Target-site and non-target-site resistance mechanisms confer multiple resistance to glyphosate and 2,4-D in Carduus acanthoides
Carduus acanthoides L. is mainly a range-land weed, but in the 2010s has begun to invade GM crop production systems in Córdoba (Argentina), where glyphosate and 2,4-D have been commonly applied. In 2020, C. acanthoides was found with multiple resistance to these two herbicides. In this study, the me...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Artículo |
| Language: | Inglés |
| Published: |
Elsevier
2023
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/14182 https://www.sciencedirect.com/science/article/abs/pii/S0048357523000366 https://doi.org/10.1016/j.pestbp.2023.105371 |
| _version_ | 1855485317073600512 |
|---|---|
| author | Palma Bautista, Candelario Belluccini, Pablo Alberto Vázquez García, José Guadalupe Alcántara de la Cruz, Ricardo Barro, Francisco Portugal, João de Prado, Rafael |
| author_browse | Alcántara de la Cruz, Ricardo Barro, Francisco Belluccini, Pablo Alberto Palma Bautista, Candelario Portugal, João Vázquez García, José Guadalupe de Prado, Rafael |
| author_facet | Palma Bautista, Candelario Belluccini, Pablo Alberto Vázquez García, José Guadalupe Alcántara de la Cruz, Ricardo Barro, Francisco Portugal, João de Prado, Rafael |
| author_sort | Palma Bautista, Candelario |
| collection | INTA Digital |
| description | Carduus acanthoides L. is mainly a range-land weed, but in the 2010s has begun to invade GM crop production systems in Córdoba (Argentina), where glyphosate and 2,4-D have been commonly applied. In 2020, C. acanthoides was found with multiple resistance to these two herbicides. In this study, the mechanisms that confer multiple resistance to glyphosate and 2,4-D, were characterized in one resistant (R) population of C. acanthoides in comparison to a susceptible (S) population. No differences in 14C-herbicide absorption and translocation were observed between R and S populations. In addition, 14C-glyphosate was well translocated to the shoots (∼30%) and roots (∼16%) in both R and S plants, while most of 14C-2,4-D remained restricted in the treated leaf. Glyphosate metabolism did not contribute to resistance of the R population; however, as corroborated by malathion pretreatment, the mechanism of resistance to 2,4-D was enhanced metabolism (63% of the herbicide) mediated by cytochrome P450 (Cyt-P450). No differences were found in baseline EPSPS activity, copy number, and/or gene expression between the R and S populations, but a Pro-106-Ser mutation in EPSPS was present in the R population. Multiple resistances in the R population of C. acanthoides from Argentina were governed by target site resistance (a Pro-106 mutation for glyphosate) and non-target site resistance (Cyt-P450-based metabolic resistance for 2,4-D) mechanisms. This is the first case of resistance to glyphosate and 2,4-D confirmed for this weed in the world. |
| format | Artículo |
| id | INTA14182 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA141822023-03-08T14:16:15Z Target-site and non-target-site resistance mechanisms confer multiple resistance to glyphosate and 2,4-D in Carduus acanthoides Palma Bautista, Candelario Belluccini, Pablo Alberto Vázquez García, José Guadalupe Alcántara de la Cruz, Ricardo Barro, Francisco Portugal, João de Prado, Rafael Carduus acanthoides Glifosato Resistencia a los Herbicidas Malezas Glyphosate 2,4-D Herbicide Resistance Weeds 2,4-dichlorophenoxyacetic Acid Ácido 2,4-diclorofenoxiacético Carduus acanthoides L. is mainly a range-land weed, but in the 2010s has begun to invade GM crop production systems in Córdoba (Argentina), where glyphosate and 2,4-D have been commonly applied. In 2020, C. acanthoides was found with multiple resistance to these two herbicides. In this study, the mechanisms that confer multiple resistance to glyphosate and 2,4-D, were characterized in one resistant (R) population of C. acanthoides in comparison to a susceptible (S) population. No differences in 14C-herbicide absorption and translocation were observed between R and S populations. In addition, 14C-glyphosate was well translocated to the shoots (∼30%) and roots (∼16%) in both R and S plants, while most of 14C-2,4-D remained restricted in the treated leaf. Glyphosate metabolism did not contribute to resistance of the R population; however, as corroborated by malathion pretreatment, the mechanism of resistance to 2,4-D was enhanced metabolism (63% of the