Biodiversity of Aspergillus section Nigri populations in Argentinian vineyards and ochratoxin A contamination

Aspergillus section Nigri are described as the main source of ochratoxin A (OTA) contamination in grapes and wine worldwide. The knowledge of the factors affecting grape contamination by species included in this section and OTA production is essential to be able to reduce their presence, not only to...

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Main Authors: Chiotta, María Laura, Ponsone, Marí­a Lorena, Sosa, Débora María, Combina, Mariana, Chulze, Sofia Noemí
Format: info:ar-repo/semantics/artículo
Language:Inglés
Published: 2017
Subjects:
Online Access:http://hdl.handle.net/20.500.12123/1424
http://www.sciencedirect.com/science/article/pii/S0740002013000725
https://doi.org/10.1016/j.fm.2013.04.003
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author Chiotta, María Laura
Ponsone, Marí­a Lorena
Sosa, Débora María
Combina, Mariana
Chulze, Sofia Noemí
author_browse Chiotta, María Laura
Chulze, Sofia Noemí
Combina, Mariana
Ponsone, Marí­a Lorena
Sosa, Débora María
author_facet Chiotta, María Laura
Ponsone, Marí­a Lorena
Sosa, Débora María
Combina, Mariana
Chulze, Sofia Noemí
author_sort Chiotta, María Laura
collection INTA Digital
description Aspergillus section Nigri are described as the main source of ochratoxin A (OTA) contamination in grapes and wine worldwide. The knowledge of the factors affecting grape contamination by species included in this section and OTA production is essential to be able to reduce their presence, not only to improve wine quality, but also to maintain their safety. Therefore, the aims of this study were to determine the incidence of Aspergillus section Nigri species harvested in different grape-growing regions from Argentina, their ability to produce OTA, to correlate with meteorological conditions and geographical coordinates with their prevalence and to evaluate the OTA natural occurrence in grapes and wines. The morphological identification showed that Aspergillus niger aggregate species were the most prevalent ones, followed by Aspergillus carbonarius and Aspergillus uniseriate. These populations were confirmed through using AFLP markers and sequencing and, Aspergillus tubingensis was separated from A. niger aggregate. Climatic factors, altitude, longitude and latitude have influenced on the distribution of species included in the section. A. carbonarius and A. niger were OTA producers but differed in their OTA producing ability. Temperature was the factor which influenced the most over the highest incidence of A. carbonarius in La Rioja and San Juan regions. The trellis system in vineyards and drip irrigation also influenced the species isolation. The OTA levels detected in grapes and wines were low, but grape variety was more important in susceptibility to fungal infection and OTA levels.
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institution Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina)
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spelling INTA14242019-06-05T11:17:11Z Biodiversity of Aspergillus section Nigri populations in Argentinian vineyards and ochratoxin A contamination Chiotta, María Laura Ponsone, Marí­a Lorena Sosa, Débora María Combina, Mariana Chulze, Sofia Noemí Vid Grapevines Wines Mycotoxins Identification Ochratoxins Vinos Micotoxinas Aspergillus Identificación Ocratoxinas Argentina Ochratoxin A Agro-ecological Conditions Aspergillus Section Nigri Aspergillus section Nigri are described as the main source of ochratoxin A (OTA) contamination in grapes and wine worldwide. The knowledge of the factors affecting grape contamination by species included in this section and OTA production is essential to be able to reduce their presence, not only to improve wine quality, but also to maintain their safety. Therefore, the aims of this study were to determine the incidence of Aspergillus section Nigri species harvested in different grape-growing regions from Argentina, their ability to produce OTA, to correlate with meteorological conditions and geographical coordinates with their prevalence and to evaluate the OTA natural occurrence in grapes and wines. The morphological identification showed that Aspergillus niger aggregate species were the most prevalent ones, followed by Aspergillus carbonarius and Aspergillus uniseriate. These populations were confirmed through using AFLP markers and sequencing and, Aspergillus tubingensis was separated from A. niger aggregate. Climatic factors, altitude, longitude and latitude have influenced on the distribution of species included in the section. A. carbonarius and A. niger were OTA producers but differed in their OTA producing ability. Temperature was the factor which influenced the most over the highest incidence of A. carbonarius in La Rioja and San Juan regions. The trellis system in vineyards and drip irrigation also influenced the species isolation. The OTA levels detected in grapes and wines were low, but grape variety was more important in susceptibility to fungal infection and OTA levels. Fil: Chiotta, María Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Físico-Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ponsone, Marí­a Lorena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Sosa, Débora María. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Biología Molecular. Sección Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba; Argentina Fil: Combina, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Chulze, Sofia Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina 2017-10-06T12:24:38Z 2017-10-06T12:24:38Z 2013-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/1424 http://www.sciencedirect.com/science/article/pii/S0740002013000725 0740-0020 https://doi.org/10.1016/j.fm.2013.04.003 eng info:eu-repo/semantics/restrictedAccess application/pdf Argentina Food microbiology 36 (2) : 182-190. (Dec. 2013)
spellingShingle Vid
Grapevines
Wines
Mycotoxins
Identification
Ochratoxins
Vinos
Micotoxinas
Aspergillus
Identificación
Ocratoxinas
Argentina
Ochratoxin A
Agro-ecological Conditions
Aspergillus Section Nigri
Chiotta, María Laura
Ponsone, Marí­a Lorena
Sosa, Débora María
Combina, Mariana
Chulze, Sofia Noemí
Biodiversity of Aspergillus section Nigri populations in Argentinian vineyards and ochratoxin A contamination
title Biodiversity of Aspergillus section Nigri populations in Argentinian vineyards and ochratoxin A contamination
title_full Biodiversity of Aspergillus section Nigri populations in Argentinian vineyards and ochratoxin A contamination
title_fullStr Biodiversity of Aspergillus section Nigri populations in Argentinian vineyards and ochratoxin A contamination
title_full_unstemmed Biodiversity of Aspergillus section Nigri populations in Argentinian vineyards and ochratoxin A contamination
title_short Biodiversity of Aspergillus section Nigri populations in Argentinian vineyards and ochratoxin A contamination
title_sort biodiversity of aspergillus section nigri populations in argentinian vineyards and ochratoxin a contamination
topic Vid
Grapevines
Wines
Mycotoxins
Identification
Ochratoxins
Vinos
Micotoxinas
Aspergillus
Identificación
Ocratoxinas
Argentina
Ochratoxin A
Agro-ecological Conditions
Aspergillus Section Nigri
url http://hdl.handle.net/20.500.12123/1424
http://www.sciencedirect.com/science/article/pii/S0740002013000725
https://doi.org/10.1016/j.fm.2013.04.003
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