Dissecting light sensing and metabolic pathways on the millimeter scale in high-altitude modern stromatolites
Modern non-lithifying stromatolites on the shore of the volcanic lake Socompa (SST) in the Puna are affected by several extreme conditions. The present study assesses for the first time light utilization and functional metabolic stratification of SST on a millimeter scale through shotgun metagenomic...
| Autores principales: | , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2023
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/13899 https://link.springer.com/article/10.1007/s00248-022-02112-7 https://doi.org/10.1007/s00248-022-02112-7 |
| _version_ | 1855037021659070464 |
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| author | Alonso-Reyes, Daniel Gonzalo Galván, Fátima Silvina Irazoqui, Jose Matias Amadio, Ariel Tschoeke, Diogo Thompson, Fabiano Albarracín, Virginia Helena Farias, María Eugenia |
| author_browse | Albarracín, Virginia Helena Alonso-Reyes, Daniel Gonzalo Amadio, Ariel Farias, María Eugenia Galván, Fátima Silvina Irazoqui, Jose Matias Thompson, Fabiano Tschoeke, Diogo |
| author_facet | Alonso-Reyes, Daniel Gonzalo Galván, Fátima Silvina Irazoqui, Jose Matias Amadio, Ariel Tschoeke, Diogo Thompson, Fabiano Albarracín, Virginia Helena Farias, María Eugenia |
| author_sort | Alonso-Reyes, Daniel Gonzalo |
| collection | INTA Digital |
| description | Modern non-lithifying stromatolites on the shore of the volcanic lake Socompa (SST) in the Puna are affected by several extreme conditions. The present study assesses for the first time light utilization and functional metabolic stratification of SST on a millimeter scale through shotgun metagenomics. In addition, a scanning-electron-microscopy approach was used to explore the community. The analysis on SST unveiled the profile of a photosynthetic mat, with cyanobacteria not directly exposed to light, but placed just below a high-UV-resistant community. Calvin–Benson and 3-hydroxypropinate cycles for carbon fixation were abundant in upper, oxic layers, while the Wood–Ljungdahl pathway was dominant in the deeper anoxic strata. The high abundance of genes for UV-screening and oxidant-quenching pigments and CPF (photoreactivation) in the UV-stressed layers could indicate that the zone itself works as a UV shield. There is a remarkable density of sequences associated with photoreceptors in the first two layers. Also, genetic evidence of photosynthesis split in eukaryotic (layer 1) and prokaryotic (layer 2). Photoheterotrophic bacteria, aerobic photoautotrophic bacteria, and anaerobic photoautotrophic bacteria coexist by selectively absorbing different parts of the light spectrum (blue, red, and IR respectively) at different positions of the mat. Genes for oxygen, nitrogen, and sulfur metabolism account for the microelectrode chemical data and pigment measurements performed in previous publications. We also provide here an explanation for the vertical microbial mobility within the SST described previously. Finally, our study points to SST as ideal modern analogues of ancient ST. |
| format | info:ar-repo/semantics/artículo |
| id | INTA13899 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA138992023-01-12T16:26:38Z Dissecting light sensing and metabolic pathways on the millimeter scale in high-altitude modern stromatolites Alonso-Reyes, Daniel Gonzalo Galván, Fátima Silvina Irazoqui, Jose Matias Amadio, Ariel Tschoeke, Diogo Thompson, Fabiano Albarracín, Virginia Helena Farias, María Eugenia Stromatolites Microbiomes Ultraviolet Radiation Estromatolitos Microbiomas Radiación Ultravioleta Metagenomics Metagenómica Modern non-lithifying stromatolites on the shore of the volcanic lake Socompa (SST) in the Puna are affected by several extreme conditions. The present study assesses for the first time light utilization and functional metabolic stratification of SST on a millimeter scale through shotgun metagenomics. In addition, a scanning-electron-microscopy approach was used to explore the community. The analysis on SST unveiled the profile of a photosynthetic mat, with cyanobacteria not directly exposed to light, but placed just below a high-UV-resistant community. Calvin–Benson and 3-hydroxypropinate cycles for carbon fixation were abundant in upper, oxic layers, while the Wood–Ljungdahl pathway was dominant in the deeper anoxic strata. The high abundance of genes for UV-screening and oxidant-quenching pigments and CPF (photoreactivation) in the UV-stressed layers could indicate that the zone itself works as a UV shield. There is a remarkable density of sequences associated with photoreceptors in the first two layers. Also, genetic evidence of photosynthesis split in eukaryotic (layer 1) and prokaryotic (layer 2). Photoheterotrophic bacteria, aerobic