ABA and GA 3 increase carbon allocation in different organs of grapevine plants by inducing accumulation of non-structural carbohydrates in leaves, enhancement of phloem area and expression of sugar transporters
Grape quality for winemaking depends on sugar accumulation and metabolism in berries. Abscisic acid (ABA) and gibberellins (GAs) have been reported to control sugar allocation in economically important crops, although the mechanisms involved are still unknown. The present study tested if ABA and gib...
| Autores principales: | , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/1397 http://onlinelibrary.wiley.com/doi/10.1111/ppl.12390/ https://doi.org/10.1111/ppl.12390 |
| _version_ | 1855482881164443648 |
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| author | Murcia, Mauro German Pontin, Mariela Ana Reinoso, Herminda Baraldi, Rita Bertazza, Gianpaolo Gomez Talquenca, Gonzalo Piccoli, Patricia Noemí Bottini, Ambrosio Rubén |
| author_browse | Baraldi, Rita Bertazza, Gianpaolo Bottini, Ambrosio Rubén Gomez Talquenca, Gonzalo Murcia, Mauro German Piccoli, Patricia Noemí Pontin, Mariela Ana Reinoso, Herminda |
| author_facet | Murcia, Mauro German Pontin, Mariela Ana Reinoso, Herminda Baraldi, Rita Bertazza, Gianpaolo Gomez Talquenca, Gonzalo Piccoli, Patricia Noemí Bottini, Ambrosio Rubén |
| author_sort | Murcia, Mauro German |
| collection | INTA Digital |
| description | Grape quality for winemaking depends on sugar accumulation and metabolism in berries. Abscisic acid (ABA) and gibberellins (GAs) have been reported to control sugar allocation in economically important crops, although the mechanisms involved are still unknown. The present study tested if ABA and gibberellin A3 (GA3) enhance carbon allocation in fruits of grapevines by modifying phloem loading, phloem area and expression of sugar transporters in leaves and berries. Pot-grown Vitis vinifera cv. Malbec
plants were sprayed with ABA and GA3 solutions. The amount of soluble sugars in leaves and berries related to photosynthesis were examined at three points of berry growth: pre-veraison, full veraison and post-veraison. Starch levels and amylase activity in leaves, gene expression of sugar transporters in leaves and berries and phloem anatomy were examined at full veraison. Accumulation of glucose and fructose in berries was hastened in ABA-treated plants at the stage of full veraison, which was correlated with enhancement of Vitis vinifera HEXOSE TRANSPORTER 2 (VvHT2) and Vitis vinifera HEXOSE TRANSPORTER 6 (VvHT6) gene expression, increases of phloem area and sucrose content in leaves. On the other hand, GA3 increased the quantity of photoassimilates delivered to the stem thus increasing xylem growth. In conclusion, stimulation of sugar transport by ABA and GA3 to berries and stems, respectively, was due to build-up of non-structural carbohydrates in leaves, modifications in phloem tissue and modulation in gene expression of sugar transporters. |
| format | Artículo |
| id | INTA1397 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA13972024-04-15T13:10:55Z ABA and GA 3 increase carbon allocation in different organs of grapevine plants by inducing accumulation of non-structural carbohydrates in leaves, enhancement of phloem area and expression of sugar transporters Murcia, Mauro German Pontin, Mariela Ana Reinoso, Herminda Baraldi, Rita Bertazza, Gianpaolo Gomez Talquenca, Gonzalo Piccoli, Patricia Noemí Bottini, Ambrosio Rubén Vid Vinificación ABA Floema Grapevines Winemaking Phloem Grape quality for winemaking depends on sugar accumulation and metabolism in berries. Abscisic acid (ABA) and gibberellins (GAs) have been reported to control sugar allocation in economically important crops, although the mechanisms involved are still unknown. The present study tested if ABA and gibberellin A3 (GA3) enhance carbon allocation in fruits of grapevines by modifying phloem loading, phloem area and expression of sugar transporters in leaves and berries. Pot-grown Vitis vinifera cv. Malbec plants were sprayed with ABA and GA3 solutions. The amount of soluble sugars in leaves and