Differential immune response to two Staphylococcus aureus strains with distinct adaptation genotypes after experimental intramammary infection of dairy cows
The aim of this study was to evaluate and compare the ability of two S. aureus strains with different adaptation genotypes (low and high) to the bovine mammary gland (MG) to establish an intramammary infection (IMI) and induce an immune response after an experimental challenge in lactating cows. Two...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
| Published: |
Elsevier
2023
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/13892 https://www.sciencedirect.com/science/article/pii/S0882401022004028 https://doi.org/10.1016/j.micpath.2022.105789 |
| _version_ | 1855037020571697152 |
|---|---|
| author | Engler, Carolina Renna, María Sol Beccaria, Camila Silvestrini, Paula Pirola, Silvana I. Pereyra, Elizabet Amanda Lorena Baravalle, Celina Camussone, Cecilia Monecke, Stefan Calvinho, Luis Fernando Dallard, Bibiana Elisabet |
| author_browse | Baravalle, Celina Beccaria, Camila Calvinho, Luis Fernando Camussone, Cecilia Dallard, Bibiana Elisabet Engler, Carolina Monecke, Stefan Pereyra, Elizabet Amanda Lorena Pirola, Silvana I. Renna, María Sol Silvestrini, Paula |
| author_facet | Engler, Carolina Renna, María Sol Beccaria, Camila Silvestrini, Paula Pirola, Silvana I. Pereyra, Elizabet Amanda Lorena Baravalle, Celina Camussone, Cecilia Monecke, Stefan Calvinho, Luis Fernando Dallard, Bibiana Elisabet |
| author_sort | Engler, Carolina |
| collection | INTA Digital |
| description | The aim of this study was to evaluate and compare the ability of two S. aureus strains with different adaptation genotypes (low and high) to the bovine mammary gland (MG) to establish an intramammary infection (IMI) and induce an immune response after an experimental challenge in lactating cows. Two isolates (designated 806 and 5011) from bovine IMI with different genotypic profiles, harboring genes involved in adherence and biofilm production, belonging to different capsular polysaccharide (CP) type, accessory gene regulator (agr) group, pulsotype (PT) and sequence type/clonal complex (ST/CC) were selected. Strains 806 and 5011 were associated with low (nonpersistent-NP) and high (persistent-P) adaptation to the MG, respectively. Strain 806 (NP) was characterized as agr group II, cap5 positive and ST350; strain 5011 (P) agr group I, cap8 positive and CC188. Three groups of clinically healthy cows, 4 cows/treatment group, were inoculated by the intramammary route with strain 806 (NP), strain 5011 (P) and pyrogen-free saline solution. All mammary quarters challenged with strain 806 (NP) developed mild clinical mastitis between 1 and 7 d post inoculation (pi). Quarters challenged with strain 5011 (P) developed a persistent IMI; bacteria were recovered from milk from d 7 pi and up to d 56 pi. In quarters inoculated with strain 806 (NP) the inflammatory response induced was greater and earlier than the one induced by strain 5011 (P), since a somatic cell count (SCC) peak was observed at d 2 pi, while in quarters inoculated with strain 5011 (P) no variations in SCC were observed until d 4 pi reaching the maximum values at d 14 pi; indicating a lower and delayed initial inflammatory response. The highest levels of nitric oxide (NO) and lactoferrin (Lf) detected in milk from quarters inoculated with both S. aureus strains coincided with the highest SCC at the same time periods, indicating an association with the magnitude of inflammation. The high levels of IL-1β induced by strain 806 (NP) were associated with the highest SCC detected (d 2 pi); while quarters inoculated with strain 5011 (P) showed similar IL-1β levels to those found in control quarters. In quarters inoculated with strain 806 (NP) two peaks of IL-6 levels on d 2 and 14 pi were observed; while in quarters inoculated with strain 5011 (P) IL-6 levels were similar to those found in control quarters. The strain 806 (NP) induced a higher total IgG and IgG1 response; while strain 5011 (P) generated a higher IgG2 response (even against the heterologous strain). The present study demonstrated that S. aureus strains with different genotype and adaptability to bovine MG influence the local host immune response and the course and severity of the infectious process. |
| format | info:ar-repo/semantics/artículo |
