A successful vitrification technique for goat morulae conservation
The aim of this study was to evaluate the effect of different vitrification and warming processes on the in vitro embryo survival of caprine morulae, considering the day of recovery. A total of 136 morulae of Criolla-Neuquina goats recovered on Days 7 or 8 after sponge removal, were exposed to three...
| Autores principales: | , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Elsevier
2023
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/13847 https://www.sciencedirect.com/science/article/abs/pii/S0093691X22000437 https://doi.org/10.1016/j.theriogenology.2022.02.002 |
| _version_ | 1855037012882489344 |
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| author | Fernandez, Jimena Bruno Galarraga, María Macarena Lacau Megido, Isabel María Cueto, Marcela Isabel Gibbons, Alejandro Eduardo |
| author_browse | Bruno Galarraga, María Macarena Cueto, Marcela Isabel Fernandez, Jimena Gibbons, Alejandro Eduardo Lacau Megido, Isabel María |
| author_facet | Fernandez, Jimena Bruno Galarraga, María Macarena Lacau Megido, Isabel María Cueto, Marcela Isabel Gibbons, Alejandro Eduardo |
| author_sort | Fernandez, Jimena |
| collection | INTA Digital |
| description | The aim of this study was to evaluate the effect of different vitrification and warming processes on the in vitro embryo survival of caprine morulae, considering the day of recovery. A total of 136 morulae of Criolla-Neuquina goats recovered on Days 7 or 8 after sponge removal, were exposed to three different vitrification processes; V1 (n ¼ 48): glycerol (G) þ ethylene glycol (EG); V2 (n ¼ 44): EG þ 0.5 M sucrose and V3 (n ¼ 44): G þ EG þ 0.5 M sucrose. The morulae of each vitrification process were randomly assigned to three warming processes; W1 (n ¼ 45): 0.5 M sucrose at 25°C; W2 (n ¼ 44): 0.5 M sucrose at 39°C; and W3 (n ¼ 47): solution containing half the concentration of the cryoprotectants þ 0.5 M sucrose at 25°C. After, embryos were cultured in 100 mL TCM 199 drops under mineral oil, at 39°C and a 6.5% CO2 atmosphere for 72 h according to the different treatments. There were no viable embryos in V1
and V2 in none of their three respective warmings. Only V3 showed an embryo survival rate to hatched blastocyst stage of 59.1%. When considering embryo survival according to the warming processes, the survival rate was higher in V3W2 (76.9%) and V3W3 (66.7%) groups compared to the V3W1 group (37.5%; P < 0.05). The embryo survival of V3 for Day 8 after sponge removal (81.3%) was higher compared to Day 7 (46.4%; P < 0.05). In conclusion, a successful embryo survival is obtained by using a combination of
cryoprotectants (G þ EG) with addition of sucrose in the vitrification process for conservation of caprine morulae in embryo transfer programs. The survival rates in vitro of vitrified-warmed morulae in goats were influenced by their recovery day. Further studies should be conducted to determine if these results are reproducible in vivo embryo transfer on field situations. |
| format | info:ar-repo/semantics/artículo |
| id | INTA13847 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA138472023-01-09T10:20:53Z A successful vitrification technique for goat morulae conservation Fernandez, Jimena Bruno Galarraga, María Macarena Lacau Megido, Isabel María Cueto, Marcela Isabel Gibbons, Alejandro Eduardo Criopreservación Reproducción Animal Inseminación Artificial Caprinos Cryopreservation Animal Reproduction Artificial Insemination Goats The aim of this study was to evaluate the effect of different vitrification and warming processes on the in vitro embryo survival of caprine morulae, considering the day of recovery. A total of 136 morulae of Criolla-Neuquina goats recovered on Days 7 or 8 after sponge removal, were exposed to three different vitrification processes; V1 (n ¼ 48): glycerol (G) þ ethylene glycol (EG); V2 (n ¼ 44): EG þ 0.5 M sucrose and V3 (n ¼ 44): G þ EG þ 0.5 M sucrose. The morulae of each vitrification