Brucella canis Group 2 isolated in Argentina = Brucella canis Grupo 2 aislado en Argentina
The aim of this study was to estimate the diversity and prevalence of both groups of Brucella canis 1 and 2 with and without deletion respectively in different areas of Argentina. A total of 104 bacterial cultures were typed as B. canis strains using the classical biotyping method. Two PCR assays we...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Elsevier
2023
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| Online Access: | http://hdl.handle.net/20.500.12123/13910 https://www.sciencedirect.com/science/article/pii/S0325754120300687 https://doi.org/10.1016/j.ram.2020.06.012 |
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| author | Boeri, Eduardo Jorge Madariaga, María Julia Dominguez, María Luz Teijeiro, María Luisa Fernandez, Natalia Mercedes Elena, Sebastián Alejandro Trangoni, Marcos David |
| author_browse | Boeri, Eduardo Jorge Dominguez, María Luz Elena, Sebastián Alejandro Fernandez, Natalia Mercedes Madariaga, María Julia Teijeiro, María Luisa Trangoni, Marcos David |
| author_facet | Boeri, Eduardo Jorge Madariaga, María Julia Dominguez, María Luz Teijeiro, María Luisa Fernandez, Natalia Mercedes Elena, Sebastián Alejandro Trangoni, Marcos David |
| author_sort | Boeri, Eduardo Jorge |
| collection | INTA Digital |
| description | The aim of this study was to estimate the diversity and prevalence of both groups of Brucella canis 1 and 2 with and without deletion respectively in different areas of Argentina. A total of 104 bacterial cultures were typed as B. canis strains using the classical biotyping method. Two PCR assays were performed to confirm that all isolates were B. canis and not Brucella suis. The differentiation between groups 1 and 2 was achieved using another PCR assay and the diversity of B. canis isolates was assessed with four MLVA_16 markers. All strains belonged to Group 2. Bruce 09 marker (MLVA_16 assay) showed the greatest diversity. Only Group 2 of B. canis was identified among the strains evaluated. The markers chosen from the MLVA_16 allowed us to detect genetic diversity among the strains of B. canis studied. |
| format | info:ar-repo/semantics/artículo |
| id | INTA13910 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA139102023-01-13T15:40:14Z Brucella canis Group 2 isolated in Argentina = Brucella canis Grupo 2 aislado en Argentina Boeri, Eduardo Jorge Madariaga, María Julia Dominguez, María Luz Teijeiro, María Luisa Fernandez, Natalia Mercedes Elena, Sebastián Alejandro Trangoni, Marcos David Brucella canis Marcadores Genéticos Diversidad Genética (como Recurso) Genetic Markers Genetic Diversity (as Resource) The aim of this study was to estimate the diversity and prevalence of both groups of Brucella canis 1 and 2 with and without deletion respectively in different areas of Argentina. A total of 104 bacterial cultures were typed as B. canis strains using the classical biotyping method. Two PCR assays were performed to confirm that all isolates were B. canis and not Brucella suis. The differentiation between groups 1 and 2 was achieved using another PCR assay and the diversity of B. canis isolates was assessed with four MLVA_16 markers. All strains belonged to Group 2. Bruce 09 marker (MLVA_16 assay) showed the greatest diversity. Only Group 2 of B. canis was identified among the strains evaluated. The markers chosen from the MLVA_16 allowed us to detect genetic diversity among the strains of B. canis studied. El objetivo de este trabajo fue estimar la diversidad y la prevalencia de ambos grupos de Brucella canis 1 y 2 (con y sin deleción, respectivamente) en diferentes áreas de Argentina. Un total de 104 cultivos bacterianos se tipificaron como cepas de B. canis usando biotipado clásico. Se realizaron dos ensayos de PCR para confirmar que todos los aislamientos eran B. canis y no Brucella suis. La diferenciación entre los grupos 1 y 2 se logró con otro ensayo de PCR, y la diversidad entre las cepas de B. canis se obtuvo mediante el empleo de cuatro marcadores del ensayo de MLVA_16. Todas las cepas pertenecieron al grupo 2. El marcador Bruce 09 (ensayo MVLA-16) mostró la mayor diversidad. Sólo se halló el Grupo 2 de B. canis entre las cepas estudiadas. Los marcadores del MLVA_16 permitieron detectar la presencia de diversidad genética entre las cepas de B. canis analizadas. Instituto de Biotecnología Fil: Boeri, Eduardo Jorge. Instituto de Zoonosis Luis Pasteur; Argentina Fil: Madariaga, María Julia. Instituto de Zoonosis Luis Pasteur; Argentina Fil: Dominguez, María Luz. Instituto de Zoonosis Luis Pasteur; Argentina Fil: Teijeiro, María Luisa. Instituto de Zoonosis Luis Pasteur; Argentina Fil: Fernandez, Natalia Mercedes. Instituto de Zoonosis Luis Pasteur; Argentina Fil: Elena, Sebastián. Servicio Nacional de Sanidad y Calidad Agroalimentaria. Dirección General de Laboratorio y Control Técnico. Laboratorio de Referencia de la OIE para Brucelosis; Argentina Fil: Trangoni, Marcos David. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular. Argentina Fil: Trangoni, Marcos David. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2023-01-13T15:23:21Z 2023-01-13T15:23:21Z 2021-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/13910 https://www.sciencedirect.com/science/article/pii/S0325754120300687 0325-7541 1851-7617 https://doi.org/10.1016/j.ram.2020.06.012 eng info:eu-repo/semantics/openAccess application/pdf Elsevier Revista Argentina de Microbiología 53 (2) : 98-103 (April–June 2021) |
| spellingShingle | Brucella canis Marcadores Genéticos Diversidad Genética (como Recurso) Genetic Markers Genetic Diversity (as Resource) Boeri, Eduardo Jorge Madariaga, María Julia Dominguez, María Luz Teijeiro, María Luisa Fernandez, Natalia Mercedes Elena, Sebastián Alejandro Trangoni, Marcos David Brucella canis Group 2 isolated in Argentina = Brucella canis Grupo 2 aislado en Argentina |
| title | Brucella canis Group 2 isolated in Argentina = Brucella canis Grupo 2 aislado en Argentina |
| title_full | Brucella canis Group 2 isolated in Argentina = Brucella canis Grupo 2 aislado en Argentina |
| title_fullStr | Brucella canis Group 2 isolated in Argentina = Brucella canis Grupo 2 aislado en Argentina |
| title_full_unstemmed | Brucella canis Group 2 isolated in Argentina = Brucella canis Grupo 2 aislado en Argentina |
| title_short | Brucella canis Group 2 isolated in Argentina = Brucella canis Grupo 2 aislado en Argentina |
| title_sort | brucella canis group 2 isolated in argentina brucella canis grupo 2 aislado en argentina |
| topic | Brucella canis Marcadores Genéticos Diversidad Genética (como Recurso) Genetic Markers Genetic Diversity (as Resource) |
| url | http://hdl.handle.net/20.500.12123/13910 https://www.sciencedirect.com/science/article/pii/S0325754120300687 https://doi.org/10.1016/j.ram.2020.06.012 |
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