Bovine leukemia virus encoded blv-miR-b4-3p microRNA is associated with reduced expression of anti-oncogenic gene in vivo
Bovine leukemia virus (BLV) is a retrovirus that causes malignant B-cell lymphoma in up to ten-percent of infected cattle. To date, the mechanisms of BLV linked to malignant transformation remain elusive. Although BLV-encoded miRNAs have been associated with the regulation of different genes involve...
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| Format: | Artículo |
| Language: | Inglés |
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PLOS
2023
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| Online Access: | http://hdl.handle.net/20.500.12123/14459 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0281317 https://doi.org/10.1371/journal.pone.0281317 |
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| author | Petersen, Marcos Iván Carignano, Hugo Adrian Mongini, Claudia Gonzalez, Diego D. Jaworski, Juan Pablo |
| author_browse | Carignano, Hugo Adrian Gonzalez, Diego D. Jaworski, Juan Pablo Mongini, Claudia Petersen, Marcos Iván |
| author_facet | Petersen, Marcos Iván Carignano, Hugo Adrian Mongini, Claudia Gonzalez, Diego D. Jaworski, Juan Pablo |
| author_sort | Petersen, Marcos Iván |
| collection | INTA Digital |
| description | Bovine leukemia virus (BLV) is a retrovirus that causes malignant B-cell lymphoma in up to ten-percent of infected cattle. To date, the mechanisms of BLV linked to malignant transformation remain elusive. Although BLV-encoded miRNAs have been associated with the regulation of different genes involved in oncogenic pathways, this association has not been evaluated in cattle naturally infected with BLV. The objective of this study was to determine the relative expression of BLV-encoded miRNA blv-miR-b4-3p, the host analogous miRNA bo-miR-29a and a couple of potential target mRNAs (HBP-1 and PXDN, with anti-tumorigenic function in B-cells), in cattle naturally infected with BLV compared to uninfected animals (control group). We observed that PXDN was significantly downregulated in BLV-infected cattle (P = 0.03). Considering the similar expression of endogenous bo-miR-29a in both animal groups, the downregulation of PXDN in BLV-naturally infected cattle could be linked to blv-miR-b4-3p expression in these animals. Knowing that PXDN is involved in anti-tumoral pathways in B-cells, the results presented here suggest that blv-miR-b4-3p might be involved in BLV tumorigenesis during natural infection with BLV in cattle. |
| format | Artículo |
| id | INTA14459 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | PLOS |
| publisherStr | PLOS |
| record_format | dspace |
| spelling | INTA144592023-04-12T17:31:47Z Bovine leukemia virus encoded blv-miR-b4-3p microRNA is associated with reduced expression of anti-oncogenic gene in vivo Petersen, Marcos Iván Carignano, Hugo Adrian Mongini, Claudia Gonzalez, Diego D. Jaworski, Juan Pablo Ganado Bovino Enfermedades de los Animales Virus Leucemia Bovina MicroARN Oncogenicidad Cattle Animal Diseases Bovine Leukemia Virus MicroRNA Oncogenicity Bovine leukemia virus (BLV) is a retrovirus that causes malignant B-cell lymphoma in up to ten-percent of infected cattle. To date, the mechanisms of BLV linked to malignant transformation remain elusive. Although BLV-encoded miRNAs have been associated with the regulation of different genes involved in oncogenic pathways, this association has not been evaluated in cattle naturally infected with BLV. The objective of this study was to determine the relative expression of BLV-encoded miRNA blv-miR-b4-3p, the host analogous miRNA bo-miR-29a and a couple of potential target mRNAs (HBP-1 and PXDN, with anti-tumorigenic function in B-cells), in cattle naturally infected with BLV compared to uninfected animals (control group). We observed that PXDN was significantly downregulated in BLV-infected cattle (P = 0.03). Considering the similar expression of endogenous bo-miR-29a in both animal groups, the downregulation of PXDN in BLV-naturally infected cattle could be linked to blv-miR-b4-3p expression in these animals. Knowing that PXDN is involved in anti-tumoral pathways in B-cells, the results presented here suggest that blv-miR-b4-3p might be involved in BLV tumorigenesis during natural infection with BLV in cattle. Instituto de Virología Fil: Petersen, Marcos Iván. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Petersen, Marcos Iván. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Carignano, Hugo Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Carignano, Hugo Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Mongini, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Mongini, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gonzalez, Diego D..Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Gonzalez, Diego D. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Jaworski, Juan Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Jaworski, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2023-04-12T17:29:11Z 2023-04-12T17:29:11Z 2023-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/14459 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0281317 1932-6203 https://doi.org/10.1371/journal.pone.0281317 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf PLOS PLoS ONE 18 (2) : e0281317. (February 2023) |
| spellingShingle | Ganado Bovino Enfermedades de los Animales Virus Leucemia Bovina MicroARN Oncogenicidad Cattle Animal Diseases Bovine Leukemia Virus MicroRNA Oncogenicity Petersen, Marcos Iván Carignano, Hugo Adrian Mongini, Claudia Gonzalez, Diego D. Jaworski, Juan Pablo Bovine leukemia virus encoded blv-miR-b4-3p microRNA is associated with reduced expression of anti-oncogenic gene in vivo |
| title | Bovine leukemia virus encoded blv-miR-b4-3p microRNA is associated with reduced expression of anti-oncogenic gene in vivo |
| title_full | Bovine leukemia virus encoded blv-miR-b4-3p microRNA is associated with reduced expression of anti-oncogenic gene in vivo |
| title_fullStr | Bovine leukemia virus encoded blv-miR-b4-3p microRNA is associated with reduced expression of anti-oncogenic gene in vivo |
| title_full_unstemmed | Bovine leukemia virus encoded blv-miR-b4-3p microRNA is associated with reduced expression of anti-oncogenic gene in vivo |
| title_short | Bovine leukemia virus encoded blv-miR-b4-3p microRNA is associated with reduced expression of anti-oncogenic gene in vivo |
| title_sort | bovine leukemia virus encoded blv mir b4 3p microrna is associated with reduced expression of anti oncogenic gene in vivo |
| topic | Ganado Bovino Enfermedades de los Animales Virus Leucemia Bovina MicroARN Oncogenicidad Cattle Animal Diseases Bovine Leukemia Virus MicroRNA Oncogenicity |
| url | http://hdl.handle.net/20.500.12123/14459 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0281317 https://doi.org/10.1371/journal.pone.0281317 |
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