| id | INTA13892 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA138922023-01-12T11:31:45Z Differential immune response to two Staphylococcus aureus strains with distinct adaptation genotypes after experimental intramammary infection of dairy cows Engler, Carolina Renna, María Sol Beccaria, Camila Silvestrini, Paula Pirola, Silvana I. Pereyra, Elizabet Amanda Lorena Baravalle, Celina Camussone, Cecilia Monecke, Stefan Calvinho, Luis Fernando Dallard, Bibiana Elisabet Bovine Mastitis Strains Dairy Cows Immune Response Genotypes Mastitis Bovina Staphylococcus aureus Cepas Vacas Lecheras Respuesta Inmunológica Genotipos Persistent and Nonpersistent Strains Cepas Persistentes y no Persistentes The aim of this study was to evaluate and compare the ability of two S. aureus strains with different adaptation genotypes (low and high) to the bovine mammary gland (MG) to establish an intramammary infection (IMI) and induce an immune response after an experimental challenge in lactating cows. Two isolates (designated 806 and 5011) from bovine IMI with different genotypic profiles, harboring genes involved in adherence and biofilm production, belonging to different capsular polysaccharide (CP) type, accessory gene regulator (agr) group, pulsotype (PT) and sequence type/clonal complex (ST/CC) were selected. Strains 806 and 5011 were associated with low (nonpersistent-NP) and high (persistent-P) adaptation to the MG, respectively. Strain 806 (NP) was characterized as agr group II, cap5 positive and ST350; strain 5011 (P) agr group I, cap8 positive and CC188. Three groups of clinically healthy cows, 4 cows/treatment group, were inoculated by the intramammary route with strain 806 (NP), strain 5011 (P) and pyrogen-free saline solution. All mammary quarters challenged with strain 806 (NP) developed mild clinical mastitis between 1 and 7 d post inoculation (pi). Quarters challenged with strain 5011 (P) developed a persistent IMI; bacteria were recovered from milk from d 7 pi and up to d 56 pi. In quarters inoculated with strain 806 (NP) the inflammatory response induced was greater and earlier than the one induced by strain 5011 (P), since a somatic cell count (SCC) peak was observed at d 2 pi, while in quarters inoculated with strain 5011 (P) no variations in SCC were observed until d 4 pi reaching the maximum values at d 14 pi; indicating a lower and delayed initial inflammatory response. The highest levels of nitric oxide (NO) and lactoferrin (Lf) detected in milk from quarters inoculated with both S. aureus strains coincided with the highest SCC at the same time periods, indicating an association with the magnitude of inflammation. The high levels of IL-1β induced by strain 806 (NP) were associated with the highest SCC detected (d 2 pi); while quarters inoculated with strain 5011 (P) showed similar IL-1β levels to those found in control quarters. In quarters inoculated with strain 806 (NP) two peaks of IL-6 levels on d 2 and 14 pi were observed; while in quarters inoculated with strain 5011 (P) IL-6 levels were similar to those found in control quarters. The strain 806 (NP) induced a higher total IgG and IgG1 response; while strain 5011 (P) generated a higher IgG2 response (even against the heterologous strain). The present study demonstrated that S. aureus strains with different genotype and adaptability to bovine MG influence the local host immune response and the course and severity of the infectious process. EEA Rafaela Fil: Engler, Carolina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Biología Celular y Molecular Aplicada; Argentina Fil: Engler, Carolina. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral (ICIVET-Litoral); Argentina Fil: Renna, Maria S. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Biología Celular y Molecular Aplicada; Argentina Fil: Renna, Maria S. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral (ICIVET-Litoral); Argentina Fil: Beccaria, Camila. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Biología Celular y Molecular Aplicada; Argentina Fil: Beccaria, Camila. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral (ICIVET-Litoral); Argentina Fil: Silvestrini, Paula. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Biología Celular y Molecular Aplicada; Argentina Fil: Silvestrini, Paula. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral (ICIVET-Litoral); Argentina Fil: Pirola, Silvana. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Biología Celular y Molecular Aplicada; Argentina Fil: Pirola, Silvana. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral (ICIVET-Litoral); Argentina Fil: Pereyra, Elizabeth A.L. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Biología Celular y Molecular Aplicada; Argentina Fil: Pereyra, Elizabeth A.L. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral (ICIVET-Litoral); Argentina Fil: Baravalle, Celina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Biología Celular y Molecular Aplicada; Argentina Fil: Baravalle, Celina. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral (ICIVET-Litoral); Argentina Fil: Camussone, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina. Fil: Camussone, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina. Fil: Monecke, Stefan. Technische Universität Dresden (TU Dresden). Institute for Medical Microbiology and Hygiene; Alemania Fil: Monecke, Stefan. Alere Technologies GmbH.; Alemania Fil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina. Fil: Calvinho, Luis Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina. Fil: Dallard, Bibiana Elisabet. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Biología Celular y Molecular Aplicada; Argentina Fil: Dallard, Bibiana Elisabet. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral (ICIVET-Litoral); Argentina 2023-01-12T11:08:48Z 2023-01-12T11:08:48Z 2022-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/13892 https://www.sciencedirect.com/science/article/pii/S0882401022004028 0882-4010 https://doi.org/10.1016/j.micpath.2022.105789 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Elsevier Microbial Pathogenesis 172 : 105789 (November 2022) |
| spellingShingle | Bovine Mastitis Strains Dairy Cows Immune Response Genotypes Mastitis Bovina Staphylococcus aureus Cepas Vacas Lecheras Respuesta Inmunológica Genotipos Persistent and Nonpersistent Strains Cepas Persistentes y no Persistentes Engler, Carolina Renna, María Sol Beccaria, Camila Silvestrini, Paula Pirola, Silvana I. Pereyra, Elizabet Amanda Lorena Baravalle, Celina Camussone, Cecilia Monecke, Stefan Calvinho, Luis Fernando Dallard, Bibiana Elisabet Differential immune response to two Staphylococcus aureus strains with distinct adaptation genotypes after experimental intramammary infection of dairy cows |
| title | Differential immune response to two Staphylococcus aureus strains with distinct adaptation genotypes after experimental intramammary infection of dairy cows |
| title_full | Differential immune response to two Staphylococcus aureus strains with distinct adaptation genotypes after experimental intramammary infection of dairy cows |
| title_fullStr | Differential immune response to two Staphylococcus aureus strains with distinct adaptation genotypes after experimental intramammary infection of dairy cows |
| title_full_unstemmed | Differential immune response to two Staphylococcus aureus strains with distinct adaptation genotypes after experimental intramammary infection of dairy cows |
| title_short | Differential immune response to two Staphylococcus aureus strains with distinct adaptation genotypes after experimental intramammary infection of dairy cows |
| title_sort | differential immune response to two staphylococcus aureus strains with distinct adaptation genotypes after experimental intramammary infection of dairy cows |
| topic | Bovine Mastitis Strains Dairy Cows Immune Response Genotypes Mastitis Bovina Staphylococcus aureus Cepas Vacas Lecheras Respuesta Inmunológica Genotipos Persistent and Nonpersistent Strains Cepas Persistentes y no Persistentes |
| url | http://hdl.handle.net/20.500.12123/13892 https://www.sciencedirect.com/science/article/pii/S0882401022004028 https://doi.org/10.1016/j.micpath.2022.105789 |
| work_keys_str_mv | AT englercarolina differentialimmuneresponsetotwostaphylococcusaureusstrainswithdistinctadaptationgenotypesafterexperimentalintramammaryinfectionofdairycows AT rennamariasol differentialimmuneresponsetotwostaphylococcusaureusstrainswithdistinctadaptationgenotypesafterexperimentalintramammaryinfectionofdairycows AT beccariacamila differentialimmuneresponsetotwostaphylococcusaureusstrainswithdistinctadaptationgenotypesafterexperimentalintramammaryinfectionofdairycows AT silvestrinipaula differentialimmuneresponsetotwostaphylococcusaureusstrainswithdistinctadaptationgenotypesafterexperimentalintramammaryinfectionofdairycows AT pirolasilvanai differentialimmuneresponsetotwostaphylococcusaureusstrainswithdistinctadaptationgenotypesafterexperimentalintramammaryinfectionofdairycows AT pereyraelizabetamandalorena differentialimmuneresponsetotwostaphylococcusaureusstrainswithdistinctadaptationgenotypesafterexperimentalintramammaryinfectionofdairycows AT baravallecelina differentialimmuneresponsetotwostaphylococcusaureusstrainswithdistinctadaptationgenotypesafterexperimentalintramammaryinfectionofdairycows AT camussonececilia differentialimmuneresponsetotwostaphylococcusaureusstrainswithdistinctadaptationgenotypesafterexperimentalintramammaryinfectionofdairycows AT moneckestefan differentialimmuneresponsetotwostaphylococcusaureusstrainswithdistinctadaptationgenotypesafterexperimentalintramammaryinfectionofdairycows AT calvinholuisfernando differentialimmuneresponsetotwostaphylococcusaureusstrainswithdistinctadaptationgenotypesafterexperimentalintramammaryinfectionofdairycows AT dallardbibianaelisabet differentialimmuneresponsetotwostaphylococcusaureusstrainswithdistinctadaptationgenotypesafterexperimentalintramammaryinfectionofdairycows |