process were randomly assigned to three warming processes; W1 (n ¼ 45): 0.5 M sucrose at 25°C; W2 (n ¼ 44): 0.5 M sucrose at 39°C; and W3 (n ¼ 47): solution containing half the concentration of the cryoprotectants þ 0.5 M sucrose at 25°C. After, embryos were cultured in 100 mL TCM 199 drops under mineral oil, at 39°C and a 6.5% CO2 atmosphere for 72 h according to the different treatments. There were no viable embryos in V1 and V2 in none of their three respective warmings. Only V3 showed an embryo survival rate to hatched blastocyst stage of 59.1%. When considering embryo survival according to the warming processes, the survival rate was higher in V3W2 (76.9%) and V3W3 (66.7%) groups compared to the V3W1 group (37.5%; P < 0.05). The embryo survival of V3 for Day 8 after sponge removal (81.3%) was higher compared to Day 7 (46.4%; P < 0.05). In conclusion, a successful embryo survival is obtained by using a combination of cryoprotectants (G þ EG) with addition of sucrose in the vitrification process for conservation of caprine morulae in embryo transfer programs. The survival rates in vitro of vitrified-warmed morulae in goats were influenced by their recovery day. Further studies should be conducted to determine if these results are reproducible in vivo embryo transfer on field situations. Estación Experimental Agropecuaria Bariloche Fil: Fernandez, Jimena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Area Produccion Animal; Argentina Fil: Fernandez, Jimena. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina Fil: Bruno Galarraga, María Macarena. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estacion Experimental Agropecuaria Bariloche. Area Produccion Animal; Argentina Fil: Lacau Megido, Isabel María. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Biología y Medicina Experimental. Laboratorio de Regulación Hipofisaria; Argentina Fil: Cueto, Marcela Isabel. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estacion Experimental Agropecuaria Bariloche. Area Produccion Animal; Argentina Fil: Gibbons, Alejandro Eduardo. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estacion Experimental Agropecuaria Bariloche. Area Produccion Animal. Grupo de Reproducción y Genética; Argentina 2023-01-09T10:16:05Z 2023-01-09T10:16:05Z 2022-04-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/13847 https://www.sciencedirect.com/science/article/abs/pii/S0093691X22000437 0093-691X 1879-3231 https://doi.org/10.1016/j.theriogenology.2022.02.002 eng info:eu-repograntAgreement/INTA/2019-PD-E5-I107-001/2019-PD-E5-I107-001/AR./Biotecnologías de la reproducción: mejora de la eficiencia reproductiva en especies de interés zootécnico info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Theriogenology 182 : 103-109 (April 2022) |
| spellingShingle | Criopreservación Reproducción Animal Inseminación Artificial Caprinos Cryopreservation Animal Reproduction Artificial Insemination Goats Fernandez, Jimena Bruno Galarraga, María Macarena Lacau Megido, Isabel María Cueto, Marcela Isabel Gibbons, Alejandro Eduardo A successful vitrification technique for goat morulae conservation |
| title | A successful vitrification technique for goat morulae conservation |
| title_full | A successful vitrification technique for goat morulae conservation |
| title_fullStr | A successful vitrification technique for goat morulae conservation |
| title_full_unstemmed | A successful vitrification technique for goat morulae conservation |
| title_short | A successful vitrification technique for goat morulae conservation |
| title_sort | successful vitrification technique for goat morulae conservation |
| topic | Criopreservación Reproducción Animal Inseminación Artificial Caprinos Cryopreservation Animal Reproduction Artificial Insemination Goats |
| url | http://hdl.handle.net/20.500.12123/13847 https://www.sciencedirect.com/science/article/abs/pii/S0093691X22000437 https://doi.org/10.1016/j.theriogenology.2022.02.002 |
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