photoautotrophic bacteria, and anaerobic photoautotrophic bacteria coexist by selectively absorbing different parts of the light spectrum (blue, red, and IR respectively) at different positions of the mat. Genes for oxygen, nitrogen, and sulfur metabolism account for the microelectrode chemical data and pigment measurements performed in previous publications. We also provide here an explanation for the vertical microbial mobility within the SST described previously. Finally, our study points to SST as ideal modern analogues of ancient ST. EEA Rafaela Fil: Alonso-Reyes, Daniel Gonzalo. Universidad Nacional de Tucumán. Centro Integral de Microscopía Electrónica (CIME). Laboratorio de Microbiología Ultraestructural y Molecular; Argentina Fil: Alonso-Reyes, Daniel Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Integral de Microscopía Electrónica (CIME). Laboratorio de Microbiología Ultraestructural y Molecular; Argentina Fil: Alonso-Reyes, Daniel Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas. Planta Piloto de Procesos Industriales y Microbiológicos (PROIMI). Laboratorio de Investigaciones Microbiológicas de Lagunas Andinas; Argentina Fil: Galván, Fátima Silvina. Universidad Nacional de Tucumán. Centro Integral de Microscopía Electrónica. Laboratorio de Microbiología Ultraestructural y Molecular; Argentina Fil: Galván, Fátima Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Integral de Microscopía Electrónica. Laboratorio de Microbiología Ultraestructural y Molecular; Argentina Fil: Irazoqui, Jose Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea; Argentina Fil: Irazoqui, Jose Matias. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina Fil: Amadio, Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina Fil: Amadio, Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea; Argentina Fil: Diogo Tschoeke. Federal University of Rio de Janeiro. Institute of Biology and Coppe; Brasil Fil: Fabiano Thompson. Federal University of Rio de Janeiro. Institute of Biology and Coppe; Brasil Fil: Albarracín, Virginia Helena. Universidad Nacional de Tucumán. Centro Integral de Microscopía Electrónica (CIME). Laboratorio de Microbiología Ultraestructural y Molecular; Argentina Fil: Albarracín, Virginia Helena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Integral de Microscopía Electrónica (CIME). Laboratorio de Microbiología Ultraestructural y Molecular; Argentina Fil: Albarracín, Virginia Helena. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina Fil: Farias, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Planta Piloto de Procesos Industriales y Microbiológicos (PROIMI). Laboratorio de Investigaciones Microbiológicas de Lagunas Andinas; Argentina 2023-01-12T16:12:33Z 2023-01-12T16:12:33Z 2022-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/13899 https://link.springer.com/article/10.1007/s00248-022-02112-7 Alonso-Reyes, D.G., Galván, F., Irazoqui, J.M. et al. Dissecting Light Sensing and Metabolic Pathways on the Millimeter Scale in High-Altitude Modern Stromatolites. Microb Ecol (2022). https://doi.org/10.1007/s00248-022-02112-7 0095-3628 https://doi.org/10.1007/s00248-022-02112-7 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Microbial Ecology (Published: 26 September 2022) |
| spellingShingle | Stromatolites Microbiomes Ultraviolet Radiation Estromatolitos Microbiomas Radiación Ultravioleta Metagenomics Metagenómica Alonso-Reyes, Daniel Gonzalo Galván, Fátima Silvina Irazoqui, Jose Matias Amadio, Ariel Tschoeke, Diogo Thompson, Fabiano Albarracín, Virginia Helena Farias, María Eugenia Dissecting light sensing and metabolic pathways on the millimeter scale in high-altitude modern stromatolites |
| title | Dissecting light sensing and metabolic pathways on the millimeter scale in high-altitude modern stromatolites |
| title_full | Dissecting light sensing and metabolic pathways on the millimeter scale in high-altitude modern stromatolites |
| title_fullStr | Dissecting light sensing and metabolic pathways on the millimeter scale in high-altitude modern stromatolites |
| title_full_unstemmed | Dissecting light sensing and metabolic pathways on the millimeter scale in high-altitude modern stromatolites |
| title_short | Dissecting light sensing and metabolic pathways on the millimeter scale in high-altitude modern stromatolites |
| title_sort | dissecting light sensing and metabolic pathways on the millimeter scale in high altitude modern stromatolites |
| topic | Stromatolites Microbiomes Ultraviolet Radiation Estromatolitos Microbiomas Radiación Ultravioleta Metagenomics Metagenómica |
| url | http://hdl.handle.net/20.500.12123/13899 https://link.springer.com/article/10.1007/s00248-022-02112-7 https://doi.org/10.1007/s00248-022-02112-7 |
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