Morphological and molecular characterization of Nothopassalora personata from Argentina
Late leaf spot (LLS) is the most important foliar disease of peanuts in Argentina and in other peanut production areas, causing defoliation and yield losses. In this study, six isolates obtained from symptomatic leaflets collected in Córdoba, Argentina, were characterized based on the morphology of...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Springer
2023
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| Online Access: | http://hdl.handle.net/20.500.12123/14072 https://link.springer.com/article/10.1007/s42161-022-01292-y https://doi.org/10.1007/s42161-022-01292-y |
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| author | Monguillot, Joaquín Humberto Bernanrdi Lima, Nelson Paredes, Juan Andrés Giordano, Damián Francisco Oddino, Claudio Rago, Alejandro Mario Carmona, Marcelo Conforto, Erica Cinthia |
| author_browse | Bernanrdi Lima, Nelson Carmona, Marcelo Conforto, Erica Cinthia Giordano, Damián Francisco Monguillot, Joaquín Humberto Oddino, Claudio Paredes, Juan Andrés Rago, Alejandro Mario |
| author_facet | Monguillot, Joaquín Humberto Bernanrdi Lima, Nelson Paredes, Juan Andrés Giordano, Damián Francisco Oddino, Claudio Rago, Alejandro Mario Carmona, Marcelo Conforto, Erica Cinthia |
| author_sort | Monguillot, Joaquín Humberto |
| collection | INTA Digital |
| description | Late leaf spot (LLS) is the most important foliar disease of peanuts in Argentina and in other peanut production areas, causing defoliation and yield losses. In this study, six isolates obtained from symptomatic leaflets collected in Córdoba, Argentina, were characterized based on the morphology of the colony and conidia, as well as molecularly using a combination of the 28S nrRNA gene (LSU), the internal transcribed spacer regions and the intervening 5.8S nrRNA gene (ITS) of the rDNA operon, and the second largest subunit of RNA polymerase II (RPB2). Pathogenicity was confirmed using the detached leaf method, inoculating a spore suspension. Mycelial growth was evaluated on four culture media: peanut oatmeal agar (POA), potato dextrose agar (PDA), peanut leaflet extract agar (PLA) and peanut seed extract agar (PSA), with PSA and PDA showing the greatest radial growth. This is the first study of N. personata obtained in the peanut-producing region of Argentina. |
| format | info:ar-repo/semantics/artículo |
| id | INTA14072 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA140722023-02-27T10:07:21Z Morphological and molecular characterization of Nothopassalora personata from Argentina Monguillot, Joaquín Humberto Bernanrdi Lima, Nelson Paredes, Juan Andrés Giordano, Damián Francisco Oddino, Claudio Rago, Alejandro Mario Carmona, Marcelo Conforto, Erica Cinthia Mycosphaerella berkeleyi Arachis hypogaea Argentina Enfermedades de las Plantas Foliar Diseases Plant Diseases Enfermedades Foliares Cercosporidium Personatum Maní Late leaf spot (LLS) is the most important foliar disease of peanuts in Argentina and in other peanut production areas, causing defoliation and yield losses. In this study, six isolates obtained from symptomatic leaflets collected in Córdoba, Argentina, were characterized based on the morphology of the colony and conidia, as well as molecularly using a combination of the 28S nrRNA gene (LSU), the internal transcribed spacer regions and the intervening 5.8S nrRNA gene (ITS) of the rDNA operon, and the second largest subunit of RNA polymerase II (RPB2). Pathogenicity was confirmed using the detached leaf method, inoculating a spore suspension. Mycelial growth was evaluated on four culture media: peanut oatmeal agar (POA), potato dextrose agar (PDA), peanut leaflet extract agar (PLA) and peanut seed extract agar (PSA), with PSA and PDA showing the greatest radial growth. This is the first study of N. personata obtained in the peanut-producing region of Argentina. Instituto de Patología Vegetal Fil: Monguillot, Joaquín Humberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina Fil: Monguillot, Joaquín Humberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Bernanrdi Lima, Nelson. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina Fil: Bernanrdi Lima, Nelson. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Bernanrdi Lima, Nelson. Universidad Nacional de Catamarca ( UNCa). Facultad de Ciencias Agrarias; Argentina Fil: Paredes, Juan Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina Fil: Paredes, Juan Andrés. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Giordano, Damián Francisco. Universidad Nacional de Río Cuarto (UNRC). Facultad de Agronomía y Veterinaria. Instituto de Investigación en Micología y Micotoxicología (IMICO); Argentina Fil: Oddino, Claudio. Universidad Nacional de Río Cuarto ( UNRC). Facultad de Agronomía y Veterinaria (FAV).Instituto de Investigación en Micología y Micotoxicología (IMICO); Argentina Fil: Rago, Alejandro Mario. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigaciones Agropecuarias; Argentina Fil: Rago, Alejandro Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina Fil: Carmona, Marcelo. Universidad de Buenos Aires. Facultad de Agronomía. Catedra de Fitopatología; Argentina Fil: Conforto, Erica Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Conforto, Erica Cinthia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina 2023-02-27T10:01:29Z 2023-02-27T10:01:29Z 2023-01-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/14072 https://link.springer.com/article/10.1007/s42161-022-01292-y 1125-4653 2239-7264 (online) https://doi.org/10.1007/s42161-022-01292-y eng info:eu-repograntAgreement/INTA/2019-PE-E4-I086-001/2019-PE-E4-I086-001/AR./Monitoreo, caracterización y manejo de plagas resistentes a fitosanitarios y organismos geneticamente modificados info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Journal of Plant Pathology (2023) (Published: 10 January 2023) |
| spellingShingle | Mycosphaerella berkeleyi Arachis hypogaea Argentina Enfermedades de las Plantas Foliar Diseases Plant Diseases Enfermedades Foliares Cercosporidium Personatum Maní Monguillot, Joaquín Humberto Bernanrdi Lima, Nelson Paredes, Juan Andrés Giordano, Damián Francisco Oddino, Claudio Rago, Alejandro Mario Carmona, Marcelo Conforto, Erica Cinthia Morphological and molecular characterization of Nothopassalora personata from Argentina |
| title | Morphological and molecular characterization of Nothopassalora personata from Argentina |
| title_full | Morphological and molecular characterization of Nothopassalora personata from Argentina |
| title_fullStr | Morphological and molecular characterization of Nothopassalora personata from Argentina |
| title_full_unstemmed | Morphological and molecular characterization of Nothopassalora personata from Argentina |
| title_short | Morphological and molecular characterization of Nothopassalora personata from Argentina |
| title_sort | morphological and molecular characterization of nothopassalora personata from argentina |
| topic | Mycosphaerella berkeleyi Arachis hypogaea Argentina Enfermedades de las Plantas Foliar Diseases Plant Diseases Enfermedades Foliares Cercosporidium Personatum Maní |
| url | http://hdl.handle.net/20.500.12123/14072 https://link.springer.com/article/10.1007/s42161-022-01292-y https://doi.org/10.1007/s42161-022-01292-y |
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