Novel yeast isolated from broilers feedstuff, gut and faeces as aflatoxin 1 B1 adsorbents
Aims: To isolate and characterize native yeast strains from broilers’ environment as feedstuff, faeces and gut, and to evaluate their binding capacity for aflatoxin B1 (AFB1). Methods and Results: A total of nine yeast strains were isolated: three from feedstuff identified as Pichia kudriavzevii...
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| Format: | info:eu-repo/semantics/article |
| Language: | Inglés |
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2017
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| Online Access: | http://hdl.handle.net/20.500.12123/1401 http://onlinelibrary.wiley.com/doi/10.1111/jam.13297/abstract |
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| author | Magnoli, Alejandra Paola Rodriguez Romera, Mariela Carmen Poloni, V.L. Combina, Mariana Chiacchiera, Sebastián Dalcero, Ana María Cavaglieri, Lilia Reneé Rojo, María Cecilia |
| author_browse | Cavaglieri, Lilia Reneé Chiacchiera, Sebastián Combina, Mariana Dalcero, Ana María Magnoli, Alejandra Paola Poloni, V.L. Rodriguez Romera, Mariela Carmen Rojo, María Cecilia |
| author_facet | Magnoli, Alejandra Paola Rodriguez Romera, Mariela Carmen Poloni, V.L. Combina, Mariana Chiacchiera, Sebastián Dalcero, Ana María Cavaglieri, Lilia Reneé Rojo, María Cecilia |
| author_sort | Magnoli, Alejandra Paola |
| collection | INTA Digital |
| description | Aims: To isolate and characterize native yeast strains from broilers’
environment as feedstuff, faeces and gut, and to evaluate their binding capacity for aflatoxin B1 (AFB1).
Methods and Results: A total of nine yeast strains were isolated: three from
feedstuff identified as Pichia kudriavzevii (2) and Clavispora lusitaniae (1), two from gut identified as Candida tropicalis and four from faeces identified as Cl. lusitaniae (3) and Cyberlindnera fabianii (1). AFB1 binding percentages varied among yeast strains and with AFB1 concentrations. To carry out adsorption studies, one strain from each genus and each origin was selected as follows: Cl. lusitaniae and P. kudriavzevii from feedstuff, Cl. lusitaniae and Cy. fabianii from faeces and Ca. tropicalis from gut. The most appropriate concentrations for cells and toxin were 107 cells per ml and 100 ng ml _1 of AFB1 respectively. All the tested yeast strains showed similar adsorption capacities independently of the origin. The adsorption isotherm studies in all
yeasts assayed showed behaviour of L type or Langmuir and a varied affinity for the toxin. The stability of the AFB1–yeast complex demonstrated the irreversibility of the binding process. Conclusion: Yeast strains tested in this study constitute potential AFB1 adsorbents and they possess the advantage to be native from the avian environment.
Significance and Impact of the Study: This study makes a contribution to
using native yeasts from broilers’ environment for controlling chronic
aflatoxicosis in avian production. |
| format | info:eu-repo/semantics/article |
| id | INTA1401 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA14012018-01-12T18:55:07Z Novel yeast isolated from broilers feedstuff, gut and faeces as aflatoxin 1 B1 adsorbents Magnoli, Alejandra Paola Rodriguez Romera, Mariela Carmen Poloni, V.L. Combina, Mariana Chiacchiera, Sebastián Dalcero, Ana María Cavaglieri, Lilia Reneé Rojo, María Cecilia Pollo de Engorde Levadura Piensos Aflatoxinas Broiler Chickens Yeasts Feeds Aflatoxins Aims: To isolate and characterize native yeast strains from broilers’ environment as feedstuff, faeces and gut, and to evaluate their binding capacity for aflatoxin B1 (AFB1). Methods and Results: A total of nine yeast strains were isolated: three from feedstuff identified as Pichia kudriavzevii (2) and Clavispora lusitaniae (1), two from gut identified as Candida tropicalis and four from faeces identified as Cl. lusitaniae (3) and Cyberlindnera fabianii (1). AFB1 binding percentages varied among yeast strains and with AFB1 concentrations. To carry out adsorption studies, one strain from each genus and each origin was selected as follows: Cl. lusitaniae and P. kudriavzevii from feedstuff, Cl. lusitaniae and Cy. fabianii from faeces and Ca. tropicalis from gut. The most appropriate concentrations for cells and toxin were 107 cells per ml and 100 ng ml _1 of AFB1 respectively. All the tested yeast strains showed similar adsorption capacities independently of the origin. The adsorption isotherm studies in all yeasts assayed showed behaviour of L type or Langmuir and a varied affinity for the toxin. The stability of the AFB1–yeast complex demonstrated the irreversibility of the binding process. Conclusion: Yeast strains tested in this study constitute potential AFB1 adsorbents and they possess the advantage to be native from the avian environment. Significance and Impact of the Study: This study makes a contribution to using native yeasts from broilers’ environment for controlling chronic aflatoxicosis in avian production. EEA Mendoza Fil: Magnoli, Alejandra Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina Fil: Rodriguez Romera, Mariela Carmen. Universidad Nacional de Río Cuarto. Departamento de Microbiología e Inmunología; Argentina Fil: Poloni, V.L. Universidad Nacional de Río Cuarto. Departamento de Microbiología e Inmunología; Argentina Fil: Combina, Mariana.Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Centro de Estudios Enológicos; Argentina Fil: Chiacchiera, Sebastián. Estación Experimental Agropecuaria Marcos Juarez; Argentina Fil: Dalcero, Ana Maria. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cavaglieri, Lilia Reneé. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Rojo, María Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2017-10-04T13:11:25Z 2017-10-04T13:11:25Z 2016 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion info:ar-repo/semantics/artículo http://hdl.handle.net/20.500.12123/1401 http://onlinelibrary.wiley.com/doi/10.1111/jam.13297/abstract 1364-5072 (Print) 1365-2672 (Online) DOI: 10.1111/jam.13297 eng info:eu-repo/semantics/restrictedAccess application/pdf Journal of applied microbiology 121 (6) : 1766–1776. (December 2016) |
| spellingShingle | Pollo de Engorde Levadura Piensos Aflatoxinas Broiler Chickens Yeasts Feeds Aflatoxins Magnoli, Alejandra Paola Rodriguez Romera, Mariela Carmen Poloni, V.L. Combina, Mariana Chiacchiera, Sebastián Dalcero, Ana María Cavaglieri, Lilia Reneé Rojo, María Cecilia Novel yeast isolated from broilers feedstuff, gut and faeces as aflatoxin 1 B1 adsorbents |
| title | Novel yeast isolated from broilers feedstuff, gut and faeces as aflatoxin 1 B1 adsorbents |
| title_full | Novel yeast isolated from broilers feedstuff, gut and faeces as aflatoxin 1 B1 adsorbents |
| title_fullStr | Novel yeast isolated from broilers feedstuff, gut and faeces as aflatoxin 1 B1 adsorbents |
| title_full_unstemmed | Novel yeast isolated from broilers feedstuff, gut and faeces as aflatoxin 1 B1 adsorbents |
| title_short | Novel yeast isolated from broilers feedstuff, gut and faeces as aflatoxin 1 B1 adsorbents |
| title_sort | novel yeast isolated from broilers feedstuff gut and faeces as aflatoxin 1 b1 adsorbents |
| topic | Pollo de Engorde Levadura Piensos Aflatoxinas Broiler Chickens Yeasts Feeds Aflatoxins |
| url | http://hdl.handle.net/20.500.12123/1401 http://onlinelibrary.wiley.com/doi/10.1111/jam.13297/abstract |
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