An applied printing immunoassay with recombinant Nc-SAG1 for detection of antibodies to Neospora caninum in cattle
Neospora caninum is a protozoan parasite that causes an important reproductive disease in cattle. Neospora caninum surface antigen 1 (Nc-SAG1) is an immunodominant candidate for the development of a diagnostic reagent for neosporosis. The current study describes the development and evaluation of an...
| Autores principales: | , , , , , , , |
|---|---|
| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Sage Publishing
2023
|
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/14429 https://journals.sagepub.com/doi/10.1177/1040638711416845 https://doi.org/10.1177/1040638711416845 |
| _version_ | 1855485360353574912 |
|---|---|
| author | Wilkowsky, Silvina Elizabeth Gimenez Bareiro, Guillermo Mon, Maria Laura Moore, Prando Dadin Caspe, Sergio Gaston Campero, Carlos Manuel Fort, Marcelo Cristian Romano, Maria Isabel |
| author_browse | Campero, Carlos Manuel Caspe, Sergio Gaston Fort, Marcelo Cristian Gimenez Bareiro, Guillermo Mon, Maria Laura Moore, Prando Dadin Romano, Maria Isabel Wilkowsky, Silvina Elizabeth |
| author_facet | Wilkowsky, Silvina Elizabeth Gimenez Bareiro, Guillermo Mon, Maria Laura Moore, Prando Dadin Caspe, Sergio Gaston Campero, Carlos Manuel Fort, Marcelo Cristian Romano, Maria Isabel |
| author_sort | Wilkowsky, Silvina Elizabeth |
| collection | INTA Digital |
| description | Neospora caninum is a protozoan parasite that causes an important reproductive disease in cattle. Neospora caninum surface antigen 1 (Nc-SAG1) is an immunodominant candidate for the development of a diagnostic reagent for neosporosis. The current study describes the development and evaluation of an antigen print immunoassay (APIA) with recombinant Nc-SAG1 for the detection of specific antibodies to N. caninum in cattle. The concordance between APIA and a commercial enzyme-linked immunosorbent assay (ELISA) was evaluated with 232 serum samples from experimentally and naturally infected cattle. Sixty-one (26.7%) samples were positive for antibodies to N. caninum by ELISA and 58 (25.4%) by APIA. The new assay had a sensitivity of 85% and a specificity of 96%. These results, along with the potential of APIA to evolve into a multiple antigen detection format, suggest that this method would be a reliable diagnostic test for detection of antibodies to N. caninum in cattle. |
| format | Artículo |
| id | INTA14429 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Sage Publishing |
| publisherStr | Sage Publishing |
| record_format | dspace |
| spelling | INTA144292023-04-10T13:45:30Z An applied printing immunoassay with recombinant Nc-SAG1 for detection of antibodies to Neospora caninum in cattle Wilkowsky, Silvina Elizabeth Gimenez Bareiro, Guillermo Mon, Maria Laura Moore, Prando Dadin Caspe, Sergio Gaston Campero, Carlos Manuel Fort, Marcelo Cristian Romano, Maria Isabel Ganado Bovino Anticuerpos Inmunoensayos Diagnóstico Cattle Neospora caninum Antibodies Immunoassays Diagnosis Neospora caninum is a protozoan parasite that causes an important reproductive disease in cattle. Neospora caninum surface antigen 1 (Nc-SAG1) is an immunodominant candidate for the development of a diagnostic reagent for neosporosis. The current study describes the development and evaluation of an antigen print immunoassay (APIA) with recombinant Nc-SAG1 for the detection of specific antibodies to N. caninum in cattle. The concordance between APIA and a commercial enzyme-linked immunosorbent assay (ELISA) was evaluated with 232 serum samples from experimentally and naturally infected cattle. Sixty-one (26.7%) samples were positive for antibodies to N. caninum by ELISA and 58 (25.4%) by APIA. The new assay had a sensitivity of 85% and a specificity of 96%. These results, along with the potential of APIA to evolve into a multiple antigen detection format, suggest that this method would be a reliable diagnostic test for detection of antibodies to N. caninum in cattle. Instituto de Biotecnología Fil: Wilkowsky, Silvina Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Fil: Gimenez Bareiro, Guillermo. Universidad Nacional de Asunción. Faculty of Veterinary Science; Paraguay Fil: Mon, Maria Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Fil: Moore, Prando Dadin. