Genome-based reclassification of Azospirillum brasilense Az39 as the type strain of Azospirillum argentinense sp. nov.
Strain Az39T of Azospirillum is a diazotrophic plant growth-promoting bacterium isolated in 1982 from the roots of wheat plants growing in Marcos Juárez, Córdoba, Argentina. It produces indole-3- acetic acid in the presence of l-tryptophan as a precursor, grows at 20–38 °C (optimal 38 °C), and the c...
| Autores principales: | , , , , , , , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Microbiology Society
2023
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/13898 https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.005475 https://doi.org/10.1099/ijsem.0.005475 |
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| author | dos Santos Ferreira, Natália Coniglio, Nayla Anahí Puente, Mariana Laura Sant’Anna, Fernando Hayashi Maroniche, Guillermo Andrés Garcia, Julia Elena Molina, Romina Micaela Nievas, Sofía Volpiano, Camila Gazolla Ambrosini, Adriana Passaglia, Luciane Maria Pereira Pedraza, Raúl Osvaldo Reis, Veronica Massena Zilli, Jerri Edson Cassan, Fabricio Dario |
| author_browse | Ambrosini, Adriana Cassan, Fabricio Dario Coniglio, Nayla Anahí Garcia, Julia Elena Maroniche, Guillermo Andrés Molina, Romina Micaela Nievas, Sofía Passaglia, Luciane Maria Pereira Pedraza, Raúl Osvaldo Puente, Mariana Laura Reis, Veronica Massena Sant’Anna, Fernando Hayashi Volpiano, Camila Gazolla Zilli, Jerri Edson dos Santos Ferreira, Natália |
| author_facet | dos Santos Ferreira, Natália Coniglio, Nayla Anahí Puente, Mariana Laura Sant’Anna, Fernando Hayashi Maroniche, Guillermo Andrés Garcia, Julia Elena Molina, Romina Micaela Nievas, Sofía Volpiano, Camila Gazolla Ambrosini, Adriana Passaglia, Luciane Maria Pereira Pedraza, Raúl Osvaldo Reis, Veronica Massena Zilli, Jerri Edson Cassan, Fabricio Dario |
| author_sort | dos Santos Ferreira, Natália |
| collection | INTA Digital |
| description | Strain Az39T of Azospirillum is a diazotrophic plant growth-promoting bacterium isolated in 1982 from the roots of wheat plants growing in Marcos Juárez, Córdoba, Argentina. It produces indole-3- acetic acid in the presence of l-tryptophan as a precursor, grows at 20–38 °C (optimal 38 °C), and the cells are curved or spiral-shaped, with diameters ranging from 0.5–0.9 to 1.8–2.2 μm. They contain C16 : 0, C18 : 0 and C18 : 1 ω7c/ω6c as the main fatty acids. Phylogenetic analysis of its 16S rRNA gene sequence confirmed that this strain belongs to the genus Azospirillum, showing a close relationship with Azospirillum baldaniorum Sp245T, Azospirillum brasilense Sp7T and Azospirillum formosense CC-Nfb- 7T. Housekeeping gene analysis revealed that Az39T, together with five strains of the genus (Az19, REC3, BR 11975, MTCC4035 and MTCC4036), form a cluster apart from A. baldaniorum Sp245T, A. brasilense Sp7T and A. formosense CC-Nfb- 7T. Average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) between Az39T and the aforementioned type strains revealed values below 96 %, the circumscription limit
for the species delineation (ANI: 95.3, 94.1 and 94.0 %; dDDH: 62.9, 56.3 and 55.6 %). Furthermore, a phylogeny evaluation of the core proteome, including 809 common shared proteins, showed an independent grouping of Az39T, Az19, REC3, BR 11975, MTCC4035 and MTCC4036. The G+C content in the genomic DNA of these six strains varied from 68.3 to 68.5 %. Based on the combined phylogenetic, genomic and phenotypic characterization presented here, we consider that strain Az39T, along with strains Az19, REC3, BR 11975, MTCC4035 and MTCC4036, are members of a new Azospirillum species, for which the name Azospirillum argentinense sp. nov. is proposed. The type strain is Az39T (=LBPCV39T=BR 148428T=CCCT 22.01T). |
| format | info:ar-repo/semantics/artículo |
| id | INTA13898 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Microbiology Society |
| publisherStr | Microbiology Society |
| record_format | dspace |
| spelling | INTA138982023-01-12T16:10:35Z Genome-based reclassification of Azospirillum brasilense Az39 as the type strain of Azospirillum argentinense sp. nov. dos Santos Ferreira, Natália Coniglio, Nayla Anahí Puente, Mariana Laura Sant’Anna, Fernando Hayashi Maroniche, Guillermo Andrés Garcia, Julia Elena Molina, Romina Micaela Nievas, Sofía Volpiano, Camila Gazolla Ambrosini, Adriana Passaglia, Luciane Maria Pereira Pedraza, Raúl Osvaldo Reis, Veronica Massena Zilli, Jerri Edson Cassan, Fabricio Dario Azospirillum Filogenia Genomes Azospirillum brasilense Phylogeny Genomas Azospirillum argentinense Strain Az39T of Azospirillum is a diazotrophic plant growth-promoting bacterium isolated in 1982 from the roots of wheat plants growing in Marcos Juárez, Córdoba, Argentina. It produces indole-3- acetic acid in the presence of l-tryptophan as a precursor, grows at 20–38 °C (optimal 38 °C), and the cells are curved or spiral-shaped, with diameters ranging from 0.5–0.9 to 1.8–2.2 μm. They contain C16 : 0, C18 : 0 and C18 : 1 ω7c/ω6c as the main fatty acids. Phylogenetic analysis of its 16S rRNA gene sequence confirmed that this strain belongs to the genus Azospirillum, showing a close relationship with Azospirillum baldaniorum Sp245T, Azospirillum brasilense Sp7T and Azospirillum formosense CC-Nfb- 7T. Housekeeping gene analysis revealed that