Molecular evidence and additional morphological characters to distinguish Ornithodoros brodyi and Ornithodoros yumatensis (Ixodida: Argasidae) in their different developmental stages

Ornithodoros brodyi and Ornithodoros yumatensis are two species distributed in the Americas and associated with bats and caves. Both species have similar morphology, and the diagnostic traits of adults have not been detailed or illustrated accurately. In this study, the independence of both species...

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Autores principales: Guzman Cornejo, Carmen M., Garcia-Prieto, Luis, Rebollo-Hernandez, Andrea, Nava, Santiago, Sanches-Montes, Sokani, Venzal, José Manuel
Formato: Artículo
Lenguaje:Inglés
Publicado: 2017
Materias:
Acceso en línea:http://hdl.handle.net/20.500.12123/1426
https://www.degruyter.com/view/j/ap.2017.62.issue-2/ap-2017-0051/ap-2017-0051.xml
https://doi.org/10.1515/ap-2017-0051
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author Guzman Cornejo, Carmen M.
Garcia-Prieto, Luis
Rebollo-Hernandez, Andrea
Nava, Santiago
Sanches-Montes, Sokani
Venzal, José Manuel
author_browse Garcia-Prieto, Luis
Guzman Cornejo, Carmen M.
Nava, Santiago
Rebollo-Hernandez, Andrea
Sanches-Montes, Sokani
Venzal, José Manuel
author_facet Guzman Cornejo, Carmen M.
Garcia-Prieto, Luis
Rebollo-Hernandez, Andrea
Nava, Santiago
Sanches-Montes, Sokani
Venzal, José Manuel
author_sort Guzman Cornejo, Carmen M.
collection INTA Digital
description Ornithodoros brodyi and Ornithodoros yumatensis are two species distributed in the Americas and associated with bats and caves. Both species have similar morphology, and the diagnostic traits of adults have not been detailed or illustrated accurately. In this study, the independence of both species is validated on the basis of molecular evidence (using partial sequences of 16S rDNA gene), and the morphological differences between them (dentition of the hypostome and traits of individual mammillae) are confirmed through light and scanning electron microscopy. In addition to the above characteristics, we observed other traits that may serve to differentiate both species: dorsal setae are short and thick in O. yumatensis and are thin and moderate in size in O. brodyi. We also observed a conspicuous hood in O. brodyi, which was absent in O. yumatensis. Another characteristic observed is a line of setae, near the end of Tarsi II-IV, which in O. brodyi is formed by less than five setae and in O. yumatensis by more than five. The main morphological difference between larvae of the 2 species is the number of ventral setae [9 (4 circumanal pairs) in O. brodyi and 8 (3 circumanal pairs) in O. yumatensis]. The genetic divergence in 16S rDNA sequences between these two species ranges from 9.7 to 10.6%.