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Caspe, Sergio Gaston. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina Fil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Fort, Marcelo Cristian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; Argentina Fil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. 2023-04-10T13:43:31Z 2023-04-10T13:43:31Z 2011-08 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/14429 https://journals.sagepub.com/doi/10.1177/1040638711416845 1040-6387 1943-4936 https://doi.org/10.1177/1040638711416845 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Sage Publishing Journal of Veterinary Diagnostic Investigation 23 (5) : 971-976. (August 2011) |
| spellingShingle | Ganado Bovino Anticuerpos Inmunoensayos Diagnóstico Cattle Neospora caninum Antibodies Immunoassays Diagnosis Wilkowsky, Silvina Elizabeth Gimenez Bareiro, Guillermo Mon, Maria Laura Moore, Prando Dadin Caspe, Sergio Gaston Campero, Carlos Manuel Fort, Marcelo Cristian Romano, Maria Isabel An applied printing immunoassay with recombinant Nc-SAG1 for detection of antibodies to Neospora caninum in cattle |
| title | An applied printing immunoassay with recombinant Nc-SAG1 for detection of antibodies to Neospora caninum in cattle |
| title_full | An applied printing immunoassay with recombinant Nc-SAG1 for detection of antibodies to Neospora caninum in cattle |
| title_fullStr | An applied printing immunoassay with recombinant Nc-SAG1 for detection of antibodies to Neospora caninum in cattle |
| title_full_unstemmed | An applied printing immunoassay with recombinant Nc-SAG1 for detection of antibodies to Neospora caninum in cattle |
| title_short | An applied printing immunoassay with recombinant Nc-SAG1 for detection of antibodies to Neospora caninum in cattle |
| title_sort | applied printing immunoassay with recombinant nc sag1 for detection of antibodies to neospora caninum in cattle |
| topic | Ganado Bovino Anticuerpos Inmunoensayos Diagnóstico Cattle Neospora caninum Antibodies Immunoassays Diagnosis |
| url | http://hdl.handle.net/20.500.12123/14429 https://journals.sagepub.com/doi/10.1177/1040638711416845 https://doi.org/10.1177/1040638711416845 |
| work_keys_str_mv | AT wilkowskysilvinaelizabeth anappliedprintingimmunoassaywithrecombinantncsag1fordetectionofantibodiestoneosporacaninumincattle AT gimenezbareiroguillermo anappliedprintingimmunoassaywithrecombinantncsag1fordetectionofantibodiestoneosporacaninumincattle AT monmarialaura anappliedprintingimmunoassaywithrecombinantncsag1fordetectionofantibodiestoneosporacaninumincattle AT mooreprandodadin anappliedprintingimmunoassaywithrecombinantncsag1fordetectionofantibodiestoneosporacaninumincattle AT caspesergiogaston anappliedprintingimmunoassaywithrecombinantncsag1fordetectionofantibodiestoneosporacaninumincattle AT camperocarlosmanuel anappliedprintingimmunoassaywithrecombinantncsag1fordetectionofantibodiestoneosporacaninumincattle AT fortmarcelocristian anappliedprintingimmunoassaywithrecombinantncsag1fordetectionofantibodiestoneosporacaninumincattle AT romanomariaisabel anappliedprintingimmunoassaywithrecombinantncsag1fordetectionofantibodiestoneosporacaninumincattle AT wilkowskysilvinaelizabeth appliedprintingimmunoassaywithrecombinantncsag1fordetectionofantibodiestoneosporacaninumincattle AT gimenezbareiroguillermo appliedprintingimmunoassaywithrecombinantncsag1fordetectionofantibodiestoneosporacaninumincattle AT monmarialaura appliedprintingimmunoassaywithrecombinantncsag1fordetectionofantibodiestoneosporacaninumincattle AT mooreprandodadin appliedprintingimmunoassaywithrecombinantncsag1fordetectionofantibodiestoneosporacaninumincattle AT caspesergiogaston appliedprintingimmunoassaywithrecombinantncsag1fordetectionofantibodiestoneosporacaninumincattle AT camperocarlosmanuel appliedprintingimmunoassaywithrecombinantncsag1fordetectionofantibodiestoneosporacaninumincattle AT fortmarcelocristian appliedprintingimmunoassaywithrecombinantncsag1fordetectionofantibodiestoneosporacaninumincattle AT romanomariaisabel appliedprintingimmunoassaywithrecombinantncsag1fordetectionofantibodiestoneosporacaninumincattle |