Az39T, together with five strains of the genus (Az19, REC3, BR 11975, MTCC4035 and MTCC4036), form a cluster apart from A. baldaniorum Sp245T, A. brasilense Sp7T and A. formosense CC-Nfb- 7T. Average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) between Az39T and the aforementioned type strains revealed values below 96 %, the circumscription limit for the species delineation (ANI: 95.3, 94.1 and 94.0 %; dDDH: 62.9, 56.3 and 55.6 %). Furthermore, a phylogeny evaluation of the core proteome, including 809 common shared proteins, showed an independent grouping of Az39T, Az19, REC3, BR 11975, MTCC4035 and MTCC4036. The G+C content in the genomic DNA of these six strains varied from 68.3 to 68.5 %. Based on the combined phylogenetic, genomic and phenotypic characterization presented here, we consider that strain Az39T, along with strains Az19, REC3, BR 11975, MTCC4035 and MTCC4036, are members of a new Azospirillum species, for which the name Azospirillum argentinense sp. nov. is proposed. The type strain is Az39T (=LBPCV39T=BR 148428T=CCCT 22.01T). Instituto de Microbiología y Zoología Agrícola (IMYZA) Fil: Dos Santos Ferreira, Natália. Universidade Federal Rural Do Rio de Janeiro; Brasil Fil: Coniglio, Nayla Anahí. Universidad Nacional de Río Cuarto. Instituto de Investigaciones Agrobiotecnológicas (INIAB-CONICET). Laboratorio de Fisiología Vegetal y de la Interacción Planta-Microorganismo; Argentina Fil: Puente, Mariana Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola. Laboratorio de Bacterias Promotoras del Crecimiento Vegetal; Argentina Fil: Sant´Anna, Fernando Hayashi. PROADI-SUS. Hospital Moinhos de Vento; Brasil Fil: Maroniche, Guillermo Andrés. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina Fil: Maroniche, Guillermo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Garcia, Julia Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Molina, Romina Micaela. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones Agrobiotecnológicas. Laboratorio de Fisiología Vegetal e Interacción Planta-Microorganismo; Argentina Fil: Nievas, Sofía. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones Agrobiotecnológicas. Laboratorio de Fisiología Vegetal e Interacción Planta-Microorganismo; Argentina Fil: Volpiano, Camila Gazolla. Instituto de Biociências. Departamento de Genética and Programa de Pós-graduação em Genética e Biologia Molecular; Brasil Fil: Ambrosini, Adriana. Instituto de Biociências. Departamento de Genética and Programa de Pós-graduação em Genética e Biologia Molecular; Brasil Fil: Passaglia, Luciane Maria Pereira. Instituto de Biociências. Departamento de Genética and Programa de Pós-graduação em Genética e Biologia Molecular; Brasil Fil: Pedraza, Raúl Osvaldo. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina Fil: Reis, Veronica Massena. Empresa Brasileira de Pesquisa Agropecuaria (EMBRAPA). Agrobiologia; Brasil Fil: Zilli, Jerri Edson. Empresa Brasileira de Pesquisa Agropecuaria (EMBRAPA). Agrobiologia; Brasil Fil: Cassan, Fabricio Dario. Universidad Nacional de Río Cuarto. Instituto de Investigaciones Agrobiotecnológicas (INIAB-CONICET). Laboratorio de Fisiología Vegetal y de la Interacción Planta-Microorganismo; Argentina 2023-01-12T15:56:40Z 2023-01-12T15:56:40Z 2022-08-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/13898 https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.005475 1466-5026 https://doi.org/10.1099/ijsem.0.005475 eng info:eu-repo/semantics/restrictedAccess application/pdf Microbiology Society International Journal of Systematic and Evolutionary Microbiology 72 : 005475 (2022) |
| spellingShingle | Azospirillum Filogenia Genomes Azospirillum brasilense Phylogeny Genomas Azospirillum argentinense dos Santos Ferreira, Natália Coniglio, Nayla Anahí Puente, Mariana Laura Sant’Anna, Fernando Hayashi Maroniche, Guillermo Andrés Garcia, Julia Elena Molina, Romina Micaela Nievas, Sofía Volpiano, Camila Gazolla Ambrosini, Adriana Passaglia, Luciane Maria Pereira Pedraza, Raúl Osvaldo Reis, Veronica Massena Zilli, Jerri Edson Cassan, Fabricio Dario Genome-based reclassification of Azospirillum brasilense Az39 as the type strain of Azospirillum argentinense sp. nov. |
| title | Genome-based reclassification of Azospirillum brasilense Az39 as the type strain of Azospirillum argentinense sp. nov. |
| title_full | Genome-based reclassification of Azospirillum brasilense Az39 as the type strain of Azospirillum argentinense sp. nov. |
| title_fullStr | Genome-based reclassification of Azospirillum brasilense Az39 as the type strain of Azospirillum argentinense sp. nov. |
| title_full_unstemmed | Genome-based reclassification of Azospirillum brasilense Az39 as the type strain of Azospirillum argentinense sp. nov. |
| title_short | Genome-based reclassification of Azospirillum brasilense Az39 as the type strain of Azospirillum argentinense sp. nov. |
| title_sort | genome based reclassification of azospirillum brasilense az39 as the type strain of azospirillum argentinense sp nov |
| topic | Azospirillum Filogenia Genomes Azospirillum brasilense Phylogeny Genomas Azospirillum argentinense |
| url | http://hdl.handle.net/20.500.12123/13898 https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.005475 https://doi.org/10.1099/ijsem.0.005475 |
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