Eprinomectin accumulation in Rhipicephalus (Boophilus) microplus : pharmacokinetic and efficacy assessment
Eprinomectin (EPM) is a macrocyclic lactone used against endo-ectoparasites without withdrawal time in milk and meat after its pour-on administration at 0.5 mg/kg. Previous experiments evaluated the efficacy of EPM against Rhipicephalus (Boophilus) microplus in cattle. This study assessed EPM effica...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Artículo |
| Language: | Inglés |
| Published: |
2017
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/1446 http://www.sciencedirect.com/science/article/pii/S0304401715300728 https://doi.org/10.1016/j.vetpar.2015.11.005 |
| _version_ | 1855482889324462080 |
|---|---|
| author | Lifschitz, Adrian Luis Nava, Santiago Mangold, Atilio Jose Imperiale, Fernanda Andrea Ballent, Mariana Canevari, Jose Tobias Lanusse, Carlos Edmundo |
| author_browse | Ballent, Mariana Canevari, Jose Tobias Imperiale, Fernanda Andrea Lanusse, Carlos Edmundo Lifschitz, Adrian Luis Mangold, Atilio Jose Nava, Santiago |
| author_facet | Lifschitz, Adrian Luis Nava, Santiago Mangold, Atilio Jose Imperiale, Fernanda Andrea Ballent, Mariana Canevari, Jose Tobias Lanusse, Carlos Edmundo |
| author_sort | Lifschitz, Adrian Luis |
| collection | INTA Digital |
| description | Eprinomectin (EPM) is a macrocyclic lactone used against endo-ectoparasites without withdrawal time in milk and meat after its pour-on administration at 0.5 mg/kg. Previous experiments evaluated the efficacy of EPM against Rhipicephalus (Boophilus) microplus in cattle. This study assessed EPM efficacy against R. (B.) microplus after topical administration at two dose rates and investigated the relationship between EPM systemic exposure in the host and drug concentrations accumulated in ticks recovered from treated animals. A standardized pharmaco-parasitological study was performed in two phases. In phase 1 eighteen Braford cattle naturally infected with R. (B.) microplus were divided into three experimental groups with a similar level of infestation (Kruskal–Wallis test, P > 0.05): control group and treated groups with EPM pour-on (1 and 1.5 mg/kg). Samples of heparinized blood and ticks at different life stages were taken between 0 and 21 days (d) post-administration to measure EPM concentrations by HPLC. The efficacy trial (phase 2) included eighteen Braford calves naturally infected with R. (B.) microplus divided into control group and 1 mg/kg and 1.5 mg/kg EPM treated groups. Female ticks (4.5–8 mm) on cattle were counted between 1 and 23 days post-treatment to evaluate the efficacy of EPM. The reproductive efficiency index (REI) and the fertility efficiency index (FEI) were evaluated. Plasma concentrations of EPM showed a linear relationship with the level of dose rate administered. Peak plasma concentrations were within a range between 13.8 and 90 ng/ml, which guarantee milk drug concentrations below the maximum residues level. High EPM concentrations were detected in ticks. EPM concentrations in R. (B.) microplus were correlated to plasma concentrations between 1.25 days and 21 days post-administration (r 0.84; P < 0.05). EPM efficacy calculated using the Henderson–Tilton formula was 98.9% and 99.1% (7 days post-administration) and 100% (23 days post-administration) after EPM treatment at 1 and 1.5 mg/kg, respectively. EPM administered at 1.5 mg/kg also showed a significantly higher deleterious effect on tick fertility as measured by FEI (P < 0.01). Therefore, treatment with EPM may be useful for controlling ticks in cattle, particularly in dairy production systems. |
| format | Artículo |
| id | INTA1446 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA14462019-03-25T15:17:55Z Eprinomectin accumulation in Rhipicephalus (Boophilus) microplus : pharmacokinetic and efficacy assessment Lifschitz, Adrian Luis Nava, Santiago Mangold, Atilio Jose Imperiale, Fernanda Andrea Ballent, Mariana Canevari, Jose Tobias Lanusse, Carlos Edmundo Rhipicephalus Ganado Bovino Cattle Pharmacokinetic Farmacocinética Rhipicephalus (Boophilus) Microplus Garrapatas Eprinomectina Eprinomectin (EPM) is a macrocyclic lactone used against endo-ectoparasites without withdrawal time in milk and meat after its pour-on administration at 0.5 mg/kg. Previous experiments evaluated the efficacy of EPM against Rhipicephalus (Boophilus) microplus in cattle. This study assessed EPM efficacy against R. (B.) microplus after topical administration at two dose rates and investigated the relationship between EPM systemic exposure in the host and drug concentrations accumulated in ticks recovered from treated animals. A standardized pharmaco-parasitological study was performed in two phases. In phase 1 eighteen Braford cattle naturally infected with R. (B.) microplus were divided into three experimental groups with a similar level