Realistic scenarios of pesticide exposure alters multiple biomarkers in BOANA PULCHELLA (ANURA) Adult Frogs

Imazethapyr, a post-emergent herbicide used in worldwide soybean and corn crops, induces genetic and biochemical alterations in aquatic vertebrates. This study examined the relationship between biomarkers at different organization levels and imazethapyr real-life route exposure in Boana pulchella ad...

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Main Authors: Pérez-Iglesias, Juan Manuel, Natale, Guillermo S., Brodeur, Celine Marie, Larramendy, Marcelo L.
Format: Artículo
Language:Inglés
Published: Springer 2023
Subjects:
Online Access:http://hdl.handle.net/20.500.12123/14340
https://link.springer.com/article/10.1007/s10646-023-02639-6
https://doi.org/10.1007/s10646-023-02639-6
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author Pérez-Iglesias, Juan Manuel
Natale, Guillermo S.
Brodeur, Celine Marie
Larramendy, Marcelo L.
author_browse Brodeur, Celine Marie
Larramendy, Marcelo L.
Natale, Guillermo S.
Pérez-Iglesias, Juan Manuel
author_facet Pérez-Iglesias, Juan Manuel
Natale, Guillermo S.
Brodeur, Celine Marie
Larramendy, Marcelo L.
author_sort Pérez-Iglesias, Juan Manuel
collection INTA Digital
description Imazethapyr, a post-emergent herbicide used in worldwide soybean and corn crops, induces genetic and biochemical alterations in aquatic vertebrates. This study examined the relationship between biomarkers at different organization levels and imazethapyr real-life route exposure in Boana pulchella adults. Frogs were exposed to imazethapyr-based formulation Pivot® H (10.59%) at concentrations representing possible acute routes: field runoff (S1:10 mg.L−1), exposure after direct foliar application (S2:100 mg.L−1) and during direct foliar application (S3:1000 mg.L−1). Post-exposure, endpoints levels were evaluated: organism alterations, biochemical activities and cytogenetic assays. Forty-eight hours post-exposure, antioxidant enzymes decrease, micronuclei induction and DNA damage were observed in all scenarios, while cholinesterase activity increase and body condition reduction were observed in frog-exposed to S3. Ninety-six hours postexposure, frogs showed glutathione-S-transferase inhibition in S1, micronuclei induction in S2 and S3, and DNA-damage increase in S3. Herbicides routes of exposures in real-life could indicate that authorized applications have a risk to amphibian populations.
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spelling INTA143402023-03-28T18:09:06Z Realistic scenarios of pesticide exposure alters multiple biomarkers in BOANA PULCHELLA (ANURA) Adult Frogs Pérez-Iglesias, Juan Manuel Natale, Guillermo S. Brodeur, Celine Marie Larramendy, Marcelo L. Rana Fitotoxicidad Phytotoxicity Frogs Pesticides Plaguicidas Biomarkers Integrative Responses Dermal Exposure Imazethapyr Phisiological Stress Respuestas Integradoras de Biomarcadores Exposición Dérmica Estrés Fisiológico Boana pulchella Imazethapyr, a post-emergent herbicide used in worldwide soybean and corn crops, induces genetic and biochemical alterations in aquatic vertebrates. This study examined the relationship between biomarkers at different organization levels and imazethapyr real-life route exposure in Boana pulchella adults. Frogs were exposed to imazethapyr-based formulation Pivot® H (10.59%) at concentrations representing possible acute routes: field runoff (S1:10 mg.L−1), exposure after direct foliar application (S2:100 mg.L−1) and during direct foliar application (S3:1000 mg.L−1). Post-exposure, endpoints levels were evaluated: organism alterations, biochemical activities and cytogenetic assays. Forty-eight hours post-exposure, antioxidant enzymes decrease, micronuclei induction and DNA damage were observed in all scenarios, while cholinesterase activity increase and body condition reduction were observed in frog-exposed to S3. Ninety-six hours postexposure, frogs showed glutathione-S-transferase inhibition in S1, micronuclei induction in S2 and S3, and DNA-damage increase in S3. Herbicides routes of exposures in real-life could indicate that authorized applications have a risk to amphibian populations. Instituto de Recursos Biológicos Fil: Pérez Iglesias, J.M. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigaciones del Medio Ambiente (CIM); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina. Fil: Natale, G.S. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigaciones del Medio Ambiente (CIM); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Brodeur, Celine Marie Julie. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Larramendy, Marcelo L. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2023-03-28T17:29:38Z 2023-03-28T17:29:38Z 2023-03-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/14340 https://link.springer.com/article/10.1007/s10646-023-02639-6 1573-3017 0963-9292 https://doi.org/10.1007/s10646-023-02639-6 eng info:eu-repograntAgreement/INTA/2019-PE-E2-I054-001/2019-PE-E2-I054-001/AR./Gestión sostenible de fitosanitarios info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Ecotoxicology 32 (2) : 1-12 (Published: 16 March 2023)
spellingShingle Rana
Fitotoxicidad
Phytotoxicity
Frogs
Pesticides
Plaguicidas
Biomarkers Integrative Responses
Dermal Exposure
Imazethapyr
Phisiological Stress
Respuestas Integradoras de Biomarcadores
Exposición Dérmica
Estrés Fisiológico
Boana pulchella
Pérez-Iglesias, Juan Manuel
Natale, Guillermo S.
Brodeur, Celine Marie
Larramendy, Marcelo L.
Realistic scenarios of pesticide exposure alters multiple biomarkers in BOANA PULCHELLA (ANURA) Adult Frogs
title Realistic scenarios of pesticide exposure alters multiple biomarkers in BOANA PULCHELLA (ANURA) Adult Frogs
title_full Realistic scenarios of pesticide exposure alters multiple biomarkers in BOANA PULCHELLA (ANURA) Adult Frogs
title_fullStr Realistic scenarios of pesticide exposure alters multiple biomarkers in BOANA PULCHELLA (ANURA) Adult Frogs
title_full_unstemmed Realistic scenarios of pesticide exposure alters multiple biomarkers in BOANA PULCHELLA (ANURA) Adult Frogs
title_short Realistic scenarios of pesticide exposure alters multiple biomarkers in BOANA PULCHELLA (ANURA) Adult Frogs
title_sort realistic scenarios of pesticide exposure alters multiple biomarkers in boana pulchella anura adult frogs
topic Rana
Fitotoxicidad
Phytotoxicity
Frogs
Pesticides
Plaguicidas
Biomarkers Integrative Responses
Dermal Exposure
Imazethapyr
Phisiological Stress
Respuestas Integradoras de Biomarcadores
Exposición Dérmica
Estrés Fisiológico
Boana pulchella
url http://hdl.handle.net/20.500.12123/14340
https://link.springer.com/article/10.1007/s10646-023-02639-6
https://doi.org/10.1007/s10646-023-02639-6
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