A novel MHC-II targeted BVDV subunit vaccine induces a neutralizing immunological response in guinea pigs and cattle
Bovine viral diarrhoea virus (BVDV) is a major cause of economic loss in the cattle industry, worldwide. Infection results in reduced productive performance, growth retardation, reduced milk production and increased susceptibility to other diseases leading to early culling of animals. There are two...
| Autores principales: | , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2023
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/13907 https://onlinelibrary.wiley.com/doi/abs/10.1111/tbed.13952 https://doi.org/10.1111/tbed.13952 |
| _version_ | 1855485262590640128 |
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| author | Bellido, Demian Baztarrica, Josefina Rocha, Lucía Alejandra Pecora, Andrea Acosta, Mario Escribano, José M. Parreño, Gladys Wigdorovitz, Andres |
| author_browse | Acosta, Mario Baztarrica, Josefina Bellido, Demian Escribano, José M. Parreño, Gladys Pecora, Andrea Rocha, Lucía Alejandra Wigdorovitz, Andres |
| author_facet | Bellido, Demian Baztarrica, Josefina Rocha, Lucía Alejandra Pecora, Andrea Acosta, Mario Escribano, José M. Parreño, Gladys Wigdorovitz, Andres |
| author_sort | Bellido, Demian |
| collection | INTA Digital |
| description | Bovine viral diarrhoea virus (BVDV) is a major cause of economic loss in the cattle industry, worldwide. Infection results in reduced productive performance, growth retardation, reduced milk production and increased susceptibility to other diseases leading to early culling of animals. There are two primary methods used to control the spread of BVDV: the elimination of persistently infected (PI) animals and vaccination. Currently, modified live or inactivated vaccines are used in BVDV vaccination programmes, but there are safety risks or insufficient protection, respectively, with these vaccines. Here, we report the development and efficacy of the first targeted subunit vaccine against BVDV. The core of the vaccine is the fusion of the BVDV structural protein, E2, to a single-chain antibody, APCH, together termed, APCH-E2. The APCH antibody targets the E2 antigen to the major histocompatibility type II molecule (MHC-II) present on antigen-presenting cells. Industrial production of the vaccine is carried out using the baculovirus expression vector system (BEVS) using single-use manufacturing technologies. This new subunit vaccine induces strong BVDV-specific neutralizing antibodies in guinea pigs and cattle. Importantly, in cattle with low levels of natural BVDV-specific neutralizing antibodies, the vaccine induced strong neutralizing antibody levels to above the protective threshold, as determined by a competition ELISA. The APCH-E2 vaccine induced a rapid and sustained neutralizing antibody response compared with a conventional vaccine in cattle. |
| format | Artículo |
| id | INTA13907 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA139072023-01-13T13:52:18Z A novel MHC-II targeted BVDV subunit vaccine induces a neutralizing immunological response in guinea pigs and cattle Bellido, Demian Baztarrica, Josefina Rocha, Lucía Alejandra Pecora, Andrea Acosta, Mario Escribano, José M. Parreño, Gladys Wigdorovitz, Andres Pestivirus de la Diarrea Bovina Vacuna Respuesta Inmunológica Ganado Bovino Cobaya Bovine Diarrhoea Pestivirus Vaccines Immune Response Cattle Guinea Pigs BVDV Bovine viral diarrhoea virus (BVDV) is a major cause of economic loss in the cattle industry, worldwide. Infection results in reduced productive performance, growth retardation, reduced milk production and increased susceptibility to other diseases leading to early culling of animals. There are two primary methods used to control the spread of BVDV: the elimination of persistently infected (PI) animals and vaccination. Currently, modified live or inactivated vaccines are used in BVDV vaccination programmes, but there are safety risks or insufficient protection, respectively, with these vaccines. Here, we