of infestation (Kruskal–Wallis test, P > 0.05): control group and treated groups with EPM pour-on (1 and 1.5 mg/kg). Samples of heparinized blood and ticks at different life stages were taken between 0 and 21 days (d) post-administration to measure EPM concentrations by HPLC. The efficacy trial (phase 2) included eighteen Braford calves naturally infected with R. (B.) microplus divided into control group and 1 mg/kg and 1.5 mg/kg EPM treated groups. Female ticks (4.5–8 mm) on cattle were counted between 1 and 23 days post-treatment to evaluate the efficacy of EPM. The reproductive efficiency index (REI) and the fertility efficiency index (FEI) were evaluated. Plasma concentrations of EPM showed a linear relationship with the level of dose rate administered. Peak plasma concentrations were within a range between 13.8 and 90 ng/ml, which guarantee milk drug concentrations below the maximum residues level. High EPM concentrations were detected in ticks. EPM concentrations in R. (B.) microplus were correlated to plasma concentrations between 1.25 days and 21 days post-administration (r 0.84; P < 0.05). EPM efficacy calculated using the Henderson–Tilton formula was 98.9% and 99.1% (7 days post-administration) and 100% (23 days post-administration) after EPM treatment at 1 and 1.5 mg/kg, respectively. EPM administered at 1.5 mg/kg also showed a significantly higher deleterious effect on tick fertility as measured by FEI (P < 0.01). Therefore, treatment with EPM may be useful for controlling ticks in cattle, particularly in dairy production systems. Fil: Lifschitz, Adrian Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires.. Facultad de Ciencias Veterinarias; Argentina Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Mangold, Atilio Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Imperiale, F. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires.. Facultad de Ciencias Veterinarias; Argentina Fil: Ballent, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET- Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina Fil: Canevari, Jose Tobias. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Lanusse, Carlos Edmundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigación Veterinaria de Tandil; Argentina 2017-10-10T13:01:05Z 2017-10-10T13:01:05Z 2016-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/1446 http://www.sciencedirect.com/science/article/pii/S0304401715300728 0304-4017 https://doi.org/10.1016/j.vetpar.2015.11.005 eng info:eu-repo/semantics/restrictedAccess application/pdf Veterinary parasitology 215 : 11-16. (January 2016) |
| spellingShingle | Rhipicephalus Ganado Bovino Cattle Pharmacokinetic Farmacocinética Rhipicephalus (Boophilus) Microplus Garrapatas Eprinomectina Lifschitz, Adrian Luis Nava, Santiago Mangold, Atilio Jose Imperiale, Fernanda Andrea Ballent, Mariana Canevari, Jose Tobias Lanusse, Carlos Edmundo Eprinomectin accumulation in Rhipicephalus (Boophilus) microplus : pharmacokinetic and efficacy assessment |
| title | Eprinomectin accumulation in Rhipicephalus (Boophilus) microplus : pharmacokinetic and efficacy assessment |
| title_full | Eprinomectin accumulation in Rhipicephalus (Boophilus) microplus : pharmacokinetic and efficacy assessment |
| title_fullStr | Eprinomectin accumulation in Rhipicephalus (Boophilus) microplus : pharmacokinetic and efficacy assessment |
| title_full_unstemmed | Eprinomectin accumulation in Rhipicephalus (Boophilus) microplus : pharmacokinetic and efficacy assessment |
| title_short | Eprinomectin accumulation in Rhipicephalus (Boophilus) microplus : pharmacokinetic and efficacy assessment |
| title_sort | eprinomectin accumulation in rhipicephalus boophilus microplus pharmacokinetic and efficacy assessment |
| topic | Rhipicephalus Ganado Bovino Cattle Pharmacokinetic Farmacocinética Rhipicephalus (Boophilus) Microplus Garrapatas Eprinomectina |
| url | http://hdl.handle.net/20.500.12123/1446 http://www.sciencedirect.com/science/article/pii/S0304401715300728 https://doi.org/10.1016/j.vetpar.2015.11.005 |
| work_keys_str_mv | AT lifschitzadrianluis eprinomectinaccumulationinrhipicephalusboophilusmicropluspharmacokineticandefficacyassessment AT navasantiago eprinomectinaccumulationinrhipicephalusboophilusmicropluspharmacokineticandefficacyassessment AT mangoldatiliojose eprinomectinaccumulationinrhipicephalusboophilusmicropluspharmacokineticandefficacyassessment AT imperialefernandaandrea eprinomectinaccumulationinrhipicephalusboophilusmicropluspharmacokineticandefficacyassessment AT ballentmariana eprinomectinaccumulationinrhipicephalusboophilusmicropluspharmacokineticandefficacyassessment AT canevarijosetobias eprinomectinaccumulationinrhipicephalusboophilusmicropluspharmacokineticandefficacyassessment AT lanussecarlosedmundo eprinomectinaccumulationinrhipicephalusboophilusmicropluspharmacokineticandefficacyassessment |