Construction of low-ethanol-wine yeasts through partial deletion of the Saccharomyces cerevisiae PDC2 gene

We propose an alternative GMO based strategy to obtain Saccharomyces cerevisiae mutant strains with a slight reduction in their ability to produce ethanol, but with a moderate impact on the yeast metabolism. Through homologous recombination, two truncated Pdc2p proteins Pdc2pΔ344 and Pdc2p Δ519 were...

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Autores principales: Cuello, Raúl Andrés, Flores Montero, Karina Johana, Mercado, Laura Analia, Combina, Mariana, Ciklic, Ivan Francisco
Formato: info:ar-repo/semantics/artículo
Lenguaje:Inglés
Publicado: 2017
Materias:
Acceso en línea:http://hdl.handle.net/20.500.12123/1410
https://amb-express.springeropen.com/track/pdf/10.1186/s13568-017-0369-2?site=amb-express.springeropen.com
https://doi.org/10.1186/s13568-017-0369-2
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author Cuello, Raúl Andrés
Flores Montero, Karina Johana
Mercado, Laura Analia
Combina, Mariana
Ciklic, Ivan Francisco
author_browse Ciklic, Ivan Francisco
Combina, Mariana
Cuello, Raúl Andrés
Flores Montero, Karina Johana
Mercado, Laura Analia
author_facet Cuello, Raúl Andrés
Flores Montero, Karina Johana
Mercado, Laura Analia
Combina, Mariana
Ciklic, Ivan Francisco
author_sort Cuello, Raúl Andrés
collection INTA Digital
description We propose an alternative GMO based strategy to obtain Saccharomyces cerevisiae mutant strains with a slight reduction in their ability to produce ethanol, but with a moderate impact on the yeast metabolism. Through homologous recombination, two truncated Pdc2p proteins Pdc2pΔ344 and Pdc2p Δ519 were obtained and transformed into haploid and diploid lab yeast strains. In the pdc2Δ344 mutants the DNA-binding and transactivation site of the protein remain intact, whereas in pdc2Δ519only the DNA-binding site is conserved. Compared to the control, the diploid BY4743 pdc2Δ519 mutant strain reduced up to 7.4% the total ethanol content in lab scale-vinifications. The residual sugar and volatile acidity was not significantly affected by this ethanol reduction. Remarkably, we got a much higher ethanol reduction of 10 and 15% when the pdc2Δ519 mutation was tested in a native and a commercial wine yeast strain against their respective controls. Our results demonstrate that the insertion of the pdc2Δ519 mutation in wine yeast strains can reduce the ethanol concentration up to 1.89% (v/v) without affecting the fermentation performance. In contrast to non-GMO based strategies, our approach permits the insertion of the pdc2Δ519 mutation in any locally selected wine strain, making possible to produce quality wines with regional characteristics and lower alcohol content. Thus, we consider our work a valuable contribution to the problem of high ethanol concentration in wine
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institution Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina)
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spelling INTA14102021-10-15T14:04:10Z Construction of low-ethanol-wine yeasts through partial deletion of the Saccharomyces cerevisiae PDC2 gene Cuello, Raúl Andrés Flores Montero, Karina Johana Mercado, Laura Analia Combina, Mariana Ciklic, Ivan Francisco Saccharomyces Cerevisiae Levadura Etanol Ingeniería Genética Vinos Yeasts Ethanol Genetic Engineering Wines We propose an alternative GMO based strategy to obtain Saccharomyces cerevisiae mutant strains with a slight reduction in their ability to produce ethanol, but with a moderate impact on the yeast metabolism. Through homologous recombination, two truncated Pdc2p proteins Pdc2pΔ344 and Pdc2p Δ519 were obtained and transformed into haploid and diploid lab yeast strains. In the pdc2Δ344 mutants the DNA-binding and transactivation site of the protein remain intact, whereas in pdc2Δ519only the DNA-binding site is conserved. Compared to the control, the diploid BY4743 pdc2Δ519 mutant strain reduced up to 7.4% the total ethanol content in lab scale-vinifications. The residual sugar and volatile acidity was not significantly affected by this ethanol reduction. Remarkably, we got a much higher ethanol reduction of 10 and 15% when the pdc2Δ519 mutation was tested in a native and a commercial wine yeast strain against their respective controls. Our results demonstrate that the insertion of the pdc2Δ519 mutation in wine yeast strains can reduce the ethanol concentration up to 1.89% (v/v) without affecting the fermentation performance. In contrast to non-GMO based strategies, our approach permits the insertion of the pdc2Δ519 mutation in any locally selected wine strain, making possible to produce quality wines with regional characteristics and lower alcohol content. Thus, we consider our work a valuable contribution to the problem of high ethanol concentration in wine EEA Mendoza Fil: Cuello, Raúl Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina. Fil: Flores Montero, Karina Johana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina Fil: Mercado, Laura Analia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina Fil: Combina, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina. Fil: Ciklic, Ivan Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina 2017-10-05T12:21:11Z 2017-10-05T12:21:11Z 2017 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/1410 https://amb-express.springeropen.com/track/pdf/10.1186/s13568-017-0369-2?site=amb-express.springeropen.com 2191-0855 (Online) https://doi.org/10.1186/s13568-017-0369-2 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf AMB Express 7 : 67 (2017)
spellingShingle Saccharomyces Cerevisiae
Levadura
Etanol
Ingeniería Genética
Vinos
Yeasts
Ethanol
Genetic Engineering
Wines
Cuello, Raúl Andrés
Flores Montero, Karina Johana
Mercado, Laura Analia
Combina, Mariana
Ciklic, Ivan Francisco
Construction of low-ethanol-wine yeasts through partial deletion of the Saccharomyces cerevisiae PDC2 gene
title Construction of low-ethanol-wine yeasts through partial deletion of the Saccharomyces cerevisiae PDC2 gene
title_full Construction of low-ethanol-wine yeasts through partial deletion of the Saccharomyces cerevisiae PDC2 gene
title_fullStr Construction of low-ethanol-wine yeasts through partial deletion of the Saccharomyces cerevisiae PDC2 gene
title_full_unstemmed Construction of low-ethanol-wine yeasts through partial deletion of the Saccharomyces cerevisiae PDC2 gene
title_short Construction of low-ethanol-wine yeasts through partial deletion of the Saccharomyces cerevisiae PDC2 gene
title_sort construction of low ethanol wine yeasts through partial deletion of the saccharomyces cerevisiae pdc2 gene
topic Saccharomyces Cerevisiae
Levadura
Etanol
Ingeniería Genética
Vinos
Yeasts
Ethanol
Genetic Engineering
Wines
url http://hdl.handle.net/20.500.12123/1410
https://amb-express.springeropen.com/track/pdf/10.1186/s13568-017-0369-2?site=amb-express.springeropen.com
https://doi.org/10.1186/s13568-017-0369-2
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