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spelling INTA14262019-12-26T15:23:48Z Molecular evidence and additional morphological characters to distinguish Ornithodoros brodyi and Ornithodoros yumatensis (Ixodida: Argasidae) in their different developmental stages Guzman Cornejo, Carmen M. Garcia-Prieto, Luis Rebollo-Hernandez, Andrea Nava, Santiago Sanches-Montes, Sokani Venzal, José Manuel Ornithodoros Metastigmata Morfología Morphology Garrapatas Ornithodoros Brodyi Ornithodoros Yumatensis Ornithodoros brodyi and Ornithodoros yumatensis are two species distributed in the Americas and associated with bats and caves. Both species have similar morphology, and the diagnostic traits of adults have not been detailed or illustrated accurately. In this study, the independence of both species is validated on the basis of molecular evidence (using partial sequences of 16S rDNA gene), and the morphological differences between them (dentition of the hypostome and traits of individual mammillae) are confirmed through light and scanning electron microscopy. In addition to the above characteristics, we observed other traits that may serve to differentiate both species: dorsal setae are short and thick in O. yumatensis and are thin and moderate in size in O. brodyi. We also observed a conspicuous hood in O. brodyi, which was absent in O. yumatensis. Another characteristic observed is a line of setae, near the end of Tarsi II-IV, which in O. brodyi is formed by less than five setae and in O. yumatensis by more than five. The main morphological difference between larvae of the 2 species is the number of ventral setae [9 (4 circumanal pairs) in O. brodyi and 8 (3 circumanal pairs) in O. yumatensis]. The genetic divergence in 16S rDNA sequences between these two species ranges from 9.7 to 10.6%. EEA Rafaela Fil: Guzman-Cornejo, Carmen. Universidad Nacional Autónoma de México. Facultad de Ciencias. Departamento de Biología Comparada. Laboratorio de Acarología; México Fil: Garcia-Prieto, Luis. Universidad Nacional Autónoma de México. Colección Nacional de Helmintos. Instituto de Biología; México Fil: Rebollo-Hernandez, Andrea. Universidad Nacional Autónoma de México. Facultad de Ciencias. Departamento de Biología Comparada. Laboratorio de Acarología; México Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Sanches-Montes, Sokani. Universidad Nacional Autónoma de México. Facultad de Ciencias. Departamento de Biología Comparada. Laboratorio de Acarología; México Fil: Venzal, José M. Universidad de la República. Facultad de Veterinaria. Laboratorio de Vectores y Enfermedades Transmitidas; Uruguay 2017-10-06T12:44:12Z 2017-10-06T12:44:12Z 2017 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/1426 https://www.degruyter.com/view/j/ap.2017.62.issue-2/ap-2017-0051/ap-2017-0051.xml 1230-2821 (Print) 1896-1851 (Online) https://doi.org/10.1515/ap-2017-0051 eng info:eu-repo/semantics/restrictedAccess application/pdf Acta parasitologica 62 (2) : 432-448. (2017)
spellingShingle Ornithodoros
Metastigmata
Morfología
Morphology
Garrapatas
Ornithodoros Brodyi
Ornithodoros Yumatensis
Guzman Cornejo, Carmen M.
Garcia-Prieto, Luis
Rebollo-Hernandez, Andrea
Nava, Santiago
Sanches-Montes, Sokani
Venzal, José Manuel
Molecular evidence and additional morphological characters to distinguish Ornithodoros brodyi and Ornithodoros yumatensis (Ixodida: Argasidae) in their different developmental stages
title Molecular evidence and additional morphological characters to distinguish Ornithodoros brodyi and Ornithodoros yumatensis (Ixodida: Argasidae) in their different developmental stages
title_full Molecular evidence and additional morphological characters to distinguish Ornithodoros brodyi and Ornithodoros yumatensis (Ixodida: Argasidae) in their different developmental stages
title_fullStr Molecular evidence and additional morphological characters to distinguish Ornithodoros brodyi and Ornithodoros yumatensis (Ixodida: Argasidae) in their different developmental stages
title_full_unstemmed Molecular evidence and additional morphological characters to distinguish Ornithodoros brodyi and Ornithodoros yumatensis (Ixodida: Argasidae) in their different developmental stages
title_short Molecular evidence and additional morphological characters to distinguish Ornithodoros brodyi and Ornithodoros yumatensis (Ixodida: Argasidae) in their different developmental stages
title_sort molecular evidence and additional morphological characters to distinguish ornithodoros brodyi and ornithodoros yumatensis ixodida argasidae in their different developmental stages
topic Ornithodoros
Metastigmata
Morfología
Morphology
Garrapatas
Ornithodoros Brodyi
Ornithodoros Yumatensis
url http://hdl.handle.net/20.500.12123/1426
https://www.degruyter.com/view/j/ap.2017.62.issue-2/ap-2017-0051/ap-2017-0051.xml
https://doi.org/10.1515/ap-2017-0051
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