report the development and efficacy of the first targeted subunit vaccine against BVDV. The core of the vaccine is the fusion of the BVDV structural protein, E2, to a single-chain antibody, APCH, together termed, APCH-E2. The APCH antibody targets the E2 antigen to the major histocompatibility type II molecule (MHC-II) present on antigen-presenting cells. Industrial production of the vaccine is carried out using the baculovirus expression vector system (BEVS) using single-use manufacturing technologies. This new subunit vaccine induces strong BVDV-specific neutralizing antibodies in guinea pigs and cattle. Importantly, in cattle with low levels of natural BVDV-specific neutralizing antibodies, the vaccine induced strong neutralizing antibody levels to above the protective threshold, as determined by a competition ELISA. The APCH-E2 vaccine induced a rapid and sustained neutralizing antibody response compared with a conventional vaccine in cattle. Instituto de Virologia Fil: Bellido, Demian. Vetanco SA; Argentina Fil: Bellido, Demian. Bioinnovo SA; Argentina Fil: Baztarrica, Josefina. Vetanco SA; Argentina Fil: Baztarrica, Josefina. Bioinnovo SA; Argentina Fil: Rocha, Lucía Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Incuinta e Instituto de Virología; Argentina Fil: Rocha, Lucía Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Pecora, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Incuinta e Instituto de Virología; Argentina Fil: Pecora, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Acosta, Mario. Vetanco SA; Argentina Fil: Escribano, José M. Algenex; España Fil: Parreño, Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Incuinta e Instituto de Virología; Argentina Fil: Parreño, Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Parreño, Viviana. Bioinnovo S.A.; Argentina Fil: Wigdorovitz, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Incuinta e Instituto de Virología; Argentina Fil: Wigdorovitz, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Wigdorovitz, Andres. Bioinnovo S.A.; Argentina 2023-01-13T13:50:39Z 2023-01-13T13:50:39Z 2021-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/13907 https://onlinelibrary.wiley.com/doi/abs/10.1111/tbed.13952 1865-1674 1865-1682 https://doi.org/10.1111/tbed.13952 eng info:eu-repo/semantics/openAccess application/pdf Wiley Transboundary and Emerging Diseases 68 (6) : 3474-3481 (November 2021) |
| spellingShingle | Pestivirus de la Diarrea Bovina Vacuna Respuesta Inmunológica Ganado Bovino Cobaya Bovine Diarrhoea Pestivirus Vaccines Immune Response Cattle Guinea Pigs BVDV Bellido, Demian Baztarrica, Josefina Rocha, Lucía Alejandra Pecora, Andrea Acosta, Mario Escribano, José M. Parreño, Gladys Wigdorovitz, Andres A novel MHC-II targeted BVDV subunit vaccine induces a neutralizing immunological response in guinea pigs and cattle |
| title | A novel MHC-II targeted BVDV subunit vaccine induces a neutralizing immunological response in guinea pigs and cattle |
| title_full | A novel MHC-II targeted BVDV subunit vaccine induces a neutralizing immunological response in guinea pigs and cattle |
| title_fullStr | A novel MHC-II targeted BVDV subunit vaccine induces a neutralizing immunological response in guinea pigs and cattle |
| title_full_unstemmed | A novel MHC-II targeted BVDV subunit vaccine induces a neutralizing immunological response in guinea pigs and cattle |
| title_short | A novel MHC-II targeted BVDV subunit vaccine induces a neutralizing immunological response in guinea pigs and cattle |
| title_sort | novel mhc ii targeted bvdv subunit vaccine induces a neutralizing immunological response in guinea pigs and cattle |
| topic | Pestivirus de la Diarrea Bovina Vacuna Respuesta Inmunológica Ganado Bovino Cobaya Bovine Diarrhoea Pestivirus Vaccines Immune Response Cattle Guinea Pigs BVDV |
| url | http://hdl.handle.net/20.500.12123/13907 https://onlinelibrary.wiley.com/doi/abs/10.1111/tbed.13952 https://doi.org/10.1111/tbed.13952 |
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