Efficacy of chestnut and quebracho wood extracts to control Salmonella in poultry

Aims: The study was aimed to evaluate the antibacterial activity and efficacy of chestnut and quebracho wood extracts against Salmonella by in vitro assays and in vivo trials. Methods and results: The extracts showed inhibitory activity against Salmonella determined by the minimum inhibitory concen...

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Autores principales: Casanova, Natalia Andrea, Redondo, Leandro Martin, Redondo, Enzo Alejandro, Joaquim, Patricia Estefania, Dominguez, Johana Natali, Fernandez Miyakawa, Mariano Enrique, Chacana, Pablo
Formato: Artículo
Lenguaje:Inglés
Publicado: Wiley 2023
Materias:
Acceso en línea:http://hdl.handle.net/20.500.12123/13908
https://ami-journals.onlinelibrary.wiley.com/doi/10.1111/jam.14948
https://doi.org/10.1111/jam.14948
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author Casanova, Natalia Andrea
Redondo, Leandro Martin
Redondo, Enzo Alejandro
Joaquim, Patricia Estefania
Dominguez, Johana Natali
Fernandez Miyakawa, Mariano Enrique
Chacana, Pablo
author_browse Casanova, Natalia Andrea
Chacana, Pablo
Dominguez, Johana Natali
Fernandez Miyakawa, Mariano Enrique
Joaquim, Patricia Estefania
Redondo, Enzo Alejandro
Redondo, Leandro Martin
author_facet Casanova, Natalia Andrea
Redondo, Leandro Martin
Redondo, Enzo Alejandro
Joaquim, Patricia Estefania
Dominguez, Johana Natali
Fernandez Miyakawa, Mariano Enrique
Chacana, Pablo
author_sort Casanova, Natalia Andrea
collection INTA Digital
description Aims: The study was aimed to evaluate the antibacterial activity and efficacy of chestnut and quebracho wood extracts against Salmonella by in vitro assays and in vivo trials. Methods and results: The extracts showed inhibitory activity against Salmonella determined by the minimum inhibitory concentration method as well as on the adhesion and invasion of S. Gallinarum (SG) and S. Enteritidis (SE) in Caco-2 cells. Also, transmission electron microscopy revealed that extract-treated Salmonella showed disruption of cell walls and membranes, damage of the cytoplasm and tannin-protein aggregations. In addition, efficacy of the extracts to control SG and SE was evaluated in experimental infection trials in laying hens and broilers respectively. SE excretion was significantly reduced on days 5 (P < 0·01) and 12 (P < 0·025) only in the quebracho group. In the fowl typhoid infection model, hens that received the chestnut extract showed a significantly reduced mortality (P < 0·05). Conclusions: Our results evidence that these alternative natural products may be a useful tool to control Salmonella in poultry.
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spelling INTA139082024-07-31T14:16:57Z Efficacy of chestnut and quebracho wood extracts to control Salmonella in poultry Casanova, Natalia Andrea Redondo, Leandro Martin Redondo, Enzo Alejandro Joaquim, Patricia Estefania Dominguez, Johana Natali Fernandez Miyakawa, Mariano Enrique Chacana, Pablo Aves de Corral Castaña Madera Control de Enfermedades Salmonella Poultry Chestnuts Wood Diseases Control Quebracho Schinopsis lorentzii Aims: The study was aimed to evaluate the antibacterial activity and efficacy of chestnut and quebracho wood extracts against Salmonella by in vitro assays and in vivo trials. Methods and results: The extracts showed inhibitory activity against Salmonella determined by the minimum inhibitory concentration method as well as on the adhesion and invasion of S. Gallinarum (SG) and S. Enteritidis (SE) in Caco-2 cells. Also, transmission electron microscopy revealed that extract-treated Salmonella showed disruption of cell walls and membranes, damage of the cytoplasm and tannin-protein aggregations. In addition, efficacy of the extracts to control SG and SE was evaluated in experimental infection trials in laying hens and broilers respectively. SE excretion was significantly reduced on days 5 (P < 0·01) and 12 (P < 0·025) only in the quebracho group. In the fowl typhoid infection model, hens that received the chestnut extract showed a significantly reduced mortality (P < 0·05). Conclusions: Our results evidence that these alternative natural products may be a useful tool to control Salmonella in poultry. Instituto de Patobiología Fil: Casanova, Natalia Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Redondo, Leandro Martí­n. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Redondo, Leandro Martí­n. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Redondo, Enzo Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Redondo, Enzo Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Joaquim, Patricia Estefania. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Dominguez, Johana Natali. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Dominguez, Johana Natali. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Fernandez Miyakawa, Mariano Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Fernandez Miyakawa, Mariano Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Chacana, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina 2023-01-13T14:30:01Z 2023-01-13T14:30:01Z 2021-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/13908 https://ami-journals.onlinelibrary.wiley.com/doi/10.1111/jam.14948 1364-5072 1365-2672 https://doi.org/10.1111/jam.14948 eng info:eu-repo/semantics/openAccess application/pdf Wiley Journal of Applied Microbiology 131 (1) : 135-145. (July 2021)
spellingShingle Aves de Corral
Castaña
Madera
Control de Enfermedades
Salmonella
Poultry
Chestnuts
Wood
Diseases Control
Quebracho
Schinopsis lorentzii
Casanova, Natalia Andrea
Redondo, Leandro Martin
Redondo, Enzo Alejandro
Joaquim, Patricia Estefania
Dominguez, Johana Natali
Fernandez Miyakawa, Mariano Enrique
Chacana, Pablo
Efficacy of chestnut and quebracho wood extracts to control Salmonella in poultry
title Efficacy of chestnut and quebracho wood extracts to control Salmonella in poultry
title_full Efficacy of chestnut and quebracho wood extracts to control Salmonella in poultry
title_fullStr Efficacy of chestnut and quebracho wood extracts to control Salmonella in poultry
title_full_unstemmed Efficacy of chestnut and quebracho wood extracts to control Salmonella in poultry
title_short Efficacy of chestnut and quebracho wood extracts to control Salmonella in poultry
title_sort efficacy of chestnut and quebracho wood extracts to control salmonella in poultry
topic Aves de Corral
Castaña
Madera
Control de Enfermedades
Salmonella
Poultry
Chestnuts
Wood
Diseases Control
Quebracho
Schinopsis lorentzii
url http://hdl.handle.net/20.500.12123/13908
https://ami-journals.onlinelibrary.wiley.com/doi/10.1111/jam.14948
https://doi.org/10.1111/jam.14948
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