Midgut Genes Knockdown by Oral dsRNA Administration Produces a Lethal Effect on Cotton Boll Weevil
The “cotton boll weevil” (Anthonomus grandis Boheman) is a key pest in America whose larval stage develops within the cotton flower bud. During its development, the larva uses the flower bud as food and as a shelter from predators. This behavior limits the effective control through conventional inse...
| Main Authors: | , , , , |
|---|---|
| Format: | Artículo |
| Language: | Inglés |
| Published: |
Springer
2023
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/13949 https://link.springer.com/article/10.1007/s13744-020-00819-1 https://doi.org/10.1007/s13744-020-00819-1 |
| _version_ | 1855485280050479104 |
|---|---|
| author | Salvador, Ricardo Niz, José María Nakaya, Pablo Angel Pedarros, Analía Soledad Hopp, Horacio Esteban |
| author_browse | Hopp, Horacio Esteban Nakaya, Pablo Angel Niz, José María Pedarros, Analía Soledad Salvador, Ricardo |
| author_facet | Salvador, Ricardo Niz, José María Nakaya, Pablo Angel Pedarros, Analía Soledad Hopp, Horacio Esteban |
| author_sort | Salvador, Ricardo |
| collection | INTA Digital |
| description | The “cotton boll weevil” (Anthonomus grandis Boheman) is a key pest in America whose larval stage develops within the cotton flower bud. During its development, the larva uses the flower bud as food and as a shelter from predators. This behavior limits the effective control through conventional insecticide applications and biocontrol techniques. Increasing genetic information from insects has allowed the development of new control technologies based on the use of RNA interference (RNAi) to design orally delivered double-stranded RNA (dsRNA) strategies. In this study, we evaluated the effect of continuous oral administration of six specific dsRNA in order to identify an effective target gene for RNAi-mediated control of cotton boll weevil. First, six selected A. grandis gene fragments were amplified and cloned to perform in vivo synthesis of the specific dsRNA, and subsequently, larvae and adults were fed with this dsRNA for 2 weeks. Larvae mortality ranged from 40 to 60% depending on the targeted gene sequence. Indeed, α-amylase and cytochrome p450 dsRNAs were the most effective. Oral administration in adults caused smaller but still significant death rates (15–30%). Thus, the results demonstrated RNAi responses depend on life stages and target genes. The dsRNA ingestion was capable of providing knockdown mRNA levels in cotton boll weevil midgut and this effect was significantly higher in the larval stage. In this study, we present a new report of silencing of midgut genes in A. grandis larva induced by continuously feeding with dsRNA. This potential new tool should be further evaluated in cotton boll weevil control strategies. |
| format | Artículo |
| id | INTA13949 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA139492023-02-13T12:29:46Z Midgut Genes Knockdown by Oral dsRNA Administration Produces a Lethal Effect on Cotton Boll Weevil Salvador, Ricardo Niz, José María Nakaya, Pablo Angel Pedarros, Analía Soledad Hopp, Horacio Esteban RNA Gene Silencing Pest Control Cotton Crops Control de Plagas Anthonomus grandis Silenciamiento Génico Algodón ARN Cultivos Curculionidae Picudo del Algodonero The “cotton boll weevil” (Anthonomus grandis Boheman) is a key pest in America whose larval stage develops within the cotton flower bud. During its development, the larva uses the flower bud as food and as a shelter from predators. This behavior limits the effective control through conventional insecticide applications and biocontrol techniques. Increasing genetic information from insects has allowed the development of new control technologies based on the use of RNA interference (RNAi) to design orally delivered double-stranded RNA (dsRNA) strategies. In this study, we evaluated the effect of continuous oral administration of six specific dsRNA in order to identify an effective target gene for RNAi-mediated control of cotton boll weevil. First, six selected A. grandis gene fragments were amplified and cloned to perform in vivo synthesis of the specific dsRNA, and subsequently, larvae and adults were fed with this dsRNA for 2 weeks. Larvae mortality ranged from 40 to 60% depending on the targeted gene sequence. Indeed, α-amylase and cytochrome p450 dsRNAs were the most effective. Oral administration in adults caused smaller but still significant death rates (15–30%). Thus, the results demonstrated RNAi responses depend on life stages and target genes. The dsRNA ingestion was capable of providing knockdown mRNA levels in cotton boll weevil midgut and this effect was significantly higher in the larval stage. In this study, we present a new report of silencing of midgut genes in A. grandis larva induced by continuously feeding with dsRNA. This potential new tool should be further evaluated in cotton boll weevil control strategies. Instituto de Microbiología y Zoología Agrícola (IMYZA) Fil: Salvador, Ricardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Niz, José María. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Nakaya, Pablo Ángel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina Fil: Pedarros, Analía. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Hopp, Horacio Esteban. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Laboratorio de Agrobiotecnología; Argentina Fil: Hopp, Horacio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2023-02-13T12:20:42Z 2023-02-13T12:20:42Z 2021-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/13949 https://link.springer.com/article/10.1007/s13744-020-00819-1 1678-8052 https://doi.org/10.1007/s13744-020-00819-1 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Neotropical Entomology 50 : 121-128 (Febrero 2021) |
| spellingShingle | RNA Gene Silencing Pest Control Cotton Crops Control de Plagas Anthonomus grandis Silenciamiento Génico Algodón ARN Cultivos Curculionidae Picudo del Algodonero Salvador, Ricardo Niz, José María Nakaya, Pablo Angel Pedarros, Analía Soledad Hopp, Horacio Esteban Midgut Genes Knockdown by Oral dsRNA Administration Produces a Lethal Effect on Cotton Boll Weevil |
| title | Midgut Genes Knockdown by Oral dsRNA Administration Produces a Lethal Effect on Cotton Boll Weevil |
| title_full | Midgut Genes Knockdown by Oral dsRNA Administration Produces a Lethal Effect on Cotton Boll Weevil |
| title_fullStr | Midgut Genes Knockdown by Oral dsRNA Administration Produces a Lethal Effect on Cotton Boll Weevil |
| title_full_unstemmed | Midgut Genes Knockdown by Oral dsRNA Administration Produces a Lethal Effect on Cotton Boll Weevil |
| title_short | Midgut Genes Knockdown by Oral dsRNA Administration Produces a Lethal Effect on Cotton Boll Weevil |
| title_sort | midgut genes knockdown by oral dsrna administration produces a lethal effect on cotton boll weevil |
| topic | RNA Gene Silencing Pest Control Cotton Crops Control de Plagas Anthonomus grandis Silenciamiento Génico Algodón ARN Cultivos Curculionidae Picudo del Algodonero |
| url | http://hdl.handle.net/20.500.12123/13949 https://link.springer.com/article/10.1007/s13744-020-00819-1 https://doi.org/10.1007/s13744-020-00819-1 |
| work_keys_str_mv | AT salvadorricardo midgutgenesknockdownbyoraldsrnaadministrationproducesalethaleffectoncottonbollweevil AT nizjosemaria midgutgenesknockdownbyoraldsrnaadministrationproducesalethaleffectoncottonbollweevil AT nakayapabloangel midgutgenesknockdownbyoraldsrnaadministrationproducesalethaleffectoncottonbollweevil AT pedarrosanaliasoledad midgutgenesknockdownbyoraldsrnaadministrationproducesalethaleffectoncottonbollweevil AT hopphoracioesteban midgutgenesknockdownbyoraldsrnaadministrationproducesalethaleffectoncottonbollweevil |