Use of adjuvant ISA VG 71 to produce neutralizing egg yolk antibodies against bothropic venom
The use of egg yolk antibodies—IgY technology—represents an alternative to the production of mammalian immunoglobulins and has several advantages regarding animal welfare and lower costs of production. The use of adjuvants to achieve the hyperimmunization of laying hens plays a key role in the succe...
| Autores principales: | , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2023
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/13956 https://link.springer.com/article/10.1007/s00253-023-12409-3 https://doi.org/10.1007/s00253-023-12409-3 |
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| author | Leiva, Carlos Leónidas Cangelosi, Adriana Mariconda, Virginia Celi Castillo, Ana Beatriz Joaquim, Patricia Estefania Geoghegan, Patricia Fernandez Miyakawa, Mariano Enrique Chacana, Pablo |
| author_browse | Cangelosi, Adriana Celi Castillo, Ana Beatriz Chacana, Pablo Fernandez Miyakawa, Mariano Enrique Geoghegan, Patricia Joaquim, Patricia Estefania Leiva, Carlos Leónidas Mariconda, Virginia |
| author_facet | Leiva, Carlos Leónidas Cangelosi, Adriana Mariconda, Virginia Celi Castillo, Ana Beatriz Joaquim, Patricia Estefania Geoghegan, Patricia Fernandez Miyakawa, Mariano Enrique Chacana, Pablo |
| author_sort | Leiva, Carlos Leónidas |
| collection | INTA Digital |
| description | The use of egg yolk antibodies—IgY technology—represents an alternative to the production of mammalian immunoglobulins and has several advantages regarding animal welfare and lower costs of production. The use of adjuvants to achieve the hyperimmunization of laying hens plays a key role in the success of the production of high levels of the antibodies. In the present work, two different adjuvant systems (Freund’s adjuvants and MontanideTM ISA 71 VG) were compared to produce IgY anti-Bothrops alternatus. For the first immunization, formalin-inactivated Salmonella was added to MontanideTM ISA 71 VG to emulate Freund’s complete adjuvant which includes a mycobacteria antigen. After eight immunizations, IgY produced by using either adjuvant was able to neutralize the lethal activity of the venom in a mouse model, but differences were found regarding the recognition of components of the venom between the two adjuvants tested. Overall, MontanideTM adjuvant used in this work could be a good alternative choice to produce antibodies capable of neutralizing the lethality of complex antigens. This adjuvant is commercially available and used in the formulation of several poultry vaccines and could be used for the IgY technology instead of traditional immunomodulators such as Freund’s adjuvants. |
| format | info:ar-repo/semantics/artículo |
| id | INTA13956 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA139562023-02-13T14:12:43Z Use of adjuvant ISA VG 71 to produce neutralizing egg yolk antibodies against bothropic venom Leiva, Carlos Leónidas Cangelosi, Adriana Mariconda, Virginia Celi Castillo, Ana Beatriz Joaquim, Patricia Estefania Geoghegan, Patricia Fernandez Miyakawa, Mariano Enrique Chacana, Pablo Egg Yolk Adjuvants Mass Spectrometry Antibodies Neutralization Yema de Huevo Coadyuvantes Espectrometría de Masas Anticuerpos Neutralización IgY Antibodies Anticuerpos IgY The use of egg yolk antibodies—IgY technology—represents an alternative to the production of mammalian immunoglobulins and has several advantages regarding animal welfare and lower costs of production. The use of adjuvants to achieve the hyperimmunization of laying hens plays a key role in the success of the production of high levels of the antibodies. In the present work, two different adjuvant systems (Freund’s adjuvants and MontanideTM ISA 71 VG) were compared to produce IgY anti-Bothrops alternatus. For the first immunization, formalin-inactivated Salmonella was added to MontanideTM ISA 71 VG to emulate Freund’s complete adjuvant which includes a mycobacteria antigen. After eight immunizations, IgY produced by using either adjuvant was able to neutralize the lethal activity of the venom in a mouse model, but differences were found regarding the recognition of components of the venom between the two adjuvants tested. Overall, MontanideTM adjuvant used in this work could be a good alternative choice to produce antibodies capable of neutralizing the lethality of complex antigens. This adjuvant is commercially available and used in the formulation of several poultry vaccines and could be used for the IgY technology instead of traditional immunomodulators such as Freund’s adjuvants. Instituto de Patobiología Fil: Leiva, Carlos Leónidas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Leiva, Carlos Leónidas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cangelosi, Adriana. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentina Fil: Mariconda, Virginia. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentina Fil: Celi Castillo, Ana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Celi Castillo, Ana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Joaquim, Patricia Estefania. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Joaquim, Patricia Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Geoghegan, Patricia. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentina Fil: Fernandez Miyakawa, Mariano Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Fernandez Miyakawa, Mariano Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Chacana, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Chacana, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2023-02-13T14:05:17Z 2023-02-13T14:05:17Z 2023-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/13956 https://link.springer.com/article/10.1007/s00253-023-12409-3 1432-0614 0175-7598 https://doi.org/10.1007/s00253-023-12409-3 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Applied Microbiology and Biotechnology (Published: 01 February 2023) |
| spellingShingle | Egg Yolk Adjuvants Mass Spectrometry Antibodies Neutralization Yema de Huevo Coadyuvantes Espectrometría de Masas Anticuerpos Neutralización IgY Antibodies Anticuerpos IgY Leiva, Carlos Leónidas Cangelosi, Adriana Mariconda, Virginia Celi Castillo, Ana Beatriz Joaquim, Patricia Estefania Geoghegan, Patricia Fernandez Miyakawa, Mariano Enrique Chacana, Pablo Use of adjuvant ISA VG 71 to produce neutralizing egg yolk antibodies against bothropic venom |
| title | Use of adjuvant ISA VG 71 to produce neutralizing egg yolk antibodies against bothropic venom |
| title_full | Use of adjuvant ISA VG 71 to produce neutralizing egg yolk antibodies against bothropic venom |
| title_fullStr | Use of adjuvant ISA VG 71 to produce neutralizing egg yolk antibodies against bothropic venom |
| title_full_unstemmed | Use of adjuvant ISA VG 71 to produce neutralizing egg yolk antibodies against bothropic venom |
| title_short | Use of adjuvant ISA VG 71 to produce neutralizing egg yolk antibodies against bothropic venom |
| title_sort | use of adjuvant isa vg 71 to produce neutralizing egg yolk antibodies against bothropic venom |
| topic | Egg Yolk Adjuvants Mass Spectrometry Antibodies Neutralization Yema de Huevo Coadyuvantes Espectrometría de Masas Anticuerpos Neutralización IgY Antibodies Anticuerpos IgY |
| url | http://hdl.handle.net/20.500.12123/13956 https://link.springer.com/article/10.1007/s00253-023-12409-3 https://doi.org/10.1007/s00253-023-12409-3 |
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