Signs of a phyllospheric lifestyle in the genome of the stress-tolerant strain Azospirillum brasilense Az19
Azospirillum brasilense Az19 is a plant-beneficial bacterium capable of protecting plants from the negative effects of drought. The objective of this study was to determine and analyze the genomic sequence of strain Az19 as a means of identifying putative stress-adaptation mechanisms. A high-qualit...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2023
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/13916 https://www.sciencedirect.com/science/article/abs/pii/S0723202020300850 https://doi.org/10.1016/j.syapm.2020.126130 |
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| author | Garcia, Julia Elena Labarthe, María Mercedes Pagnussat, Luciana Anabella Amenta, Melina Beatriz Creus, Cecilia Mónica Maroniche, Guillermo Andrés |
| author_browse | Amenta, Melina Beatriz Creus, Cecilia Mónica Garcia, Julia Elena Labarthe, María Mercedes Maroniche, Guillermo Andrés Pagnussat, Luciana Anabella |
| author_facet | Garcia, Julia Elena Labarthe, María Mercedes Pagnussat, Luciana Anabella Amenta, Melina Beatriz Creus, Cecilia Mónica Maroniche, Guillermo Andrés |
| author_sort | Garcia, Julia Elena |
| collection | INTA Digital |
| description | Azospirillum brasilense Az19 is a plant-beneficial bacterium capable of protecting plants from the negative effects of drought. The objective of this study was to determine and analyze the genomic sequence of strain Az19 as a means of identifying putative stress-adaptation mechanisms. A high-quality draft genome of ca. 7 Mb with a predicted coding potential of 6710 genes was obtained. Phylogenomic analyses confirmed that Az19 belongs to the brasilense clade and is closely related to strains Az39 and REC3. Functional genomics revealed that the denitrification pathway of Az19 is incomplete, which was in agreement with a reduced growth on nitrate under low O2 concentrations. Putative genes of the general stress response and oxidative stress-tolerance, as well as synthesis of exopolysaccharides, carotenoids, polyamines and several osmolytes, were detected. An additional poly-beta-hydroxybutyrate (PHB) synthase coding gene was found in Az19 genome, but the accumulation of PHB did not increase under salinity. The detection of exclusive genes related to DNA repair led to discover that strain Az19 also has improved UV-tolerance, both in vitro and in planta. Finally, the analysis revealed the presence of multiple kaiC-like genes, which could be involved in stress-tolerance and, possibly, light responsiveness. Although A. brasilense has been a model for the study of beneficial plant-associated rhizobacteria, the evidence collected in this current study suggests, for the first time in this bacterial group, an unexpected possibility of adaptation to the phyllosphere. |
| format | Artículo |
| id | INTA13916 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA139162023-02-14T10:23:21Z Signs of a phyllospheric lifestyle in the genome of the stress-tolerant strain Azospirillum brasilense Az19 Garcia, Julia Elena Labarthe, María Mercedes Pagnussat, Luciana Anabella Amenta, Melina Beatriz Creus, Cecilia Mónica Maroniche, Guillermo Andrés Phyllosphere Azospirillum Brasilense Ultraviolet Radiation Phylogenetic Analysis Strains Radiación Ultravioleta Filosfera Análisis Filogenético Cepas Phylogenomics Incomplete Denitrification PHB UV-tolerance kaiC Strain Az19 Cepa Az19 Azospirillum brasilense Az19 is a plant-beneficial bacterium capable of protecting plants from the negative effects of drought. The objective of this study was to determine and analyze the genomic sequence of strain Az19 as a means of identifying putative stress-adaptation mechanisms. A high-quality draft genome of ca. 7 Mb with a predicted coding potential of 6710 genes was obtained. Phylogenomic analyses confirmed that Az19 belongs to the brasilense clade and is closely related to strains Az39 and REC3. Functional genomics revealed that the denitrification pathway of Az19 is incomplete, which was in agreement with a reduced growth on nitrate under low O2 concentrations. Putative