herbicide) mediated by cytochrome P450 (Cyt-P450). No differences were found in baseline EPSPS activity, copy number, and/or gene expression between the R and S populations, but a Pro-106-Ser mutation in EPSPS was present in the R population. Multiple resistances in the R population of C. acanthoides from Argentina were governed by target site resistance (a Pro-106 mutation for glyphosate) and non-target site resistance (Cyt-P450-based metabolic resistance for 2,4-D) mechanisms. This is the first case of resistance to glyphosate and 2,4-D confirmed for this weed in the world. EEA Marcos Juárez Fil: Palma Bautista, Candelario. Universidad de Córdoba. Department of Agroforestry, Plant Biochemistry and Molecular Biology; España Fil: Belluccini, Pablo Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina Fil: Vázquez García, José G. Universidad de Córdoba. Department of Agroforestry, Plant Biochemistry and Molecular Biology; España Fil: Alcántara de la Cruz, Ricardo. Universidade Federal de São Carlos. Centro de Ciências da Natureza; Brasil Fil: Barro, Francisco. CSIC. Institute for Sustainable Agriculture; España Fil: Portugal, João. Polytechnic Institute of Beja. Biosciences Department; Portugal Fil: Portugal, João. Polytechnic Institute of Portalegre. VALORIZA-Research Centre for Endogenous Resource Valorization; Portugal Fil: de Prado, Rafael. Universidad de Córdoba. Department of Agroforestry, Plant Biochemistry and Molecular Biology; España 2023-03-08T14:13:54Z 2023-03-08T14:13:54Z 2023-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/14182 https://www.sciencedirect.com/science/article/abs/pii/S0048357523000366 1095-9939 0048-3575 https://doi.org/10.1016/j.pestbp.2023.105371 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Pesticide Biochemistry and Physiology 191 : 105371 (April 2023) |
| spellingShingle | Carduus acanthoides Glifosato Resistencia a los Herbicidas Malezas Glyphosate 2,4-D Herbicide Resistance Weeds 2,4-dichlorophenoxyacetic Acid Ácido 2,4-diclorofenoxiacético Palma Bautista, Candelario Belluccini, Pablo Alberto Vázquez García, José Guadalupe Alcántara de la Cruz, Ricardo Barro, Francisco Portugal, João de Prado, Rafael Target-site and non-target-site resistance mechanisms confer multiple resistance to glyphosate and 2,4-D in Carduus acanthoides |
| title | Target-site and non-target-site resistance mechanisms confer multiple resistance to glyphosate and 2,4-D in Carduus acanthoides |
| title_full | Target-site and non-target-site resistance mechanisms confer multiple resistance to glyphosate and 2,4-D in Carduus acanthoides |
| title_fullStr | Target-site and non-target-site resistance mechanisms confer multiple resistance to glyphosate and 2,4-D in Carduus acanthoides |
| title_full_unstemmed | Target-site and non-target-site resistance mechanisms confer multiple resistance to glyphosate and 2,4-D in Carduus acanthoides |
| title_short | Target-site and non-target-site resistance mechanisms confer multiple resistance to glyphosate and 2,4-D in Carduus acanthoides |
| title_sort | target site and non target site resistance mechanisms confer multiple resistance to glyphosate and 2 4 d in carduus acanthoides |
| topic | Carduus acanthoides Glifosato Resistencia a los Herbicidas Malezas Glyphosate 2,4-D Herbicide Resistance Weeds 2,4-dichlorophenoxyacetic Acid Ácido 2,4-diclorofenoxiacético |
| url | http://hdl.handle.net/20.500.12123/14182 https://www.sciencedirect.com/science/article/abs/pii/S0048357523000366 https://doi.org/10.1016/j.pestbp.2023.105371 |
| work_keys_str_mv | AT palmabautistacandelario targetsiteandnontargetsiteresistancemechanismsconfermultipleresistancetoglyphosateand24dincarduusacanthoides AT belluccinipabloalberto targetsiteandnontargetsiteresistancemechanismsconfermultipleresistancetoglyphosateand24dincarduusacanthoides AT vazquezgarciajoseguadalupe targetsiteandnontargetsiteresistancemechanismsconfermultipleresistancetoglyphosateand24dincarduusacanthoides AT alcantaradelacruzricardo targetsiteandnontargetsiteresistancemechanismsconfermultipleresistancetoglyphosateand24dincarduusacanthoides AT barrofrancisco targetsiteandnontargetsiteresistancemechanismsconfermultipleresistancetoglyphosateand24dincarduusacanthoides AT portugaljoao targetsiteandnontargetsiteresistancemechanismsconfermultipleresistancetoglyphosateand24dincarduusacanthoides AT depradorafael targetsiteandnontargetsiteresistancemechanismsconfermultipleresistancetoglyphosateand24dincarduusacanthoides |