Transferability of microsatellite markers located in candidate genes for wood properties between Eucalyptus species
AIM OF STUDY: To analyze the feasibility of extrapolating conclusions on wood quality genetic control between different Eucalyptus species, particularly from species with better genomic information, to those less characterized. For this purpose, the first step is to analyze the conservation and cros...
| Autores principales: | , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (España)
2023
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/14432 https://revistas.inia.es/index.php/fs/article/view/5279 https://doi.org/10.5424/fs/2014233-05279 |
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| author | Acuña, Cintia Vanesa Villalba, Pamela Victoria Hopp, Horacio Esteban Marcucci Poltri, Susana Noemi |
| author_browse | Acuña, Cintia Vanesa Hopp, Horacio Esteban Marcucci Poltri, Susana Noemi Villalba, Pamela Victoria |
| author_facet | Acuña, Cintia Vanesa Villalba, Pamela Victoria Hopp, Horacio Esteban Marcucci Poltri, Susana Noemi |
| author_sort | Acuña, Cintia Vanesa |
| collection | INTA Digital |
| description | AIM OF STUDY: To analyze the feasibility of extrapolating conclusions on wood quality genetic control between different Eucalyptus species, particularly from species with better genomic information, to those less characterized. For this purpose, the first step is to analyze the conservation and cross-transferability of microsatellites markers (SSRs) located in candidate genes. AREA OF STUDY: Eucalyptus species implanted in Argentina coming from different Australian origins. MATERIALS AND METHODS: Twelve validated and polymorphic SSRs in candidate genes (SSR-CGs) for wood quality in E. globulus were selected for cross species amplification in six species: E. grandis, E. saligna, E. dunnii, E. viminalis, E. camaldulensis and E. tereticornis. MAIN RESULTS: High cross-species transferability (92% to 100%) was found for the 12 polymorphic SSRs detected in E. globulus. These markers revealed allelic diversity in nine important candidate genes: cinnamoyl CoA reductase (CCR), cellulose synthase 3 (CesA3), the transcription factor LIM1, homocysteine S-methyltransferase (HMT), shikimate kinase (SK), xyloglucan endotransglycosylase 2 (XTH2), glutathione S-transferase (GST), glutamate decarboxylase (GAD) and peroxidase (PER). RESEARCH HIGHLIGHTS: The markers described are potentially suitable for comparative QTL mapping, molecular marker assisted breeding (MAB) and for population genetic studies across different species within the subgenus Symphyomyrtus. |
| format | info:ar-repo/semantics/artículo |
| id | INTA14432 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (España) |
| publisherStr | Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (España) |
| record_format | dspace |
| spelling | INTA144322023-04-10T15:03:06Z Transferability of microsatellite markers located in candidate genes for wood properties between Eucalyptus species Acuña, Cintia Vanesa Villalba, Pamela Victoria Hopp, Horacio Esteban Marcucci Poltri, Susana Noemi Eucalyptus Microsatélites Marcadores Genéticos Variedades Cruzamiento Microsatellites Genetic Markers Varieties Cross-breeding AIM OF STUDY: To analyze the feasibility of extrapolating conclusions on wood quality genetic control between different Eucalyptus species, particularly from species with better genomic information, to those less characterized. For this purpose, the first step is to analyze the conservation and cross-transferability of microsatellites markers (SSRs) located in candidate genes. AREA OF STUDY: Eucalyptus species implanted in Argentina coming from different Australian origins. MATERIALS AND METHODS: Twelve validated and polymorphic SSRs in candidate genes (SSR-CGs) for wood quality in E. globulus were selected for cross species amplification in six species: E. grandis, E. saligna, E. dunnii, E. viminalis, E. camaldulensis and E. tereticornis. MAIN RESULTS: High cross-species transferability (92% to 100%) was found for the 12 polymorphic SSRs detected in E. globulus. These markers revealed allelic diversity in nine important candidate genes: cinnamoyl CoA reductase (CCR), cellulose synthase 3 (CesA3), the transcription factor LIM1, homocysteine S-methyltransferase (HMT), shikimate kinase (SK), xyloglucan endotransglycosylase 2 (XTH2), glutathione S-transferase (GST), glutamate decarboxylase (GAD) and peroxidase (PER). RESEARCH HIGHLIGHTS: The markers described are potentially suitable for comparative QTL mapping, molecular marker assisted breeding (MAB) and for population genetic studies across different species within the subgenus Symphyomyrtus. Instituto de Biotecnología Fil: Acuña, Cintia Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Villalba, Pamela Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Hopp, Horacio Esteban. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Marcucci Poltri, Susana Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina 2023-04-10T15:00:30Z 2023-04-10T15:00:30Z 2014 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/14432 https://revistas.inia.es/index.php/fs/article/view/5279 2171-5068 2171-9845 https://doi.org/10.5424/fs/2014233-05279 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (España) Forest Systems 23 (3) : 506-512. (2014) |
| spellingShingle | Eucalyptus Microsatélites Marcadores Genéticos Variedades Cruzamiento Microsatellites Genetic Markers Varieties Cross-breeding Acuña, Cintia Vanesa Villalba, Pamela Victoria Hopp, Horacio Esteban Marcucci Poltri, Susana Noemi Transferability of microsatellite markers located in candidate genes for wood properties between Eucalyptus species |
| title | Transferability of microsatellite markers located in candidate genes for wood properties between Eucalyptus species |
| title_full | Transferability of microsatellite markers located in candidate genes for wood properties between Eucalyptus species |
| title_fullStr | Transferability of microsatellite markers located in candidate genes for wood properties between Eucalyptus species |
| title_full_unstemmed | Transferability of microsatellite markers located in candidate genes for wood properties between Eucalyptus species |
| title_short | Transferability of microsatellite markers located in candidate genes for wood properties between Eucalyptus species |
| title_sort | transferability of microsatellite markers located in candidate genes for wood properties between eucalyptus species |
| topic | Eucalyptus Microsatélites Marcadores Genéticos Variedades Cruzamiento Microsatellites Genetic Markers Varieties Cross-breeding |
| url | http://hdl.handle.net/20.500.12123/14432 https://revistas.inia.es/index.php/fs/article/view/5279 https://doi.org/10.5424/fs/2014233-05279 |
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