genes of the general stress response and oxidative stress-tolerance, as well as synthesis of exopolysaccharides, carotenoids, polyamines and several osmolytes, were detected. An additional poly-beta-hydroxybutyrate (PHB) synthase coding gene was found in Az19 genome, but the accumulation of PHB did not increase under salinity. The detection of exclusive genes related to DNA repair led to discover that strain Az19 also has improved UV-tolerance, both in vitro and in planta. Finally, the analysis revealed the presence of multiple kaiC-like genes, which could be involved in stress-tolerance and, possibly, light responsiveness. Although A. brasilense has been a model for the study of beneficial plant-associated rhizobacteria, the evidence collected in this current study suggests, for the first time in this bacterial group, an unexpected possibility of adaptation to the phyllosphere. Instituto de Microbiología y Zoología Agrícola (IMYZA) Fil: Garcia, Julia Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Labarthe, María Mercedes. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina Fil: Pagnussat, Luciana Anabella. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina Fil: Pagnussat, Luciana Anabella. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Amenta, Melina Beatriz. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina Fil: Creus, Cecilia Mónica. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina Fil: Maroniche, Guillermo Andrés. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina Fil: Maroniche, Guillermo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2023-01-17T16:21:29Z 2023-01-17T16:21:29Z 2020-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/13916 https://www.sciencedirect.com/science/article/abs/pii/S0723202020300850 Systematic and applied microbiology 43 (6) : 126130. (Noviembre 2020) 0723-2020 1618-0984 https://doi.org/10.1016/j.syapm.2020.126130 eng info:eu-repograntAgreement/INTA/2019-PD-E4-I069-001/2019-PD-E4-I069-001/AR./Bioprospección y caracterización de microorganismos benéficos para la protección y producción vegetal info:eu-repograntAgreement/INTA/2019-PD-E2-I037-002/2019-PD-E2-I037-002/AR./Biodiversidad edáfica: componente clave para una gestión integral y sustentable del recurso suelo info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Systematic and applied microbiology 43 (6) : 126130. (Noviembre 2020) |
| spellingShingle | Phyllosphere Azospirillum Brasilense Ultraviolet Radiation Phylogenetic Analysis Strains Radiación Ultravioleta Filosfera Análisis Filogenético Cepas Phylogenomics Incomplete Denitrification PHB UV-tolerance kaiC Strain Az19 Cepa Az19 Garcia, Julia Elena Labarthe, María Mercedes Pagnussat, Luciana Anabella Amenta, Melina Beatriz Creus, Cecilia Mónica Maroniche, Guillermo Andrés Signs of a phyllospheric lifestyle in the genome of the stress-tolerant strain Azospirillum brasilense Az19 |
| title | Signs of a phyllospheric lifestyle in the genome of the stress-tolerant strain Azospirillum brasilense Az19 |
| title_full | Signs of a phyllospheric lifestyle in the genome of the stress-tolerant strain Azospirillum brasilense Az19 |
| title_fullStr | Signs of a phyllospheric lifestyle in the genome of the stress-tolerant strain Azospirillum brasilense Az19 |
| title_full_unstemmed | Signs of a phyllospheric lifestyle in the genome of the stress-tolerant strain Azospirillum brasilense Az19 |
| title_short | Signs of a phyllospheric lifestyle in the genome of the stress-tolerant strain Azospirillum brasilense Az19 |
| title_sort | signs of a phyllospheric lifestyle in the genome of the stress tolerant strain azospirillum brasilense az19 |
| topic | Phyllosphere Azospirillum Brasilense Ultraviolet Radiation Phylogenetic Analysis Strains Radiación Ultravioleta Filosfera Análisis Filogenético Cepas Phylogenomics Incomplete Denitrification PHB UV-tolerance kaiC Strain Az19 Cepa Az19 |
| url | http://hdl.handle.net/20.500.12123/13916 https://www.sciencedirect.com/science/article/abs/pii/S0723202020300850 https://doi.org/10.1016/j.syapm.2020.126130 |
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