24Z 2023-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/14467 https://www.mdpi.com/2076-0817/12/2/237 2076-0817 https://doi.org/10.3390/pathogens12020237 eng info:eu-repograntAgreement/INTA/2019-PD-E5-I105-001/2019-PD-E5-I105-001/AR./Patógenos animales: su interacción con el hospedador y el medio ambiente. Impacto en productividad, ecosistemas, sanidad animal y salud pública en el marco “Una Salud” info:eu-repograntAgreement/INTA/2019-PE-E5-I109-001/2019-PE-E5-I109-001/AR./Convocatoria: Estudios para el control de enfermedades subtropicales y/o transmitidas por vectores (Tristeza Bovina, Garrapatas, Miasis, Tripanosomiasis, Lengua Azul y la info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Pathogens 12 (2) : 237 (February 2023) |
| spellingShingle | Babesia bovis Theileria annulata Enfermedades de los Animales Organismos Patógenos Ganado Bovino Animal Diseases Pathogens Cattle Poklepovich Caride, Tomás Javier Mesplet, Maria Gallenti, Romina Josefina Florin-Christensen, Mónica Schnittger, Leonhard Comparative Degradome Analysis of the Bovine Piroplasmid Pathogens Babesia bovis and Theileria annulata |
| title | Comparative Degradome Analysis of the Bovine Piroplasmid Pathogens Babesia bovis and Theileria annulata |
| title_full | Comparative Degradome Analysis of the Bovine Piroplasmid Pathogens Babesia bovis and Theileria annulata |
| title_fullStr | Comparative Degradome Analysis of the Bovine Piroplasmid Pathogens Babesia bovis and Theileria annulata |
| title_full_unstemmed | Comparative Degradome Analysis of the Bovine Piroplasmid Pathogens Babesia bovis and Theileria annulata |
| title_short | Comparative Degradome Analysis of the Bovine Piroplasmid Pathogens Babesia bovis and Theileria annulata |
| title_sort | comparative degradome analysis of the bovine piroplasmid pathogens babesia bovis and theileria annulata |
| topic | Babesia bovis Theileria annulata Enfermedades de los Animales Organismos Patógenos Ganado Bovino Animal Diseases Pathogens Cattle |
| url | http://hdl.handle.net/20.500.12123/14467 https://www.mdpi.com/2076-0817/12/2/237 https://doi.org/10.3390/pathogens12020237 |
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