berries related to photosynthesis were examined at three points of berry growth: pre-veraison, full veraison and post-veraison. Starch levels and amylase activity in leaves, gene expression of sugar transporters in leaves and berries and phloem anatomy were examined at full veraison. Accumulation of glucose and fructose in berries was hastened in ABA-treated plants at the stage of full veraison, which was correlated with enhancement of Vitis vinifera HEXOSE TRANSPORTER 2 (VvHT2) and Vitis vinifera HEXOSE TRANSPORTER 6 (VvHT6) gene expression, increases of phloem area and sucrose content in leaves. On the other hand, GA3 increased the quantity of photoassimilates delivered to the stem thus increasing xylem growth. In conclusion, stimulation of sugar transport by ABA and GA3 to berries and stems, respectively, was due to build-up of non-structural carbohydrates in leaves, modifications in phloem tissue and modulation in gene expression of sugar transporters. EEA Mendoza Fil: Murcia, Mauro Germán. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Pontin, Mariela Ana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Baraldi, Rita. Consiglio Nazionale delle Ricerche. Istituto di Biometeorologia; Italia Fil: Bertazza, Gianpaolo. Consiglio Nazionale delle Ricerche. Istituto di Biometeorologia; Italia Fil: Reinoso, Herminda. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Ciencias Naturales; Argentina Fil: Gomez Talquenca, Gonzalo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Departamento de Virología; Argentina Fil: Bottini, Ambrosio Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Piccoli, Patricia Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina 2017-10-04T12:09:09Z 2017-10-04T12:09:09Z 2016 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/1397 http://onlinelibrary.wiley.com/doi/10.1111/ppl.12390/ 0031-9317 (Print) 1399-3054 (Online) https://doi.org/10.1111/ppl.12390 eng info:eu-repo/semantics/restrictedAccess application/pdf Physiologia plantarum 156 (3) : 323–337. (March 2016) |
| spellingShingle | Vid Vinificación ABA Floema Grapevines Winemaking Phloem Murcia, Mauro German Pontin, Mariela Ana Reinoso, Herminda Baraldi, Rita Bertazza, Gianpaolo Gomez Talquenca, Gonzalo Piccoli, Patricia Noemí Bottini, Ambrosio Rubén ABA and GA 3 increase carbon allocation in different organs of grapevine plants by inducing accumulation of non-structural carbohydrates in leaves, enhancement of phloem area and expression of sugar transporters |
| title | ABA and GA 3 increase carbon allocation in different organs of grapevine plants by inducing accumulation of non-structural carbohydrates in leaves, enhancement of phloem area and expression of sugar transporters |
| title_full | ABA and GA 3 increase carbon allocation in different organs of grapevine plants by inducing accumulation of non-structural carbohydrates in leaves, enhancement of phloem area and expression of sugar transporters |
| title_fullStr | ABA and GA 3 increase carbon allocation in different organs of grapevine plants by inducing accumulation of non-structural carbohydrates in leaves, enhancement of phloem area and expression of sugar transporters |
| title_full_unstemmed | ABA and GA 3 increase carbon allocation in different organs of grapevine plants by inducing accumulation of non-structural carbohydrates in leaves, enhancement of phloem area and expression of sugar transporters |
| title_short | ABA and GA 3 increase carbon allocation in different organs of grapevine plants by inducing accumulation of non-structural carbohydrates in leaves, enhancement of phloem area and expression of sugar transporters |
| title_sort | aba and ga 3 increase carbon allocation in different organs of grapevine plants by inducing accumulation of non structural carbohydrates in leaves enhancement of phloem area and expression of sugar transporters |
| topic | Vid Vinificación ABA Floema Grapevines Winemaking Phloem |
| url | http://hdl.handle.net/20.500.12123/1397 http://onlinelibrary.wiley.com/doi/10.1111/ppl.12390/ https://doi.org/10.1111/ppl.12390 |
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